The use of the polymerase chain reaction (PCR) to detect Mycobacterium tuberculosis in ancient skeletons |
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Authors: | Mark Spigelman Eshetu Lemma |
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Abstract: | The polymerase chain reaction has been used to extract ancient DNA from a wide range of different types of material. We have considered the possibility of finding residual bacterial DNA in bone that may have been infected with Mycobacterium tuberculosis using this technique. We propose a method of collecting samples and testing for the presence of degraded genetic material in ancient bone. The steps of the polymerase chain reaction are detailed and discussed, as are those for the preparation of the bone sample. Results obtained would suggest that this technique could have wide application in osteoarchaeological research by giving us new information on diseases of antiquity. Future avenues for the investigation of bacterial DNA in ancient bone are suggested. |
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Keywords: | Polymerase chain reaction (PCR) DNA Mycobacterium tuberculosis ancient bones |
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