Independent confirmation of a diagnostic sheep/goat peptide sequence through DNA analysis and further exploration of its taxonomic utility within the Bovidae |
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| Authors: | Michael G Campana Terence Robinson Paula F Campos Noreen Tuross |
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| Institution: | 1. Department of Human Evolutionary Biology, Harvard University, Peabody Museum, 11 Divinity Ave., Cambridge, MA 02138, USA;2. Department of Botany and Zoology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa;3. Centre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 1350 Copenhagen, Denmark;4. Museu da Ciência, Universidade de Coimbra, Laboratorio Chimico, Largo Marquês de Pombal, 3000-272 Coimbra, Portugal |
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| Abstract: | Buckley et al. (2010) recently identified a Type I collagen (COL1A2) peptide sequence that discriminates between sheep and goat species. The exact location of this peptide sequence on the genome was not reported. We identified the sequence's location and developed a PCR based approach that amplifies the diagnostic sequence from exon 41 of the COL1A2 gene. Using DNA analysis, we confirmed that this COL1A2 peptide discriminates between goat and sheep species reliably, but is limited as a more general phylogenetic marker. |
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