The influence of different tempers on the composition of pottery

The modification of the raw clay by the potter to produce a paste suitable for the intended purposes adds a layer of obfuscation to the problem of provenancing the original clay source by chemical fingerprinting. By preparing different pastes from the same commercially available raw clay and their chemical analysis by Instrumental Neutron Activation Analysis, an experiment under controlled conditions (tempers, mixing ratios, firing temperatures and sampling methods) sheds light on the influence of different tempers.     

Enhancing Understanding of the Emergence of Global Trade: Analysis of 17th‐ to 19th‐Century Spanish Coins Recovered from Western Australian Shipwrecks Using Laser Ablation – Inductively Coupled Plasma – Mass Spectrometry (LA–ICP–MS)

This research uses legacy data from shipwrecks to further our understanding of global silver movement in the 17th to 19th centuries by analysing a collection of silver coins held by the Western Australian Museum. Three hundred and eighty‐nine silver coins were analysed for their trace element fingerprint in order to identify provenance. The coins are a selection from the ships Batavia, Vergulde Draeck, Zuytdorp, Rapid and Correio da Azia, all wrecked off the coast of Western Australia between 1629 and 1816. Analysis was undertaken using laser ablation – inductively coupled plasma – mass spectrometry (LA–ICP–MS), a relatively non‐destructive technique with a sensitivity of parts per million to parts per billion. Data were interpreted using linear discriminant analysis (LDA), which allowed the coins of known provenance to be sorted into identifiable subgroups on the basis of their trace and minor elemental fingerprints, while 27 unidentified coins were compared with this database and their mint of origin predicted. These results have implications for the provenance determination of archaeological artefacts of many materials.     

Seal dies from the National Museum of Slovenia: Non-destructive analyses of medieval and early modern copper alloys

A representative group of seal dies made of copper alloys from the collection of the National Museum of Slovenia was investigated by energy-dispersive X-ray fluorescence spectrometry (EDXRF). Chronologically, the artefacts date from the early 13th century to the present. Non-destructive EDXRF screening of elemental fingerprints of Cu, Zn, Pb, Ag, Sn and Sb was performed on 95 seal dies, and the obtained elemental data set was further processed by chemometric tools such as principal component analysis (PCA). The relationships between the elemental composition of analysed seal dies, type of alloy applied and their historical context—date, location of manufacture and, possibly, social rank of the owner—were examined in detail.     

Multi-isotope fingerprints (O,Sr and Pb) in archaeological animal bone bioapatite: Similarity search and the suitability for provenance analysis in a geologically complex Alpine region

Multi-isotope fingerprints in the bioapatite of archaeological skeletons are mostly superior over single isotope analyses for provenance studies. Gaussian mixture model (GMM) clustering is a novel tool for a similarity search among multidimensional data sets and at the same time permits the evaluation of the structural importance of particular isotopic ratios in the data set. We applied three GMM clustering experiments on multi-isotope fingerprints—stable strontium (Sr), lead (Pb) and oxygen (O) isotopic ratios—established in 217 archaeological animal bones excavated along a specific transect across the European Alps. This reference region had been in use since prehistoric times by humans who crossed the Alps from north to south, and vice versa. The resulting clusters permit a spatial assignment of the specimens with a very high probability, in particular with regard to the geological complexity of the region. A combination of Sr with Pb stable isotopes led to an optimal differentiation between the southern and northern Alpine forelands that cannot be distinguished from each other by ⁸⁷Sr/⁸⁶Sr ratios alone, while the contribution of δ¹⁸O is not particularly high. The isotopic mapping and subsequent cluster analysis is suitable for the analysis of archaeological human finds and the reconstruction of the direction of transalpine mobility and trade.     

A new miniSTR heptaplex system for genetic fingerprinting of ancient DNA from archaeological human bone

The analysis of short tandem repeats (STRs) is a useful tool in various contexts of ancient DNA research. Main applications are the reconstruction of kinship, identification, and authentication. Here we describe a short amplicon autosomal short tandem repeat (miniSTR) heptaplex system for the amplification of D13S317, D21S11, D18S51, TH01, D5S818, FGA and Amelogenin from highly degraded DNA as an inexpensive alternative to commercially available kits. All primers were newly designed and the amplicon length of all systems is less than 200 bp, with the exception of some rare alleles in the FGA and D21S11 systems. To validate the suitability of this system for typing STRs from human specimens with low DNA preservation we systematically tested it on 20 skeletal samples from four archaeological sites representing different burial environments and time spans since death. Finally, to test the sensitivity of the heptaplex system, we analyzed serial dilutions of control DNA and ancient DNA extracts. Using the system we were able to reproducibly obtain full STR profiles, down to a concentration of 0.06 ng DNA. Even with 0.004 ng DNA partial profiles could be amplified. The accumulated power of discrimination for the six selected STR loci is 0.99999984, plus the option of genetic sex determination through Amelogenin. The tests conducted prove that the system presented is efficient and especially suited for cases where STRs have to be typed and sex has to be assessed from human specimens with highly degraded DNA.