Phylogeographic analysis of barley DNA as evidence for the spread of Neolithic agriculture through Europe

Results of analyses of the photoperiod response gene (PPD-H1) and simple sequence repeats (SSRs) in modern landraces of cultivated barley were used as evidence for the mechanism of agricultural spread in Neolithic Europe. In particular, we explored the usefulness of considering adaptive genes as indicators of past selective pressures acting on crops, during their spread through Europe. In some areas, such as the Alpine region, Britain and Scandinavia, we have evidence to suggest that the adaptation of crops to certain climatic conditions may have contributed to the timing of agricultural spread. At the northern fringes of Europe, and in higher altitude locations in central Europe, the introduction of more suitably adapted cereals may have facilitated successful agriculture to trigger agricultural expansion. This research opens up the possibility of investigating other genetic adaptations to climate, which would permit a fuller evaluation of the relative contributions of climate/crop and forager/farmer interactions in the process of agricultural spread.     

Next generation sequencing of DNA in 3300-year-old charred cereal grains

We investigated whether ‘next generation’ methods can be used to sequence ancient DNA molecules in charred cereal grains. We prepared a DNA extract from a mixed sample of barley, einkorn, emmer and broomcorn millet, taken from a 3300-year-old assemblage of charred cereal grains from Assiros Toumba, Greece. Using the SOLiD 5500 system, we obtained 21,112,844 unique sequence reads. Of these, 178,779 had a 75% or greater nucleotide sequence similarity with one or more entries in the full nucleotide sequence database; 496 of these matches were to previously reported sequences from barley, einkorn, emmer, broomcorn millet or related species such as hexaploid wheat. The unique reads were also compared with a database comprising only wheat sequences. This analysis identified 1658 charred grain sequences that had 90% or greater similarity with segments of the wheat genome. The presence of barley, wheat and millet sequences in the next generation dataset confirms the presence of ancient DNA in this charred grain assemblage. Enrichment of extracts by hybridization capture or equivalent methods is likely to enable sequences to be obtained for entire genes and other genomic regions of interest.     

Latitudinal variation in a photoperiod response gene in European barley: insight into the dynamics of agricultural spread from ‘historic’ specimens

Between ca. 6000 BC and ca. 500 BC, barley cultivation spread across the continent of Europe from the extreme south to the extreme north. Carbon-dating would suggest that this spread, and indeed the spread of crop cultivation generally, varied in its pace, with ‘delays’ at certain points along its route. Such delays in the spread of agriculture have been explained as resulting from the slow assimilation of agricultural practices by existing indigenous human populations or as the time taken for the crops to adapt to novel climatic conditions, such as altered temperature regimes and day-lengths. A mutant form of the photoperiod response gene, Ppd-H1, causes barley to be non-responsive to long days, while the wild-type responsive form allows plants to flower in response to long days. We sequenced this gene in 65 ‘historic’ barley accessions, from the late 19th and early 20th centuries, in order to explore the potential role of environmental adaptation in the spread of agriculture. We chose to use ‘historic’ material, to complement the richer patterns in extant genetic lines, by spreading the data range in both time and space. Our ‘historic’ barley data shows a latitudinal divide in the Ppd-H1 gene similar to that found in extant lines, but with clearer geographical resolution, and extending northwards into the Arctic Circle. We discuss the implications of our results in relation to the dynamics of agricultural spread across Europe.     

Barley and identity in the Spanish colonial Audiencia of Quito: Archaeobotany of the 18th century San Blas neighborhood in Riobamba

Archaeological excavation of two late-18th century household middens in the San Blas parish of the colonial city of Riobamba resulted in the recovery of samples of archaeobotanical remains dating to the period before the AD 1797 destruction of the city in an earthquake. These seed remains were dominated by barley (Hordeum vulgare) and quinoa (Chenopodium quinoa). The dominance of these two grains in samples from artisans’ households in this marginal neighborhood suggests that we should question the assumption that imported Old World domesticates were associated with more elite houses during the Spanish colonial period in the Andes.     

Cropmarks in main field crops enable the identification of a wide spectrum of buried features on archaeological sites in Central Europe

Buried (syn. sunken, sub-surface and sub-soil) archaeological features on arable land can frequently be discovered due to visually detectable changes in crop growth termed cropmarks. The aim of this paper was to demonstrate the range of features identified through cropmarks on aerial photographs in stands of main field crops in the Czech Republic.     

What goes in does not always come out: The impact of the ruminant digestive system of sheep on plant material,and its importance for the interpretation of dung-derived archaeobotanical assemblages

Abstract

On archaeological sites where livestock dung was a major fuel source, plant material that survives digestion intact may well be preserved in the remnants of dung-fuelled fires. Preserved plant remains which were derived from dung relate to the diet of animals, and thus provide a way of investigating the agro-pastoral economies of the past. In order to improve our understanding of the taphonomic processes to which plant material is exposed to during digestion, we applied archaeobotanical methods to the analysis of dung from sheep fed a known diet of cereal and wild plant material. Two clear patterns emerge from these investigations. First, cereal material (grain or chaff) survives digestion poorly and was rarely found in the dung analysed. Second, large proportions of seeds of various wild species survive digestion in an identifiable form, probably due to their small size and/or protective coating. These findings are crucial for reliable interpretation of dung-derived plant material in archaeological settings.     

Wood exploitation and food supply at the border of the Roman Empire: the case of the vicus of Thamusida – Sidi Ali ben Ahmed (Morocco)

This paper presents the results of inter-disciplinary work drawing on archaeobotanical and archaeometric studies to trace the agroforestry landscape and the supply economy at the vicus of Thamusida in north-west Morocco at the border of the Roman Empire. The available data indicate the self-sufficiency of the settlement in both forestry and agricultural products throughout the period investigated from the end of the 1st century BC to the beginning of the 4th century AD. Charcoal data testify to the presence of a Quercus suber forest in the close surroundings of the site and its exploitation for a variety of forestry products such as timber, fuelwood, cork, and probably also leaves and acorns to feed livestock. The overwhelming presence of Q. suber in the archaeological layer investigated clearly indicates that this forest was under human influence prior to Roman occupation and was already partially degraded. Charred seed and fruit remains suggest that the diet of both troops and civilians was mainly based on locally grown products and that all the inhabitants of the site had access to good cereals such as barley, naked wheats and pulses with large seeds such as horse bean and pea; quality fruits, such as olive and grape, were also produced locally for fresh consumption. Organic residue analyses of the contents of ceramic vessels and plastered vats allowed archaeobotanical data to be complemented, thereby shedding light on some of the imports at Thamusida. Despite the remote location of this settlement, imported goods such as oil and wine were transported here in amphorae from different parts of the Empire.