Ancient DNA in human bones from Neolithic and Bronze Age sites in Greece and Crete

Attempts were made to detect ancient DNA (aDNA) in samples of 88 human skeletons from eight Neolithic and Bronze Age sites in Greece and Crete. Ancient DNA was absent in specimens from Nea Nikomedia, Lerna, Karaviádena (Zakro), Antron Grave Circle A and Mycenae Grave Circle A. For each of three skeletons from Antron Grave Circle B that were sampled, polymerase chain reactions (PCRs) gave products for nuclear but not mitochondrial DNA, but amplicon yield was low and inconsistent with replicate PCRs failing to give reproducible results. With specimens from Mycenae Grave Circle B, evidence for mitochondrial aDNA was obtained for four of the 22 skeletons that were studied, and at Kouphovouno evidence for mitochondrial and/or nuclear aDNA was obtained with eight of the 20 skeletons that were examined. We conclude that, although aDNA might be present in some Eastern Mediterranean skeletons from later centuries of the Bronze Age, it is not commonly found in material from this period and is likely to be absent from older material.     

Ancient DNA typing shows that a Bronze Age mummy is a composite of different skeletons

Excavations at Cladh Hallan, a Bronze Age-Iron Age settlement on South Uist in the Outer Hebrides off the west coast of Scotland, revealed the skeletons of two adults, a sub-adult and a child buried beneath the foundations of three roundhouses. Osteological and isotopic evidence has shown that the male adult skeleton is a composite made up of parts of at least three different individuals. To test the hypothesis that the female skeleton was also a composite we examined ancient DNA from four of its components: the skull, mandible, right humerus and right femur. Seven polymerase chain reactions (PCRs) were attempted, these covering positions 15,996–16,420, 16–132 and 232–368 of the mitochondrial DNA hypervariable I and II regions. Three PCRs were successful for each sample and a total of 55 sequences were obtained from the cloned products. After exclusion of possible contaminating sequences, the remaining 34 were compared. It was concluded that the mandible, humerus and femur come from different individuals. Insufficient data were obtained to draw conclusions regarding the origin of the skull. The presence of two composite skeletons at Cladh Hallan indicates that the merging of identities may have been a deliberate act, perhaps designed to amalgamate different ancestries into a single lineage.     

Variable nucleotide tandem repeat (VNTR) typing of two palaeopathological cases of lepromatous leprosy from Mediaeval England

Using ancient DNA methods, we have examined in detail two archaeological cases of leprosy from Mediaeval England. The first was a child skeleton with rhino-maxillary changes typical of lepromatous leprosy (LL). The second case was the skeleton of a male adult who showed both typical rhino-maxillary changes and osteitis/periostitis on the leg and foot bones. Bone powder was sampled from both cases and DNA extracts were prepared. These were subjected to a series of polymerase chain reactions (PCRs) specific for regions on the Mycobacterium leprae genome. The repetitive element RLEP was used for confirmation of M. leprae DNA and then three polymorphic regions were successfully amplified and sequenced to determine the number of variable nucleotide tandem repeats (vntr) at these loci. These were the microsatellite regions ML2344 and ML2172 and the minisatellite region ML0058. Genotyping data from the strains preserved within the skeletal remains were compared with those obtained for a reference strain of M. leprae. Variation at these three loci was found between both burials and the reference strain, indicating that vntr typing of LL cases from the archaeological record is a useful way of confirming disease and an additional means of authenticating aDNA data. This demonstrates the feasibility of targeting multiple loci for phylogenetic studies of leprosy strains from archival sources.     

In Cloth We Trust

In this essay, I contextualize recent studies of cloth and culture in Africa, South America, and Oceania within changing anthropological concerns. Examining cloth as an important aspect of art, technology, and economy, I question how specific properties of objects are connected with cultural and social significance. Over the last two decades in particular, cloth—whether woven or nonwoven—has often been construed as an especially potent polyvalent symbol. Examining how scholars have explored this potential, I address the conceptual relocation of cloth in different, often contradictory regimes of value that join discrete physical locations, from isolated community to cosmopolitan museum.     

Deficiencies and challenges in the study of ancient tuberculosis DNA

We explore the standards of research and reporting needed to justify the destructive analysis of archaeological human bone for biomolecular studies of ancient tuberculosis (TB). Acceptable standards in osteological interpretation have been met in some biomolecular papers, but there are also cases where insufficient care has been taken in distinguishing between pathognomonic lesions and those that are ‘consistent with’ a diagnosis of TB. Some biomolecular studies have failed to recognize that archaeological bones might be contaminated with environmental mycobacteria whose DNA could give rise to false positives in polymerase chain reactions directed at members of the Mycobacterium tuberculosis complex. The difficulties of applying spoligotyping to ancient DNA have also been underestimated and conclusions drawn from such analyses are often weakly supported. Assumptions that mycobacterial DNA preserves better than human DNA, and that contamination with modern DNA is less of a problem, has led in some cases to a laxity in research standards with insufficient attention paid to the need to authenticate ancient DNA results. We illustrate our concerns by reference to a recent paper reporting biomolecular detection of ancient TB DNA in skeletons from the eastern Mediterranean Neolithic settlement of Atlit-Yam. We are unconvinced that the skeletal evidence presented in this paper gives sufficient indication of TB to warrant destructive analysis, and we are concerned that during the biomolecular part of the project inadequate attention was paid to the possibility that results might be due to laboratory cross-contamination or to amplification of environmental mycobacterial DNA present in the bones.     

CLASS OR COMMUNITY? MARX,THE RUSSIAN COMMUNE,AND CONTEMPORARY CRITICAL THEORY

This article explores the relationship between class and community through a discussion of peasant struggles and the commune during the Russian Revolution. Doing so, we show how Marx's class‐oriented reflections on community can help us to understand the role that the peasantry plays (or should play) in processes of social transformation. This enables us, first, to understand the relevance of communal forms for Marx, who believed that communitarian ways of life were crucial for overcoming a value‐based society. It is, in fact, a mistake to divide Marx's intellectual trajectory into two periods: a categorical Marx, who authored Capital and critically analyzed the classical theory of value, and a phenomenological, empirical Marx, who in the last years of his life abandoned writing Capital and focused instead on studying the Russian peasantry. Second, it enables us to discuss new externalist visions, such as postcolonial and decolonial theories, which postulate that the subordination of contemporary peasant communities is rooted in epistemology, culture, and local power relations. These theories are related to the old social‐democratic canon, which conceives of social classes as preconstituted entities and of capital as a parasitic externality that is incommensurable with social dynamics. The experience of the Russian peasantry calls into question all externalist and ontological perspectives.     

Mitochondrial DNA haplotypes of Devensian hyaenas from Creswell Crags,England

Spotted hyaena (Crocuta crocuta) remains have been recovered from British Middle and Upper Pleistocene sites at intervals within the period 700–730 ka BP. Morphological studies have suggested that hyaenas of the Last Interglacial sensu stricto (Ipswichian: Marine Isotope Stage [MIS] 5e, 130–115 ka BP) and Last Glacial (Devensian: MIS 3, 61–24 ka BP) were two distinct populations, the Ipswichian hyaenas becoming extinct in Britain during MIS 5 and the Devensian ones arriving via a subsequent migration from continental Europe. However, the apparent presence of hyaenas in later MIS 5 deposits has led to the alternative suggestion that there was a southern relict population from which the Devensian hyaenas originated. We obtained ancient DNA (aDNA) sequences from four Devensian hyaena specimens from Creswell Crags, Derbyshire, dated to around 45 ka ¹⁴C BP. Each of these four specimens belonged to the mitochondrial DNA (mtDNA) clade A. This clade is not thought to have been present in Europe until ~360 ka BP, after the initial arrival of hyaenas in Britain. The DNA results, therefore, suggest that there were at least two waves of hyaena dispersals into Britain. The results are consistent with the repeated dispersals into Britain of another Pleistocene social carnivore, Homo sapiens.     

THE “THEORETICAL REVOLUTION” IN INTELLECTUAL HISTORY: FROM THE HISTORY OF POLITICAL IDEAS TO THE HISTORY OF POLITICAL LANGUAGES

This article intends to clarify what distinguishes the so‐called new “politico‐intellectual history” from the old “history of political ideas.” What differentiates the two has not been fully perceived even by some of the authors who initiated this transformation. One fundamental reason for this is that the transformation has not been a consistent process deriving from one single source, but is rather the result of converging developments emanating from three different sources (the Cambridge School, the German school of conceptual history or Begriffsgeschichte, and French politico‐conceptual history). This article proposes that the development of a new theoretical horizon that effectively leads us beyond the frameworks of the old history of political ideas demands that we overcome the insularity of these traditions and combine their respective contributions. The result of this combination is an approach to politico‐intellectual history that is not completely coincident with any of the three schools. What I will call a history of political languages entails a specific perspective on the temporality of discourses; this involves a view of why the meaning of concepts changes over time, and is the source of the contingency that stains political languages.