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DNA AND PROTEIN RECOVERY FROM WASHED EXPERIMENTAL STONE TOOLS*
Authors:O C SHANKS  M KORNFELD  W REAM
Abstract:Traces of protein and DNA are preserved on stone tools used to process animals. Previous research documents the identification of protein residues from tools sonicated in 5% ammonium hydroxide, but it remains untested whether the same treatment yields useable DNA. In this study we report both DNA and protein recovery using 5% ammonium hydroxide from residues on stone tools. We extracted 13‐year‐old residues from experimentally manufactured stone tools used to butcher a single animal. We also show that surface washing procedures typically used to curate stone tools remove only a small fraction of the DNA and protein deposited during animal butchery.
Keywords:POLYMERASE CHAIN REACTION  ANCIENT DNA  BLOOD RESIDUES  PROTEIN ANALYSIS  LITHICS  STONE TOOL FUNCTION
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