Molecular markers for the discrimination of Triticum turgidum L. subsp. dicoccum (Schrank ex Schübl.) Thell. and Triticum timopheevii (Zhuk.) Zhuk. subsp. timopheevii

The persistent uncertainty on the classification of the “new” glume wheat found in Neolithic and Bronze Age sites from Greece and other European settlements might be resolved only through analysis of its ancient DNA. Tools able to discriminate among different Triticum species on the basis of scarce, very damaged DNA, are therefore essential. While current attempts concentrate on DNA fragments sequencing and comparison, in some instances PCR-based selective amplification techniques might offer a cheaper and quicker alternative. The purpose of this research was therefore the identification of species-specific primers, able to distinguish caryopses of Triticum timopheevii subsp. timopheevii from those of Triticum turgidum subsp. dicoccum. Primers and their working conditions were defined and optimized using DNA from modern accessions. The ribosomal primers ITS1 tim and ITS2 tim, and the nuclear primer acetyl-coenzyme A tim clearly discriminated the sequences of Triticum timopheevii from other species. Finally, Neolithic charred wheat grains found in the sites of Sammardenchia (Pozzuolo del Friuli, Udine) and La Marmotta (Lago di Bracciano, Roma), belonging to the “new” wheat type or to emmer, were tested with the three selected primers. However, the results were not conclusive, because the samples analysed were apparently too degraded to yield useful DNA.     

Dysentery in the crusader kingdom of Jerusalem: an ELISA analysis of two medieval latrines in the City of Acre (Israel)

Dysentery was a significant cause of ill health during medieval times according to written sources of the period. However, it has been very difficult to detect the organisms that cause dysentery in archaeological samples from latrines and cesspools. This is because the cysts of the enteric parasites that cause dysentery are very small and usually fragment in the soil, in contrast to the robust ova of parasitic intestinal worms. This study uses a monoclonal enzyme-linked immunosorbent assay (ELISA) technique to identify the fragments of those enteric parasites that cause diarrhoea. Samples were taken from two excavated medieval latrines in the city of Acre. Coins and radiocarbon dating suggest that the latrines were in use during the thirteenth century CE. Positive results were noted for Entamoeba histolytica and Giardia duodenalis, while Cryptosporidium parvum was negative. This is the first time that either of these parasites have been identified in the Middle East using these methods.     

Six seasons of wild pea harvest in Israel: bearing on Near Eastern plant domestication

Harvest experiments of wild crop relatives are essential for our understanding of the biology of wild plants embodied in the economy of ancient forager communities. Most of the previous experimental harvest reports spanned a single season and therefore are unable to address questions concerning long-term yield potential. Herein we report on six consecutive harvest seasons taking place at four sites in Israel that harbour wild pea populations: three typical Pisum fulvum sites and one typical Pisum elatius site. Three out of the four sites showed no indication of a decline in grain yield as a result of repeated harvest and the removal of a considerable portion of mature seeds. Site-specific factors seem to have a strong influence on the grain yield as no seasonal yield similarity was observed between adjacent and ecologically similar sites harbouring the same wild pea taxon. The erratic year-to-year wild pea yield we observed calls for a reassessment of the presumed role of grain legume species in Near Eastern hunter–gatherers' diet. Combined with published data on cultivation of wild pea, our results are inconsistent with models suggesting protracted domestication of Near Eastern grain legumes or with the preliminary attempts to apply Niche Construction Theory to the study of plant domestication in the Near East.     

Genetic analysis of archaeological wood remains: first results and prospects

A total of 51 ancient oak wood samples originating from various European archaeological sites, dating from the Neolithic period to the 18th century, were assayed for the presence of reproducible chloroplast (cp) DNA sequences. Five polymorphic chloroplast fragments were targeted. Only five of the samples could be fully genetically characterised, revealing four different oak cpDNA haplotypes. In all cases, the haplotypes detected on ancient woods and the haplotypes characterised from fresh samples from the same localities matched. Overall, this congruence is consistent with a genetic continuity between ancient and modern European oaks, confirming the hypothesis that the mapped genetic patterns largely reflect the original structure that established during the post-glacial. This stability of the genetic structure implies that, in the future, the technique could be used to infer or confirm the transport of wood by man, providing interesting perspectives for the genetic analysis of ancient woods.     

Early and mid Holocene tool-use and processing of taro (Colocasia esculenta), yam (Dioscorea sp.) and other plants at Kuk Swamp in the highlands of Papua New Guinea

Recent multidisciplinary investigations document an independent emergence of agriculture at Kuk Swamp in the highlands of Papua New Guinea. In this paper we report preliminary usewear analysis and details of prehistoric use of stone tools for processing starchy food and other plants at Kuk Swamp. Morphological diagnostics for starch granules are reported for two potentially significant economic species, taro (Colocasia esculenta) and yam (Dioscorea sp.), following comparisons between prehistoric and botanical reference specimens. Usewear and residue analyses of starch granules indicate that both these species were processed on the wetland margin during the early and mid Holocene. We argue that processing of taro and yam commences by at least 10,200 calibrated years before present (cal BP), although the taro and yam starch granules do not permit us to distinguish between wild or cultivated forms. From at least 6950 to 6440 cal BP the processing of taro, yam and other plants indicates that they are likely to have been integrated into cultivation practices on the wetland edge.     

The first European representative of the South American family Xenopteraidae (Insecta: Megasecoptera) discovered in the Guadalupian of Lodève (France)

NEL, A., LAPEYRIE, J. & GARROUSTE, R. May 6 2019. The first European representative of the South American family Xenopteraidae (Insecta: Megasecoptera) discovered in the Guadalupian of Lodève (France). Alcheringa 43, 436–440. ISSN 0311-5518.

A new genus, Sinitshenkovae gen. nov., is described, comprising the Carboniferous species Sinitshenkovae hueneckeni (Pinto & Pinto de Ornellas, 1978 Pinto, I.D. & Pinto de Ornellas, L., 1978. Upper Carboniferous insects from Argentina. 1. Familia Diaphanopteridae (Megasecopteroidea). Pesquisas (Zoologiat) 10, 87–95. [Google Scholar]) comb. nov. from South America and new Guadalupian species S. gallica sp. nov. from France. These two species are attributed to the Paleozoic South American family Xenopteraidae, a previously monospecific family only containing Xenoptera riojaensis Pinto, 1986 Pinto, I.D., 1986. Carboniferous insects from Argentina. 3. Family Xenopteridae Pinto nov. fam. (Megasecoptera). Pesquisas (Zoologia) 18, 23–29. [Google Scholar]. Sinitshenkovae gallica sp. nov. is therefore the youngest representative of this family and the only record outside South America, which suggests that our current knowledge of the palaeobiogeography of Permian and Carboniferous insects remains incomplete.

André Nel [anel@mnhn.fr] and Romain Garrouste [garroust@mnhn.fr], Institut Systématique Evolution Biodiversité (ISYEB), Muséum national d’Histoire naturelle, CNRS, Sorbonne Université, EPHE, 57 rue Cuvier, CP 50, 75005 Paris, France; Jean Lapeyrie [jean.lapeyrie@orange.fr], Corniche de Fontbonne, F-34700 Lodève, France.     

Prehistoric behavioral depression of cormorant (Phalacrocorax spp.) on the northern California coast

Resource depression is often assumed to result from the over-exploitation of a prey species leading to declines in its population, and therefore availability, to human hunters. However, two other forms of resource depression are noted in the ecological literature: behavioral and micro-habitat depression. Data presented in this paper demonstrate resource depression of cormorants (Phalacrocorax spp.), a coastally breeding and semi-pelagic bird genus, at a coastal site in northern California. Citing modern cormorant behavior and a lack of evidence for intensive rookery exploitation, it is argued that the observed resource depression resulted from changes in cormorant behavior that decreased encounter rates for human hunters. Such behavioral depression is cited as a buffer to the extirpation of prey in mainland settings.     

Evidence for variability among squash seeds from the Hoxie site (11CK4), Illinois

Uncarbonized Cucurbita pepo L. (squash) seed coats were recovered from the base of a fortification ditch in water-saturated contexts at the Hoxie Farm site (11CK4) in northeastern Illinois. A direct date on one specimen indicated association with the Huber phase occupation, or about 1600 A.D. Sizes were highly variable, suggesting the presence of two or more squash varieties. To explore that possibility, length and width measurements were obtained for 21 specimens. A simple hierarchical cluster analysis revealed the presence of three distinct seed size groups. The two smaller conform to sizes displayed by modern varieties of subspecies ovifera which is the type domesticated in North America. Sizes for the third group of large seeds fall outside the range of any modern subspecies ovifera and within the range for the Mexican domesticate subspecies pepo. Similar large sized seeds first appear in the archaeological record from the Midwest during the Late Mississippian times. The potential for long-term prehistoric cultivation of two distinct lineages in the Midwest, and problems inherent in the scenario are discussed.     

A new species of Scirrotherium Edmund & Theodor, 1997 (Xenarthra,Cingulata, Pampatheriidae) from the late Miocene of South America

Góis, F., Scillato-Yané, G.J., Carlini, A.A. & Guilherme, E. 2013. A new species of Scirrotherium Edmund & Theodor, 1997 (Xenarthra, Cingulata, Pampatheriidae) from the late Miocene of South America. Alcheringa 37, 175–186. ISSN 0311-5518.

A new species of Scirrotherium Edmund & Theodor (Pampatheriidae) is described: S. carinatum. This genus was previously represented by a single species, S. hondaensis Edmund & Theodor, 1997, recorded from the middle Miocene (Laventan) of Colombia. Pampathere remains are common in the ‘Conglomerado osífero’ (late Miocene) of the Ituzaingó Formation in the Paraná River cliffs of Entre Ríos, Chubut (Argentina) and Acre (Brazil). All of them were referred to Kraglievichia paranense, and they are mostly osteoderms. However, only a few of these specimens are strictly consistent with that species. The new species described herein differs from S. hondaensis in having very thin osteoderms with more elaborate ornamentation. The movable band (or imbricate) osteoderms have a sculptured exposed surface and a single transverse row of anterior foramina; the lateral margins are wider and also bear foramina, and the longitudinal central elevation is long and proximally wide, and forms a posteriorly raised crest. This elevation is delimited by a fairly broad and shallow depression on each side, separating the marginal elevation from the longitudinal central one. The exposed surfaces of fixed (or fused) osteoderms bear large and deep anterior foramina, the longitudinal central elevation and delimiting depressions are very pronounced. This new species increases the known diversity of the pampatheres from the ‘Conglomerado osífero’. Additionally, a new nomenclatural scheme for Pampatheriidae osteoderms is proposed.

Flávio Góis [goisf@fcnym]unlp.edu.ar], Gustavo Juan Scillato-Yané [scillato@fcnym.unlp.edu.ar], Alfredo Armando Carlini [acarlini@fcnym.unlp.edu.ar], Departamento Científico Paleontología Vertebrados, Museo de La Plata, Paseo del Bosque, s/n, 1900, La Plata, Buenos Aires, Argentina and CONICET; Edson Guilherme [guilherme@ufac.br], Universidade Federal do Acre, Laboratório de Pesquisas Paleontológicas (LPP), BR 364, Km 04, 69.915-900. Rio Branco, AC, Brazil. Received 11.5.2012; revised 31.7.2012; accepted 4.9.2012 .