Molecular markers for the discrimination of Triticum turgidum L. subsp. dicoccum (Schrank ex Schübl.) Thell. and Triticum timopheevii (Zhuk.) Zhuk. subsp. timopheevii

The persistent uncertainty on the classification of the “new” glume wheat found in Neolithic and Bronze Age sites from Greece and other European settlements might be resolved only through analysis of its ancient DNA. Tools able to discriminate among different Triticum species on the basis of scarce, very damaged DNA, are therefore essential. While current attempts concentrate on DNA fragments sequencing and comparison, in some instances PCR-based selective amplification techniques might offer a cheaper and quicker alternative. The purpose of this research was therefore the identification of species-specific primers, able to distinguish caryopses of Triticum timopheevii subsp. timopheevii from those of Triticum turgidum subsp. dicoccum. Primers and their working conditions were defined and optimized using DNA from modern accessions. The ribosomal primers ITS1 tim and ITS2 tim, and the nuclear primer acetyl-coenzyme A tim clearly discriminated the sequences of Triticum timopheevii from other species. Finally, Neolithic charred wheat grains found in the sites of Sammardenchia (Pozzuolo del Friuli, Udine) and La Marmotta (Lago di Bracciano, Roma), belonging to the “new” wheat type or to emmer, were tested with the three selected primers. However, the results were not conclusive, because the samples analysed were apparently too degraded to yield useful DNA.     

‘Come Buy,Come Buy’: Christina Rossetti's ‘Goblin Market’ and the Cries of London

Placing Christina Rossetti's ‘Goblin Market’ (1862) within the rich context of children's London guidebooks establishes a literary tradition in which readers are counseled to be wise urban consumers. During this period of rapid industrialization, exotic foods appeared on English tables amid public fears about food adulteration. Rossetti's poem and contemporary guidebooks transmit the anxiety surrounding food selection and consumption. From Aunt Busy-Bee's New London Cries (1852) to Aunt Louisa's London Alphabet (1872), London is depicted as an urban sounding board for commercial opportunities and moral choices. Removed from agrarian sites of production, urban readers are urged to select food according to the moral character of the vendor. In Rossetti's famous poem, Lizzie's and Laura's choices are immediately relevant to readers who hearkened to similar cries shouted daily in their neighborhoods. Rossetti herself would have heard them growing up in London, and these traditional cries bear semantic and metaphoric similarities to the goblins' cries. Apart from serving as inspirations for Rossetti's work, guides to the cries are also important documents in their own right, part of the corpus of near-forgotten work penned for children to amuse them in their idle hours as well as to instruct them in proper practices of commercial enterprise and moral consumption. The child reader is invited to know London and to absorb lessons by piecing together the letters and sounds of the metropolis.     

An improved PCR method for endogenous DNA retrieval in contaminated Neandertal samples based on the use of blocking primers

Neandertal skeletal remains are usually contaminated with modern human DNA derived from handling and washing of the specimens during excavation. Despite the fact that the distinct Neandertal haplotypes allow the design of specific primer pairs, for instance in most of the mitochondrial DNA (mtDNA) hypervariable region 1 (HVR1), the human contaminants can often outnumber the endogenous DNA, thus preventing a successful retrieval of Neandertal sequences. We have developed a novel PCR method, based on the use of blocking primers that preferentially bind to modern human contaminant DNA and block their amplification, and greatly improve the efficiency of Neandertal DNA retrieval. We tested the method in four El Sidrón Neandertal samples (two teeth and two bone fragments) with different contamination levels and taphonomic conditions, and we have been able to significantly increase the Neandertal yield from figures around 25.23% (5–69.6%) up to 90.18% (75.3–100%).     

西藏博物馆馆藏板结藏经《松赞干布遗训》的修复

《松赞干布遗训》为西藏博物馆藏一件15世纪梵夹装手抄本,入藏前曾遭水侵致整体板结为四沓。文章描述了这件板结藏经的装帧、纸张等物质特征及破损状况,并详细介绍了所采取的分揭、清洁、补缺、加固、平整等修复处理方法。修复过程中根据藏经文物的具体特征,在一般修复方法基础之上对操作细节进行了设计和调整,包括借助SYMPATEX进行润潮、使用超细纤维布进行清洁、染纸时对湿纸进行揉搓、将刷糨后的补纸预先晾至半干,以及对补处和经叶进行差异性润潮等。修复工作取得了较为满意的结果,相关经验能够为同类文物的保护修复提供一定参考。