石质文物加固中细菌诱导碳酸钙生成的研究

为探讨细菌生物矿化法加固石质文物,利用枯草杆菌-Ⅱ在特殊含钙培养基上进行诱导矿化研究.结果表明,筛选出的菌株枯草杆菌-Ⅱ在M-3+ASP固体和液体培养基上能诱导生成白色物;白色诱导物经X-衍射分析为碳酸钙;电子显微镜下观察发现碳酸钙在细菌周围生成,并包裹细菌菌体,使细菌菌体钙化.

Study of stimulated calcite precipitation by bacteria in historic stone consolidation

With the aim of using bacterial biomineralization to reinforce stone relics,Bacillus subtilis was selected to study stimulated biomineralization with calcite(calcium carbonate)in special cultures containing calcium.The results showed that Bacillus subtilis could induce calcite precipition in solid and liquid M-3+ASP cultures.The white precipitation was shown to be calcite crystals by XRD analysis.Under optical and scanning electron microcopy(SEM),calcite was observed around the bacterial cells,enwrapping and calcified them.