Preservation of DNA in ancient Egyptian mummies

The presence of DNA has been demonstrated in the cell nuclei of an ancient Egyptian mummy fragment. When extracted, this DNA proved to be degraded to a considerable extent and chemically modified. However, the preservation of nucleic acids in this specimen suggests that applying recombinant DNA techniques to the study of ancient mummified tissues might prove to be a fruitful future area of research.     

Mining sick: Creatively unsettling normative narratives about industry,environment, extraction,and the health geographies of rural,remote, northern,and Indigenous communities in British Columbia

Rural, remote, northern, and Indigenous communities on Turtle Island are routinely—as Cree Elder Willie Ermine says—pathologized. Social science and health scholarship, including scholarship by geographers, often constructs Indigenous human and physical geographies as unhealthy, diseased, vulnerable, and undergoing extraction. These constructions are not inaccurate: peoples and places beyond urban metropoles on Turtle Island live with higher burdens of poor health; Indigenous peoples face systemic violence and racism in colonial landscapes; rural, remote, northern, and Indigenous geographies are sites of industrial incursions; and many rural and remote geographies remain challenging for diverse Indigenous peoples. What, however, are the consequences of imagining and constructing people and places as “sick”? Constructions of “sick” geographies fulfill and extend settler (often European white) colonial narratives about othered geographies. Rural, remote, northern, and Indigenous geographies are discursively “mined” for narratives of sickness. This mining upholds a sense of health and wellness in southern, urban, Euro‐white‐settler imaginations. Drawing from multi‐year, relationship‐based, cross‐disciplinary qualitative community‐informed experiences, and anchored in feminist, anti‐colonial, and anti‐racist methodologies that guided creative and humanities‐informed stories, this paper concludes with different stories. It unsettles settler‐colonial powers reliant on constructing narratives about sickness in others and consequently reframes conversations about Indigenous well‐being and the environment.     

Micro-CT-assisted determination of the shattering and non-shattering phenotypes of charred rice spikelet bases from archaeological sites: a new methodological approach

The selection of a non-shattering phenotype is a pivotal change in the process of rice domestication. However, current research is heavily restricted by the preservation conditions of macro-plant remains in early and middle Neolithic sites, as very limited well-preserved rice spikelet bases could be retrieved. We present a non-destructive method based on micro-computed tomographic (CT) scanning, which could provide detailed visualization of the internal structures of charred spikelet bases and efficiently discriminate the shattering and non-shattering phenotypes of rice spikelet bases according to the abundance of fusiform echinate phytoliths. It could be widely applied in different contexts, especially those poorly preserved specimens and tempers in pottery sherds, greatly improving our knowledge of rice domestication.     

Breadfruit Fermentation in Pohnpei,Micronesia: Site Formation,Archaeological Visibility,and Interpretive Strategies

Fermentation is a common method of processing and preserving breadfruit throughout the Pacific Islands. While these pits are often reported in the archaeological record, they can pose some interpretive challenges. This paper presents an analysis of probable archaeological breadfruit (Artocarpus altilis (Parkinson) Fosberg) fermentation pits on Temwen Island, Pohnpei, Micronesia. Using the existing ethnographic record as a guide, I present archaeological and paleoethnobotanical criteria for identifying fermentation features. Architectural and stratigraphic analyses from four excavated pits match ethnographic expectations. Phytolith analysis shows some concurrence in terms of taxonomic data, but is more useful for indicating disturbance specific to building activities. Based on the distribution and size of pits present in the survey area, I suggest that breadfruit fermentation took place as primarily a household level (rather than community level) activity during the late prehistoric and early historic period in Pohnpei.     

出土青铜酒器残留物分析的尝试

利用快速溶剂萃取法,从出土青铜酒器内的土壤中提取有机残留物．对其作高效液相色谱定性分析,发现其中含有酒石酸,表明这些青铜酒器在下葬时可能盛着酒。快速溶剂萃取法的萃取剂为1％磷酸（体积比）溶液,较之传统有机溶剂,操作时更为方便和安全。此方法可推广于陶器中残留物的分析．具有较为广泛的应用前景。     

Ancient DNA in human bones from Neolithic and Bronze Age sites in Greece and Crete

Attempts were made to detect ancient DNA (aDNA) in samples of 88 human skeletons from eight Neolithic and Bronze Age sites in Greece and Crete. Ancient DNA was absent in specimens from Nea Nikomedia, Lerna, Karaviádena (Zakro), Antron Grave Circle A and Mycenae Grave Circle A. For each of three skeletons from Antron Grave Circle B that were sampled, polymerase chain reactions (PCRs) gave products for nuclear but not mitochondrial DNA, but amplicon yield was low and inconsistent with replicate PCRs failing to give reproducible results. With specimens from Mycenae Grave Circle B, evidence for mitochondrial aDNA was obtained for four of the 22 skeletons that were studied, and at Kouphovouno evidence for mitochondrial and/or nuclear aDNA was obtained with eight of the 20 skeletons that were examined. We conclude that, although aDNA might be present in some Eastern Mediterranean skeletons from later centuries of the Bronze Age, it is not commonly found in material from this period and is likely to be absent from older material.     

试论温峤

温峤墓是迄今为止南京地区发现的墓主身份明确、地位最高的东晋勋臣墓葬。本依据考古发现及献资料,对温峤家世、事迹和葬地进行比较全面的考察。     

The fish of Lake Titicaca: implications for archaeology and changing ecology through stable isotope analysis

Research on past human diets in the southern Lake Titicaca Basin has directed us to investigate the carbon and nitrogen stable isotopes of an important dietary element, fish. By completing a range of analyses on modern and archaeological fish remains, we contribute to two related issues regarding the application of stable isotope analysis of archaeological fish remains and in turn their place within human diet. The first issue is the potential carbon and nitrogen isotope values of prehistoric fish (and how these would impact human dietary isotopic data), and the second is the observed changes in the fish isotopes through time. Out of this work we provide quantitative isotope relationships between fish tissues with and without lipid extraction, and a qualitative analysis of the isotopic relationships between fish tissues, allowing archaeologists to understand these relationships and how these values can be applied in future research. We test a mathematical lipid normalization equation to examine whether future researchers will need to perform lipid extraction procedures for Lake Titicaca fish. We also analyze a number of aquatic plants to better understand the range of isotopic signatures of the Lake Titicaca ecosystem. We use these data to better understand prehistoric human diet and the role that fish may have played in the past as well as potential changes in local lake ecology through time.     

Subsistence practices and pottery use in Neolithic Jordan: molecular and isotopic evidence

This paper presents direct evidence of subsistence practices and pottery use at a Late Neolithic site at al-Basatîn, northern Jordan. Measurable concentrations of C_16:0 and C_18:0 were recovered from 8 of 10 archaeological pottery fragments through use of a microwave-assisted silica gel and aminopropyl solvent protocol developed for the isolation and concentration of free fatty acids in marine sediments. Subsequent isotopic analysis of the surviving C_16:0 and C_18:0 saturated fatty acids revealed ∂¹³C ratios consistent with those of adipose fats of ruminant and non-ruminant animals pastured on lands adjacent to the Jordan Valley. The high recovery of diagnostic compounds from the al-Basatîn material is discussed in context of a wider examination of the initial development and use of pottery in the Fertile Crescent, and the emerging debate concerning the efficacy of stable carbon isotope values in characterizing organic residues embedded in pottery fragments recovered from the earliest ceramic horizons in the Middle East and Europe.     

Towards the identification of dyestuffs in Early Iron Age Scandinavian peat bog textiles

A large systematic dye investigation of prehistoric Danish and Norwegian bog textiles was carried out using high performance liquid chromatography with photo diode array detection. After the selection of the most suitable protocol for dye extraction and HPLC analysis for this specific group of archaeological samples, the second part included the characterisation of the dyes detected in the whole series of the Early Iron Age textiles and the interpretation of the dyeing technology. Natural organic dyes were found from the three main categories of natural dyes, hence throwing new light on the use of biological dye sources in Early Iron Age Scandinavia. The results clearly indicate that most Scandinavian peat bog textiles originally were dyed and that already during the 1st millennium BC, the populations in Scandinavia were familiar with the dyeing technology.