Hemp in ancient rope and fabric from the Christmas Cave in Israel: talmudic background and DNA sequence identification |
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| Authors: | Terence M. Murphy Nahum Ben-Yehuda R.E. Taylor John R. Southon |
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| Affiliation: | 1. Department of Plant Biology, University of California, One Shields Avenue, Davis, CA 95616, USA;2. Department of Jewish History, CText ATI, Bar Ilan University, Ramat Gan 52900, Israel;3. Department of Anthropology, University of California, Riverside, CA 92521, USA;4. Cotsen Institute of Archaeology, University of California, Los Angeles, CA 90095, USA;5. Keck Accelerator Mass Spectrometry Laboratory, University of California, Irvine, CA 92697, USA;6. Department of Earth System Science, University of California, Keck Accelerator Mass Spectrometry Laboratory, Irvine, CA 92697, USA |
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| Abstract: | The “Christmas Cave”, a cave in the Qidron Valley near the Dead Sea and Qumran, has yielded a complex collection of plant-derived rope and fabric artifacts. Using polymerase chain reaction (PCR) to amplify DNA of the samples, we estimated the sizes and determined restriction patterns and base sequences of chloroplast genes, primarily rbcL (gene for the large subunit of ribulose bisphosphate carboxylase). DNA was successfully extracted from all samples, but was limited to sizes of approximately 200–300 base pairs. As expected, the DNA extracted from the samples was identified as coming primarily from flax (Linum usitatissamum L.), but two samples had a significant fraction, and all samples had at least a trace, of hemp (Cannabis sativa L.) DNA. Artifacts from the Christmas Cave were thought to date from Roman times, but it was thought possible that some could be much older. Accelerator mass spectrometry (AMS)-based 14C dating confirmed that the samples contained representatives from both the Roman and Chalcolithic periods. This paper provides a synthesis of DNA, isotope, and literary analysis to illuminate textile history at the Christmas Cave site. |
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| Keywords: | Flax Linen Hemp Rope Fabric Israel Judean desert DNA rbcL gene PCR Polymerase chain reaction AMS-based 14C dating |
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