High-throughput mass spectrometric analysis of 1400-year-old mycolic acids as biomarkers for ancient tuberculosis infection |
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| Authors: | Laszlo Mark Zoltan Patonai Alexandra Vaczy Tamas Lorand Antonia Marcsik |
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| Institution: | 1. Institute of Biochemistry and Medical Chemistry, Faculty of Medicine, University of Pecs, Szigeti str. 12, H-7624 Pecs, Hungary;2. Department of Anthropology, University of Szeged, Egyetem str. 2, H-6722 Szeged, Hungary |
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| Abstract: | The cell wall of mycobacteria includes an unusual outer membrane of extremely low permeability. This cell envelope consists of a characteristic cell wall skeleton, a mycoloyl arabinogalactan peptidoglycan complex, and related hydrophobic components that contribute to the cell surface properties. In this study 1400-year-old mycolic acids as unique tuberculosis biomarkers have been extracted and identified for the first time by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) and Fourier transform infrared spectroscopy (FT-IR). The data suggest that the MALDI TOF MS has potential as a rapid and reproducible technique for the detection and identification of ancient mycobacterial infections. |
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| Keywords: | Bone MALDI TOF MS MTC Mycobacterium tubeculosis Mycolic acid Paleopathology |
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