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Amplification and sequencing of Trichuris trichiura ancient DNA extracted from archaeological sediments |
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| Authors: | Chang Seok Oh Min Seo Jong Yil Chai Sang Jun Lee Myeung Ju Kim Jun Bum Park Dong Hoon Shin |
| Affiliation: | 1. Institute of Forensic Medicine, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, Republic of Korea;2. Department of Parasitology, Dankook University College of Medicine, San 29, Anseo-Dong, Chonan 330-714, Republic of Korea;3. Department of Parasitology, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, Republic of Korea;4. Department of Anatomy, Dankook University College of Medicine, San 29, Anseo-Dong, Chonan 330-714, Republic of Korea;5. Hangang Institute of Cultural Heritage, 294-6, Jungwha 1 Dong, Seoul 110-799, Republic of Korea |
| Abstract: | Despite high prevalence of Trichuris trichiura infection, PCR-based analysis on T. trichiura from archaeological samples has not been established so far. In the present study, we sought to perform PCR-based amplification of T. trichiura aDNA using the sediments from medieval tomb of Korea. The presence of Trichuris eggs were first detected by microscopic observation; then confirmed by PCR-based aDNA analysis. Obtained sequence showed 100% homology to that of the small subunit ribosomal RNA (SSUrRNA) gene of T. trichiura but distinct from that of other Trichuris species. PCR-based aDNA analysis in this study can serve as effective method to confirm the presence of T. trichiura eggs in the soils or coprolites collected from archaeological sites. |
| Keywords: | Trichuris trichiura Ancient DNA Korea PCR Cloning and sequencing |
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