The canopic jars of Rameses II: real use revealed by molecular study of organic residues

Four blue-glazed faience jars, on exhibition in the Louvre Museum, are referred to as the canopic jars of Rameses II. Their recent typological re-examination rendered this original assignment questionable. In order to clearly determine their use, a study of two successive organic residues from the canopic jars, using molecular biomarkers analysed by GC-MS and LC-MS and absolute dating by ¹⁴C, was initiated. The results revealed that these two materials were not contemporary to the reign of Rameses II. The first one, scraped from the interior face of one jar, was identified as an unguent made of coniferous oil and animal fat and dated from the Third Intermediate Period. The second one, originally stored with glued linen inside one jar, is likely an embalming substance, made of pure vegetable resin (Pistacia) and dated from the Ptolemaic Period. These results clarify a controversy which has been lasting over a century. The famous blue-glazed faience jars are not the canopic jars of Rameses II but are confirmed as situlae which were reused at least two times: first to store unguents during the Intermediate Period and later to store embalming packages of an unknown person during the Ptolemaic period.     

Essentials in the use of mycolic acid biomarkers for tuberculosis detection: response to “High-throughput mass spectrometric analysis of 1400-year-old mycolic acids as biomarkers for ancient tuberculosis infection” by

High molecular weight long-chain mycolic acids are key structural components of the cell envelope of Mycobacterium tuberculosis and they are established as biomarkers for the identification of both ancient and modern tuberculosis. Mycolic acids from M. tuberculosis have a characteristic profile, reflecting contributions from five major distinct homologous series of mycolate structural types. Diagnosis of tuberculosis in archaeological material, using mycolic acid biomarkers, depends on objective recognition of the key characteristic mycolic acid components. A recent article in this journal claimed that tuberculosis could be confirmed in ancient bones by high throughput mass spectrometric analysis of mycolic acids. Scrutiny of the data presented reveals no convincing evidence for the presence of mycolic acids, characteristic of the M. tuberculosis complex, in the skeletal remains examined. This communication reviews the essential criteria necessary for positive tuberculosis diagnosis, using mycolic acids.     

Faeces deposition on Amazonian Anthrosols as assessed from 5β-stanols

In the Amazon Basin, within a landscape of infertile soils, fertile Anthrosols of pre-Columbian origin occur (Amazonian Dark Earths or terra preta de Índio). These soils are characterized by high amounts of charred organic matter (black carbon, biochar) and high nutrient stocks. Frequently, they were considered as sign for intensive landscape domestication by way of sedentary agriculture and as sign for large settlements in pre-Columbian Amazonia. Beyond the archaeological interest in Amazonian Dark Earths, they increasingly receive attention because it is assumed that they could serve as a model for sustainable agriculture in the humid tropics (terra preta nova). Both questions lack information about the pre-Columbian practices which were responsible for the genesis of Amazonian Dark Earths. It has often been hypothesized that deposition of faeces could have contributed to the high nutrient stocks in these soils, but no study has focussed on this question yet. We analyzed the biomarkers for faeces 5β-stanols as well as their precursors and their 5α-isomers in Amazonian Dark Earths and reference soils to investigate the input of faeces into Amazonian Dark Earths. Using Amazonian Dark Earths as example, we discuss the application of threshold values for specific stanols to evaluate faeces deposition in archaeological soils and demonstrate an alternative approach which is based on a comparison of the concentration patterns of 5β-stanols with the concentration patterns of their precursors and their 5α-isomers as well as with local backgrounds. The concentration patterns of sterols show that faeces were deposited on Amazonian Dark Earths.     

Beyond the buildings: Formation processes of ancient Maya houselots and methods for the study of non-architectural space

The success of activity areas research in domestic contexts has highlighted the need for archaeologists working in the tropics to explore both indoor and outdoor spaces. The preservation of houselot boundaries at ancient Chunchucmil, Yucatán, Mexico, provides an ideal environment to explore methods for the investigation of broad spaces beyond buildings, to test ethnoarchaeological models of activity areas and refuse disposal, and to demonstrate the importance of open spaces to understanding ancient economic organization. The results of methodological experiments reported in this paper favor the use of systematic subsurface sampling (as opposed to surface collections) for characterizing specific uses of space around houses. Formation processes dictate that soil chemistry, paleoethnobotany, and other laboratory techniques should supplement excavations. Our explorations of three houselots suggest that ethnoarchaeological models of the use of space in contemporary houselots serve as successful templates for interpreting the use of space in ancient times. Post-abandonment deposition within houselots, however, can confuse these interpretations. This paper therefore reports a new ethnoarchaeological study that helps illuminate the nature of post-abandonment discard. After resolving critical issues of methodology and formation processes, this paper assesses the economic contribution of houselot activities. Given Chunchucmil’s agricultural marginality, such home economics played an important role in the subsistence needs of this urban center.     

Molecular evidence for the mixing of Meat,Fish and Vegetables in Anglo‐Saxon coarseware from Hamwic,UK

Absorbed lipid residues from 24 seventh‐ to ninth‐century coarseware potsherds from the major Anglo‐Saxon trading centre of Hamwic (Southampton, UK) were analysed by gas chromatography – mass spectrometry (GC–MS) in order to reconstruct the dietary habits of its population. The results show that the vessels were used for preparing ruminant fats and leafy vegetables. In addition, evidence was found for a minor contribution of aquatic foods. Beeswax was found once and most probably relates to a sealing function or to honey. Remarkable features were: (i) the isomeric mixture of octadecenoic acid (C_18:1 Δ^{7,8,9,10,11,12,13,14,15,16}) and 8‐ to 16‐hydroxyoctadecanoic acid, and (ii) the co‐occurrence of C_17:1, C_19:1 and isoprenoid fatty acids. These features were proposed as biomarkers for ruminant and aquatic food sources, respectively. Furthermore, the carbonyl position distribution in mid‐chain ketones was used to identify mixtures of animal‐ and plant‐derived ketones. The paper highlights the difficulty in interpreting complex lipid signatures that show a mixture of various foods, as observed in the majority of the samples. This was linked to the preparation of stews or the recycling of vessels. The results are considered alongside ceramic usewear data and existing data relating to environmental remains recovered from the Hamwic excavations.     

Potential vegetation markers – analytical pyrolysis of modern plant species representative of Neolithic SE Spain

A selection of plant species that may have been relevant for the Neolithic in the SW Mediterranean have been characterised with pyrolysis gas chromatography mass spectrometry (pyrolysis–GC/MS) in search for molecular vegetation markers. Roots and aerial parts were analysed separately for the following species: Anthyllis cytisoides, Asphodelus cerasiferus, Brachypodium retusum, Cistus albidus, Cistus clusii, Dorycnium pentaphyllum, Ephedra fragilis, Juniperus oxycedrus, Juniperus phoenicea, Olea europaea, Phillyrea angustifolia, Pinus halepensis, Pistacia lentiscus, Quercus coccifera, Rhamnus lycioides, Rosmarinus officinalis, Smilax aspera and Stipa tenacissima; furthermore domesticated plants were analysed, including wheat (Triticum aestivum, Triticum dicoccum, Triticum monococcum, Triticum timopheevi and Triticum turgidum), barley (Hordeum vulgare and H. vulgare Hulled) and legumes (Lathyrus cicera, Lathyrus sativus, Lens culinaris, Pisum sativa, Vicia ervilia, Vicia faba and Vicia sativa). This resulted in 290 potential markers. In addition, the organic matter of surface soils under different vegetation cover has been analysed to test the presence of the potential markers in the soil. Forty-six of the potential markers were detected in the soil organic matter, of which part have not been reported before. The results may be useful for interpretation of the organic matter composition of soils and plant remains, which can be valuable in archaeology.     

Identification of activity area signatures in a reconstructed Iron Age house by combining element and lipid analyses of sediments

One promising analytical method used in household archaeology in addition to inorganic (element) geochemical analysis is that of organic (lipid) analysis applied to anthropogenic sediments. We use both methods here to review chemical imprints that might be useful for recognizing space use and identifying daily activities in a reconstructed Iron Age village at Lejre, Denmark. As documented in many previous studies, element analysis enabled separate activity areas to be distinguished, but the results could not be used to identify the specific activities pursued in the areas. A more qualitative identification of activity areas was possible through lipid analysis, however. The carbon chain distribution, studied for Average Chain Length (ACL), Carbon chain Diversity Index (CDI) and Carbon Preference Index (CPI), enabled a similar separation to be achieved as by element analysis, so that the same areas could be discerned in addition to the reference samples. The stable was distinguished by a substantial input of coprostanol and even more so by 24-ethylcoprostanol, indicating a faecal input from herbivores. Trace levels of these markers were also identified at the entrance, where the animals had passed through. The dwelling area, consisting of two adjacent rooms, could be identified by the sterol ratio (cholesterol/[stigmasterol + β-sitosterol + campesterol]). Lipids from an archaeological context have decayed further toward simpler compounds and become more difficult to identify. Some markers have however a better potential for survival. The results emphasize the importance of further studies on ethnoarchaeological material in order to recognize past activities by element analysis. Moreover, the combination of element and lipid analyses provided a tool that enabled all the separate areas to be identified and provided positive identification of the activities concerned in all areas except the smithy.     

Organic Geochemical Evidence for Pine Tar Production in Middle Eastern Sweden During the Roman Iron Age

Several funnel-shaped features of unknown function were discovered at excavations related to a new stretch of the highway E4 in middle Sweden during 2002–2003. These features could be sub classed into two categories: large funnel-shaped pits dated to 600–1100 AD (Vendel period – Viking Age) and small funnel-shaped pits dated to 240–540 AD (late Roman Iron Age – Migration period) respectively. Soil samples were analysed for diterpenoids derived from abietic acid (mainly retene, abietic acid, dehydroabietic acid and methyl dehydroabietate) by Gas Chromatography-Mass Spectrometry (GC-MS) in order to test the hypothesis that the features might be connected to pine tar manufacture. For comparison, samples from historically known pine tar and charcoal production features were analysed. The resinous fraction in the larger funnel-shaped features were very similar to those of the historical pine tar and charcoal production features, while the composition in the small funnel-shaped pits was dominated by retene and methyl dehydroabietate. The biomarker, methyl dehydroabietate, could be identified in several of the soil samples from the funnel-shaped pits. We suggest that both of these features have been used for pine tar production, which makes the smaller funnel-shaped features the oldest known tar production features in Europe.