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Ancient DNA in archaeological wheat grains: preservation conditions and the study of pre-Hispanic agriculture on the island of Gran Canaria (Spain) |
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| Authors: | Hugo R Oliveira Peter Civáň Jacob MoralesAmelia Rodríguez-Rodríguez Diane L ListerMartin K Jones |
| Institution: | a Department of Archaeology, University of Cambridge, Downing Street, Cambridge CB2 3DZ, UK b Manchester Interdisciplinary Biocentre, Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK c Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská Dolina, 842 15 Bratislava, Slovakia d Departamento de Ciencias Históricas, University of Las Palmas de Gran Canaria, c/Pérez del Toro, CP 35003 Las Palmas de Gran Canaria, Spain e McDonald Institute for Archaeological Research, Downing Street, Cambridge CB2 3ER, UK |
| Abstract: | The recovery of ancient DNA from archaeological wheat samples under different preservation conditions was assessed using a number of genetic markers. It was possible to amplify nuclear DNA from desiccated grains but not from charred. The desiccated grain was from a pre-Hispanic grain silo in Gran Canaria and showed excellent DNA preservation, enabling the amplification of the ribosomal DNA markers IGS and ITS, the upstream region of the HMW-glutenin locus and single-locus nuclear microsatellites. Our results demonstrated the presence of both durum and bread wheat in an assemblage of naked grain. We were also able to identify different genotypes in durum wheat and compare these with extant landraces, providing insights into the agrarian practices of the ancient Canarians and the origin of their crops. |
| Keywords: | Wheat Ancient DNA Preservation Microsatellites Canary Islands Agriculture |
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