壁画、建筑彩绘文物中几种测定蛋白质方法的比较和评价

蛋白质常作为胶结材料存在于壁画、建筑彩绘文物中。它的老化变质及其流失会使彩绘颜料产生掉色、脱落,胶结材料的含量与该类文物的病害密切相关。因此,定量测定蛋白质含量对彩绘文物的病害机理和修复保护研究具有重要意义。本研究在总结凯氏定氮法、分光光度法等蛋白质含量测定的一般方法的基础上,重点阐述了彩绘文物颜料层和古建油饰彩画地仗层中蛋白质含量测定的常用方法,如气-质联用法、氨基酸分析法、元素分析法。介绍了这些方法的原理、特点以及应用实例。从分析结果来看,以上方法在彩绘文物颜料层和地仗材料中蛋白质含量测定方面发挥着重要作用。

Comparison and evaluation of methods for quantitative analysis of proteins in wall paintings and colored relics of ancient architecture

Protein materials were usually used as binding media in wall paintings and colored relics of ancient architecture, and therefore their aging and deterioration may cause fading and peeling-off of pigments.The relative content of binding media is closely related to the decay of this type of cultural heritage.Therefore,quantitative analysis of proteins is very important for the study of decay mechanisms,restoration and conservation of painted cultural heritage objects.This article summarizes the methods used f...