ATP生物发光法在文物抑菌剂效力检测中的应用

抑菌剂的筛选是文物微生物病害治理的一项重要内容。传统的效力检测方法依赖培养,因而不能用于人工培养条件下生长状态不佳的微生物,更无法用于文物现场的快速检测。近年兴起的一些活菌快速检测技术,由于不依赖培养,为筛选文物保护抑菌剂提供了新的思路。本工作总结了传统方法以及当前常用的活菌快速检测技术,通过实验室及现场实验,实现了用ATP生物发光检测文物抑菌剂效力,弥补了传统技术的不足,为筛选文物保护抑菌剂提供了新的方法。

Application of ATPbioluminescence method for the evaluation of biocides used for cultural heritage conservation

Selection of an effective biocide to control microbial damage to relics is important for cultural heritage conservation. Traditionally, selection methods depend on growing bacteria under controlled conditions in lab. However, some microorganisms cannot be studied well because they do not grow well under these conditions. Furthermore, the methods cannot be applied for rapid onsite detection and identification of certain microorganisms. Recently, some rapid detection methods for viable microbes have been developed that do not require cultivation and can solve these problems. This study reviews traditional and current rapid detection methods. It is found that an adenosine triphosphate (ATP) bioluminescence technique, works very well, both in the lab and onsite for the evaluation of the effects of multiple biocides on relics. The method provides a new way to select biocides for cultural heritage conservation.