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Gary A. King M. Thomas P. Gilbert Eske Willerslev Matthew J. Collins Harry Kenward 《Journal of archaeological science》2009
We report the recovery of short fragments of PCR amplifiable ancient DNA from exoskeletal fragments of the grain weevil Sitophilus granarius (L.) extracted from Roman and medieval deposits in Northern England. If DNA preservation in archaeological insect remains is widespread then many applications in the spheres of evolutionary studies and archaeology can be conceived, some of which are outlined. 相似文献
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M. T. P. Gilbert A. J. Hansen E. Willerslev G. Turner‐Walker M. Collins 《International Journal of Osteoarchaeology》2006,16(2):156-164
A principal problem facing human DNA studies that use old and degraded remains is contamination from other sources of human DNA. In this study we have attempted to contaminate deliberately bones and teeth sampled from a medieval collection excavated in Trondheim, Norway, in order to investigate this poorly understood phenomenon. Five pairs of teeth and bone samples were bathed in water containing various concentrations (from 10−9 and 10−21 g/l) of purified ΦX174 DNA. Subsequently the samples were subjected to a routine decontamination protocol involving a bleach bath followed by exposure to λ=254 nm ultraviolet light, prior to DNA extraction and analysis for evidence of the persistence of the contaminant. The results support previous speculation that bone is more susceptible to water‐borne sources of contaminant DNA, although both bone and teeth are readily contaminated and are difficult to decontaminate using the tested protocol. We believe that this is largely due to the porous nature of bone and teeth facilitating the deep penetration of the contaminant DNA. To simulate a more realistic handling situation, 27 further teeth were directly handled and washed, then decontaminated, prior to assaying for the residual presence of the handler's DNA. Surprisingly, although our results suggest that a large proportion of the teeth were contaminated with multiple sources of human DNA prior to our investigation, we were unable to contaminate the samples with further human DNA. One potential explanation may be the deposition of sediment or other structural changes that occur within the samples as they desiccate post‐excavation, which may protect samples from subsequent contamination, but also prevent the efficacy of bleach baths in decontaminating specimens. Copyright © 2006 John Wiley & Sons, Ltd. 相似文献
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Luise Ørsted Brandt Lena Diana Tranekjer Ulla Mannering Maj Ringgaard Karin Margarita Frei Eske Willerslev Margarita Gleba M. Thomas P. Gilbert 《Archaeological and Anthropological Sciences》2011,3(2):209-221
The use of wool derived from sheep (Ovis aries) hair shafts is widespread in ancient and historic textiles. Given that hair can represent a valuable source of ancient DNA,
wool may represent a valuable genetic archive for studies on the domestication of the sheep. However, both the quality and
content of DNA in hair shafts are known to vary, and it is possible that common treatments of wool such as dyeing may negatively
impact the DNA. Using quantitative real-time polymerase chain reaction (PCR), we demonstrate that in general, short fragments
of both mitochondrial and single-copy nuclear DNA can be PCR-amplified from wool derived from a variety of breeds, regardless
of the body location or natural pigmentation. Furthermore, although DNA can be PCR-amplified from wool dyed with one of four
common plant dyes (tansy, woad, madder, weld), the use of mordants such as alum or iron leads to considerable DNA degradation.
Lastly, we demonstrate that mtDNA at least can be PCR-amplified, cloned and sequenced from a range of archaeological and historic
Danish, Flemmish and Greenlandic wool textile samples. In summary, our data suggest that wool offers a promising source for
future ancient mitochondrial DNA studies. 相似文献
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Elena Gigli Morten Rasmussen Sergi Civit Antonio Rosas Marco de la Rasilla Javier Fortea M. Thomas P. Gilbert Eske Willerslev Carles Lalueza-Fox 《Journal of archaeological science》2009,36(12):2676-2679
Neandertal skeletal remains are usually contaminated with modern human DNA derived from handling and washing of the specimens during excavation. Despite the fact that the distinct Neandertal haplotypes allow the design of specific primer pairs, for instance in most of the mitochondrial DNA (mtDNA) hypervariable region 1 (HVR1), the human contaminants can often outnumber the endogenous DNA, thus preventing a successful retrieval of Neandertal sequences. We have developed a novel PCR method, based on the use of blocking primers that preferentially bind to modern human contaminant DNA and block their amplification, and greatly improve the efficiency of Neandertal DNA retrieval. We tested the method in four El Sidrón Neandertal samples (two teeth and two bone fragments) with different contamination levels and taphonomic conditions, and we have been able to significantly increase the Neandertal yield from figures around 25.23% (5–69.6%) up to 90.18% (75.3–100%). 相似文献
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