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馆藏纸质书画文物上霉菌的分离与鉴定
引用本文:唐 欢,王 春,范文奇,周理坤,马冠华.馆藏纸质书画文物上霉菌的分离与鉴定[J].文物保护与考古科学,2015,27(2):40-46.
作者姓名:唐 欢  王 春  范文奇  周理坤  马冠华
作者单位:重庆中国三峡博物馆,重庆400015,重庆中国三峡博物馆,重庆400015,重庆中国三峡博物馆,重庆400015,重庆中国三峡博物馆,重庆400015,西南大学植物保护学院,重庆400715
基金项目:重庆中国三峡博物馆项目资助(2013KTZ01)
摘    要:霉菌污染会影响馆藏纸质书画的保存和展示。对书画污染菌进行分离及鉴定,有助于提高文物霉变防治工作的针对性,进而提高防治效果。本研究利用传统的纯培养法从遭受霉菌污染的馆藏纸质书画表面分离得到8株霉菌,通过形态特征观察和r DNA转录间隔区序列ITS(Internal Transcribed Spacer)测序分析。结果表明:8株霉菌分属于曲霉属(Aspergillus)、根霉属(Rhizopu)和木霉属(Trichoderma)。研究结果可为馆藏纸质书画文物防霉剂的研发和文物霉菌的综合防治提供靶标和依据。

关 键 词:霉菌  书画  文物  分离与鉴定  rDNAITS

Isolation and identification of molds from ancient Chinese calligraphy and paintings in museums
TANG Huan,WANG Chun,FAN Wenqi,ZHOU Likun and MA Guanhua.Isolation and identification of molds from ancient Chinese calligraphy and paintings in museums[J].Sciences of Conservation and Archaeology,2015,27(2):40-46.
Authors:TANG Huan  WANG Chun  FAN Wenqi  ZHOU Likun and MA Guanhua
Institution:China Three Gorges Museum, Chongqing,400015,China Three Gorges Museum, Chongqing,400015,China Three Gorges Museum, Chongqing,400015,China Three Gorges Museum, Chongqing,400015 and College of Plant Protection, Southwest University, Chongqing 400716
Abstract:Contamination by mold is a major potential risk for ancient Chinese calligraphy and paintings on display in museums. The isolation and identification of these microorganisms is helpful for developing a useful approach to control deterioration. In this study, the traditional pure culturedependent method was performed, and 8 strains of mold were isolated from the paper paintings. In addition to typical mold characteristics, they were identified by ITS sequencing. The results of the morphological and molecular biological investigations show that Aspergillus spp.,Rhizopus spp. and Trichoderma spp. are present in these in these specimens.
Keywords:Mold  Calligraphy and painting heritage  Cultural relics  Isolation and identification  rDNAITS
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