Contamination controls when preparing archaeological remains for ancient DNA analysis |
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| Affiliation: | 1. Department of Anthropology, University of Oregon, USA;2. Kemp Laboratory of Molecular Anthropology and Ancient DNA, Washington State University, USA;3. Department of Anthropology, Washington State University, USA;4. School of Biological Sciences, Washington State University, USA;1. Department of Anthropology, Washington State University, Pullman, WA 99164-4236, USA;2. School of Biological Sciences, Washington State University, Pullman, WA 99164-4910, USA;3. Department of Anthropology, University of California-Santa Barbara, Santa Barbara, CA 93106-3210, USA;4. Kemp Lab of Molecular Anthropology and Ancient DNA, Washington State University, Pullman, WA 99164-4236, USA;1. BioArCh, Department of Archaeology, Biology S Block, University of York, York, YO10 5DD, UK;2. Bioscience Technology Facility, Department of Biology, University of York, Heslington, York, YO10 5YW, UK;3. Centre for Clinical Microbiology, University College London, Royal Free Campus, London, NW3 2PF, UK;4. Hungarian Natural History Museum, Department of Anthropology, H–1431, Budapest, Pf. 137, Hungary;5. Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK;6. Kuvin Center for the Study of Infectious and Tropical Diseases and Ancient DNA, Hadassah Medical School, Hebrew University, Jerusalem, Israel;7. Faculty of Life Sciences, Manchester Institute of Biotechnology, University of Manchester, John Garside Building, 131 Princess Street, Manchester, M1 7DN, UK;8. Department of Chemistry, University of York, York, YO10 5DD, UK;9. Centre of Excellence in Mass Spectrometry, University of York, York, YO10 5DD, UK;1. School of Human Evolution and Social Change, Tempe, AZ, United States;2. Center for Evolution and Medicine, Tempe, AZ, United States;3. Institute for Human Origins, Tempe, AZ, United States;1. Ancient DNA Laboratory, Department of Archaeology, Simon Fraser University, 8888 University Drive, Burnaby, BC V5A 1S6, Canada;2. Department of Anthropology, Trent University, 1600 West Bank Drive, Peterborough, ON K9L 0G2, Canada;3. Department of Anthropology, University of British Columbia, 6306 NW Marine Drive, Vancouver, BC V6T 1Z1, Canada;4. Department of Anthropology, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, ON L5L 1C6, Canada;5. Perca Zooarchaeological Research, Toronto, ON, Canada;6. The Archaeology Centre, University of Toronto, 19 Russell Street, Toronto, ON M5S 2S2, Canada;7. Trent University Archaeological Research Centre, Trent University, 1600 West Bank Drive, Peterborough, ON K9L 0G2, Canada |
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| Abstract: | Contamination is of utmost concern when working with ancient DNA as it easily leads to false positive results. The best way to prevent or minimize contamination is to start precautionary measures as early as possible, ideally commencing with sample collection and preparation by field archaeologists. This paper discusses the nature of contamination in ancient DNA studies and offers some practical guidelines as to how archaeologists in the field can “clean-collect” samples for ancient DNA analysis. Methods for preparing contaminated samples from museum collections for ancient DNA analysis are also discussed. |
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