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In this article, we explore how normative ideologies of good mothering are being reproduced and contested through urban homesteading, a sustainable lifestyle that emphasizes household self-provisioning. Urban homesteading practices may include gardening and urban agriculture, canning, and pickling, and a variety of do-it-yourself and craft projects. Based on qualitative research with 19 urban homesteading households with children in the Boston and Chicago Metropolitan areas, we argue that urban homesteading discourses and practices reflect and align with intensive mothering ideologies in the United States. Intensive mothering ideologies encourage a selfless devotion of physical, emotional, and mental energy to childrearing, and are often associated with individualized, privileged, and gendered subjectivities. We find these intensive mothering ideologies especially visible in the ways that mothers perceive and respond to environmental risk by adopting and enacting urban homesteading labors. We also note that the choice to respond to risk by homesteading is often, but not always, mediated and animated by economic, temporal, and social privilege. In this way, urban homesteading and surrounding discourses may inadvertently raise the bar of ‘good’ motherhood in ways that demand more of women and marginalize or burden mothers with less resources and privilege. However, rather than dismiss homesteading entirely on these grounds, we suggest that it may be possible to harvest impulses of care, connection, and collectivity associated with homesteading in ways that benefit rather than burden all mothers.  相似文献   
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The Internet is growing in popularity as a research site and is often framed as the next frontier in human subjects research. The opportunities the Internet provides for political organizing, making personal experiences more public, and creating spaces for a variety of voices makes it particularly relevant to feminist geographers and researchers such as ourselves. However, many qualitative researchers approach online research as though the Internet simply archives an abundance of data that is ‘there for the taking.’ Being trained in feminist research methods, we took issue with this approach, yet also encountered challenges when trying to apply feminist practices and ethical perspectives to online research environments. We explore these challenges through a collaborative reflection on our own independent online research experiences. Three themes emerge: (1) interpreting politics and visibility in online spaces, (2) researcher positionality across virtual and material study sites, and (3) subjectivity and power in online research ethics. Reflecting on these themes, we argue that the insights of feminist ethics and a feminist geographical lens are crucial for bringing much-needed reflexivity and reciprocity into online research. Simultaneously, online research opens up exciting new ways of conceptualizing central ideas within feminist research ethics, including politicization, positionality, and power.  相似文献   
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High molecular weight long-chain mycolic acids are key structural components of the cell envelope of Mycobacterium tuberculosis and they are established as biomarkers for the identification of both ancient and modern tuberculosis. Mycolic acids from M. tuberculosis have a characteristic profile, reflecting contributions from five major distinct homologous series of mycolate structural types. Diagnosis of tuberculosis in archaeological material, using mycolic acid biomarkers, depends on objective recognition of the key characteristic mycolic acid components. A recent article in this journal claimed that tuberculosis could be confirmed in ancient bones by high throughput mass spectrometric analysis of mycolic acids. Scrutiny of the data presented reveals no convincing evidence for the presence of mycolic acids, characteristic of the M. tuberculosis complex, in the skeletal remains examined. This communication reviews the essential criteria necessary for positive tuberculosis diagnosis, using mycolic acids.  相似文献   
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This paper investigates the impact of the main criterion employed by the European Commission for the allocation of the largest portion of Structural Funds, based on the threshold of the 75% of European Union (EU) average gross domestic product (GDP) per capita. We focus on the 2014–2020 programming period and on EU-15 regions to analyze if this criterion has penalized some of them, as a consequence of the 2004 EU enlargement, which has represented an exogenous shock in the allocation process, due to the economic backwardness of new member states. Through the application of Synthetic Control Methods and Difference-in-Differences estimators at different geographical scales, we show that regions that did not obtain the less developed status in both the programming period 2007–2013 and 2014–2020, but that would have obtained it in the period 2014–2020 without the 2004 EU enlargement, experienced a significantly lower GDP per capita growth between −10.5% and −5.7%. Conversely, territories that in the period 2014–2020 lost the less developed status, previously obtained in the time frame 2007–2013, were not characterized by a significantly lower economic growth, providing some evidence of the effectiveness of the safety net.  相似文献   
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We have amplified Mycobacterium leprae DNA from the skeleton of an adult human female exhibiting signs of lepromatous leprosy (LL). The remains were excavated from the site of Devkesken 6 on the Ustyurt plateau of Uzbekistan and date to between the 1st and 4th centuries AD. Recovered DNA was fragmented but of sufficient quality and quantity to allow a series of biomolecular genotyping methods to be applied. These methods included variable nucleotide tandem repeat (VNTR) typing of two microsatellite and one minisatellite regions and also single-nucleotide polymorphism (SNP) typing for nine informative loci.Genotyping showed that the causative strain of M. leprae exhibited a SNP-type 3 profile, characteristic of cases associated geographically with Europe and North Africa. Further SNP sub-typing was performed and the data obtained from the Uzbek leper was compared with the same loci amplified from a case of LL recovered from Blackfriars, Ipswich, UK dating to between the 13th and 16th centuries AD. Unique group 3 subtypes were found in both the Uzbek case and Ipswich 1914. These appear to be ancestral to recent type 3 strains. Mycolic acid analysis confirmed the presence of M. leprae in the Uzbek samples. Phylogenetically informative SNPs and other polymorphic loci will contribute to the study of human migrations, as well as the origin and spread of leprosy.  相似文献   
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It is sixteen years since the first detection of Mycobacterium tuberculosis DNA in archaeological specimens, yet the validity of findings continues to be questioned. Rigorous scientific scrutiny and debate is valuable and has led to a coalescence of procedures and precautions amongst those actively engaged in this work. It is disappointing that these good practices are not recognised by certain scientists whose primary expertise is in the related fields of archaeology, palaeopathology, and eukaryote ancient DNA. There is a danger that by constant repetition, disputable and inadequately justified concerns will assume the status of self-perpetuating myths and misunderstandings. We discuss these issues with reference to a recent article in this journal, in which clear peer-reviewed scientific data were specifically targeted as part of a general critique of the field of the palaeomicrobiology of tuberculosis. We believe we have given sufficient evidence and cogent argument to persuade the unbiased reader that the views in the critique by Wilbur et al. are unjustified.  相似文献   
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We classify sites based on their predominant period computed using average horizontal-to-vertical (H/V) response spectral ratios and examine the impact of this classification scheme on empirical ground-motion models. One advantage of this classification is that deep geological profiles and high shear-wave velocities are mapped to the resonance frequency of the site. We apply this classification scheme to the database of Fukushima et al. [2003] Fukushima, Y., Berge-Thierry, C., Volant, P., Griot-Pommera, D. A. and Cotton, F. 2003. Attenuation relation for west Eurasia determined with recent near-fault records from California, Japan and Turkey. Journal of Earthquake Engineering, 7(3): 126.  [Google Scholar], for which stations were originally classified as simply rock or soil. The calculation of average H/V response spectral ratios permits the majority of sites in the database to be unambiguously classified. Soft soil conditions are clearly apparent using this technique. Ground-motion prediction equations are then computed using this alternative classification scheme. The aleatoric variability of these equations (measured by their standard deviations) is slightly lower than those derived using only soil and rock classes. However, perhaps more importantly, predicted response spectra are radically different to those predicted using the soil/rock classification. In addition, since the H/V response spectral ratios were used to classify stations the predicted spectra for different sites show clear separation. Thus, site classification using the predominant period appears to be partially mapped into the site coefficients of the ground-motion model.  相似文献   
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