Are Teeth Better? Histological Characterization of Diagenesis in Archaeological Bone–Tooth Pairs and a Discussion of the Consequences for Archaeometric Sample Selection and Analyses

Teeth are often the preferred source material for isotopic and genetic assay involving ancient biomolecules. The assumption is that dental tissue preserves better due to its anatomically protected location, the enamel cap, and lower porosity compared to bone. However, this assumption has not been widely tested. Some similarities in diagenetic processes are to be expected due to similarities in structure and chemical composition of dentine and bone. This has led to the suggestion that bone can be used as an indicator of dental preservation, as a pre‐screening technique in the selection of suitable samples for biomolecular studies. Thus, direct testing of the correlation between bone and tooth preservation and diagenesis is needed. This paper reports the results of the histological characterization of diagenetic alterations within 25 human femur–tooth pairs, from a Medieval to modern (AD 1850) cemetery in Eindhoven, the Netherlands. The results showed that teeth do indeed preserve better overall, but not always, and that this was dependent on the main diagenetic factor(s) at the burial location. Furthermore, good correlations are found between the microstructural preservation of bone and teeth; similar processes of decay were observed within bone and teeth of the same individual. Overall, the study demonstrated that histological analysis of bone is useful for the identification of degradation processes that affect biomolecular preservation in skeletal material. In this way, sample selection and analytical strategies can be optimized. Copyright © 2013 John Wiley & Sons, Ltd.     

Archaeozoological research on vertebrates in Central Europe with special reference to the medieval period

This paper is the outcome of a lecture held at the Institute of Archaeology, University College London. It provides a summary in English of the archaeozoological research work which has been carried out in Central Europe during the last 30 years, with special reference to material from medieval times. It is shown that a thorough zoological analysis of bones from excavations provides much information for historical interpretation. Not only was the ratio of wild and domestic animals or of the different species of interest, but also the age and sex structure of the population by taking the function of the site into consideration. Some observations on animal teeth gave indications of distinctive handicrafts. Cut marks on cervical vertebrae of horse skeletons found as grave goods from early medieval times were considered in connection with beliefs and juridical conceptions of that time.     

8500-year-old Late Mesolithic garment embroidery from Vlasac (Serbia): Technological,use-wear and residue analyses

This paper presents results of contextual, technological, use-wear and residue analyses of body ornaments from two Late Mesolithic burials recently excavated at the site of Vlasac in the Danube Gorges of the central Balkans. Common to both burials are ornaments made from modified and unmodified carp (Cyprinidae sp.) pharyngeal ‘teeth’ along with Cyclope neritea marine gastropods. Experimental and low and high magnification use-wear approaches have been employed in reconstructing the way these ornaments were made and used. The precise contextual distribution of these ornaments has been recorded for the first time. The two examined burials exhibit a number of similarities, particularly in the way ornaments were placed in relation to the body. Both burials are also contemporaneous, dated to the mid-7th millennium BC. Implications of these findings for Mesolithic foragers' corporeal symbolism, group identity and regional and long-distance acquisition networks are briefly examined.     

Late late Albian (Early Cretaceous) shark teeth from Annopol,Poland

Siversson, M. & Machalski, M., February 2017. Late late Albian (Early Cretaceous) shark teeth from Annopol, Poland. Alcheringa 41, 433–463.

Screen washing of the condensed phosporite-bearing sands at the top of the Albian succession at Annopol, Poland, produced 789 selachian teeth of which 264 are determinable to at least genus level. The sediment type and methods of processing prevented recovery of small-toothed taxa, resulting in an assemblage comprising 13, mostly large-toothed taxa. Lamniformes dominates with Dwardius sp. being, by far, the most common taxon. Observations on vertical distribution and preservation of the teeth (with focus on the adhered phosphatic matrix), coupled with biostratigraphic ranges of co-occurring ammonites, indicate that the majority of the shark material is attributable to the Mortoniceras rostratum or, more probably, M. perinflatum Zone (late late Albian; mid-‘Vraconnian’). This is compatible with the composition of the shark assemblage, characterized by the co-occurrence of Paraisurus sp. aff. P. compressus, Cretoxyrhina vraconensis and Squalicorax teeth with strong serrations on the cutting edges. The tightly curved basal edge of the root in lateral teeth of C. vraconensis conforms to that of teeth from the Pawpaw Formation of Texas (M. rostratum Zone) and differs from the more divergent root lobes in younger specimens from the uppermost Albian and/or lowermost Cenomanian of Kolbay, Mangyshlak. Some specimens in the studied assemblage are probably older, within the range from the middle to earliest late late Albian. The strong numerical dominance of either Cretoxyrhina or Dwardius in late late Albian to early Cenomanian selachian faunas indicates competitive exclusion in these similar-sized, apex predatory sharks.

Mikael Siversson* [mikael.siversson@museum.wa.gov.au], Department of Earth and Planetary Sciences, Western Australian Museum, 49 Kew Street, Welshpool, Western Australia 6106, Australia; Marcin Machalski [mach@twarda.pan.pl], Institute of Paleobiology, Polish Academy of Sciences, ul. Twarda 51/55, 00-818 Warszawa, Poland. *Also affiliated with: Department of Environment & Agriculture, Curtin University, Kent Street, Bentley, WA 6102, Australia.     

Canary islands aborigin sex determination based on mandible parameters contrasted by amelogenin analysis

Sex determination using mandible parameters is population dependent. In order to assess which measurements better characterize sex in prehispanic individuals from the Canary Islands, we blindly contrasted the results obtained by visual inspection and osteometric measurements with those obtained by molecular sexing using amelogenin ancient DNA analysis on teeth from the same material. Unambiguous sex classification was achieved by amplification of sex specific amelogenin alleles in 56 out of 76 mandibles (73.78% of the cases). Visual inspection led to a correct diagnosis in 66.04% of cases, with a greater proportion of errors for female (54.17%) than male (17.24%) mandibles. Osteometric measurements were able to assign sex correctly in 72.2% in the best of cases (mandibular height), a proportion similar to that obtained using a discriminant function (71.2%). By logistic regression analysis, ramus breadth, index ramus breadth/ramus height and mandibular length were the parameters independently related with a mistaken diagnosis of female sex, whereas bigonial width, ramus height and mandibular length were the parameters more closely and independently related to a mistaken diagnosis of male sex. In conclusion, diagnosis based on visual examination of the mandible or on its metric measurement only serves to roughly estimate sex with an accuracy of around 70% or less, at least among the prehispanic population from Gran Canaria. Amplification of amelogenin alleles leads to unambiguous identification of male and female alleles in 73.68% of cases, at least among the prehispanic population from Gran Canaria.     

Developmental Stress and Survival among the Mid Upper Paleolithic Sunghir Children: Dental Enamel Hypoplasias of Sunghir 2 and 3

The Sunghir 2 and 3 late juvenile to early adolescent immature skeletons, from the Mid Upper Paleolithic (Eastern Gravettian) of northern Russia, exhibit several episodes of dental enamel hypoplasias. Those of the older male Sunghir 2 relate principally to a stress episode in the third year post‐natal, although subsequent minor stress episodes may be indicated. They are not accompanied by other marked developmental abnormalities. The younger female Sunghir 3 exhibits at least three episodes of pronounced post‐natal stress through the third to fifth years post‐natal, followed by femoral and tibial growth arrest lines that formed shortly before her death at 9–11 years. These first decade post‐natal stress episodes of Sunghir 3 may be related to the same developmental condition that produced her abnormally bowed femora pre‐natally and/or to continued frailty related to that condition. Surviving these stress episodes for different lengths of time, both Sunghir 2 and 3 were awarded the most elaborate Paleolithic burial known. Copyright © 2011 John Wiley & Sons, Ltd.     

First report of theropods from the Tacuarembó Formation (Late Jurassic-Early Cretaceous), Uruguay

Dinosaur remains from the lower part of the Tacuarembó Formation, Uruguay, consist of the isolated theropod teeth described here. This is the first record of this group in the Tacuarembó Formation and represents the oldest dinosaur remains from Uruguay.     

Skeletal age at death: an evaluation of the miles method of ageing

The accuracy of Miles' method of ageing individuals within a population, based upon an analysis of the rate of dental wear, was evaluated in a sample of 202 living Lengua Indians from the Chaco area of Paraguay. The preliterate and culturally isolated status of the Lengua provided a useful base for comparison with populations that are likely to be encountered by archaeologists. The independent age estimates from maxillae and mandibles of the same individuals were found to be highly correlated with each other and to the actual ages of those individuals (Spearman rank-correlation test maxillae r_s = 0·58, mandibles r_s = 0·95, medians of populations rank-sum R = 99·5 > 95). No significant differences (P > 0·05) were found between the mean ages of any of the Miles subgroups, as indicated by the t-test for paired comparisons. The present study provides evidence of the reliability of the Miles method of ageing archaeological populations on the basis of occlusal wear. These findings are evaluated in the light of the sources of error inherent in the Miles system of analysis.     

Stress near the start of life? Localised enamel hypoplasia of the primary canine in late prehistoric mainland Southeast Asia

Localised hypoplasia of the primary canine (LHPC) is characterised by roughly circular defective areas of thinned or missing enamel on the labial surface. This defect is rarely reported in bioarchaeological research. Using samples from late prehistoric mainland Southeast Asia, this paper documents the prevalence of LHPC to produce baseline data for this defect. The samples are from seven archaeological sites in Thailand and collectively span from ca. 4000 to 1500 BP. In the combined samples, 32/79 (40.5%) of individuals and 47/199 (23.6%) of teeth had LHPC. The high occurrence of LHPC may suggest there was poor maternal and infant health. There is also a high occurrence of caries associated with LHPC, which has implications for the assessment of deciduous dental health. This paper stresses the importance of the collection of dental enamel defect data from deciduous teeth including LHPC in bioarchaeological research.     

Micro‐CT Imaging and Analysis of Enamel Defects on the Early Late Pleistocene Xujiayao Juvenile

Dental pathologies and enamel defects in East Asian hominins have rarely been reported. Here, we systematically document and describe a suite of enamel defects in the Xujiayao juvenile maxilla, an East Asian hominin from the early Late Pleistocene that may represent an unknown hominin lineage. In addition, we determine the chronology of growth disruptions represented by matched linear enamel hypoplasias, evaluate the long‐held hypothesis that the large brownish pit on the I¹ is evidence of dental fluorosis and assess the utility of micro‐computed tomography (micro‐CT) techniques in analyzing enamel defects. With the use of binocular microscopy, scanning electron microscopy (SEM) and micro‐CT imaging techniques, the Xujiayao teeth were found to exhibit a high number of pit‐form defects seldom seen in the fossil record. By matching the timing of linear enamel hyperplasia across multiple teeth, a minimum of five developmental disruptions were identified, indicating that the Xujiayao juvenile experienced several growth disturbances during its short lifespan. Our SEM and micro‐CT analyses suggest that the large pit on the I¹ is an enamel hypoplasia due to its morphology and pre‐eruptive enamel thinning. It is not a post‐eruptive fluorotic pit, and there is no evidence of chalkiness or opacity associated with dental fluorosis. The micro‐CT technique made it possible to verify the presence of enamel hypoplasia and to more precisely quantify defect dimensions, especially in unerupted teeth and shallow hypoplasias that are difficult to detect by binocular microscopy or SEM. Copyright © 2015 John Wiley & Sons, Ltd.