Alkaline cooking and stable isotope tissue-diet spacing in swine: archaeological implications

In this study we examine the effects of alkaline cooking on carbon and oxygen stable isotopic ratios of mineralized tissues from nine pigs raised on monotonous mixed C3/C4 vegetarian diets. Two sources of collagen (humerus and mandible) and two sources of apatite (humerus and enamel) were analyzed. Within each diet group, humerus and mandible collagens were found to record equivalent δ¹³C and δ¹⁸O ratios; however, enamel apatite was found to be enriched over bone apatite by 2.3‰ in carbon and 1.7‰ in oxygen. Alkaline cooking was found to slightly, but significantly increase the Δ¹³C_{collagen-diet} and Δ¹⁸O_{collagen-diet} of bone collagen. A similar trend towards enrichment was observed in bone and enamel Δ¹³C_apatite-diet and Δ¹⁸O _apatite-diet, but the differences were not significant. Observed isotopic shifts were consistent with increased nutrient utilization of the alkaline-cooked maize as compared to raw maize. In addition, a reexamination of the relationship between diet and tissue carbon isotopic values suggests that species and alimentary type should be considered when interpreting ancient diets.     

Stone-boiling maize with limestone: experimental results and implications for nutrition among SE Utah preceramic groups

Groups living on Cedar Mesa, SE Utah in the late Basketmaker II period (Grand Gulch phase, AD 200–400) were heavily maize-dependent, but lacked beans as a supplemental plant protein, and pottery vessels for cooking. Common occurrence of limestone fragments in their household middens suggests 1) limestone may have been used as the heating element for stone-boiling maize and 2) this practice might have made some maize proteins more available for human nutrition. Experiments examined these possibilities; results indicate that stone-boiling with Cedar Mesa limestone creates an alkaline cooking environment suitable for nixtamalization of maize kernels, and that maize cooked in this fashion shows significant increases in availability of lysine, tryptophan, and methionine. Archaeological limestone fragments from a Grand Gulch phase site show amounts of fragmentation and changes in density consistent with repeated heating. While not conclusive, these data indicate that further research (e.g., examination of archaeological limestone fragments for maize starch grains or phytoliths) is warranted. It is suggested that greater attention be paid to archaeological indications of stone-boiling with limestone among maize-dependent but pre-pottery societies.