Analysis of ancient Japanese society through mitochondrial DNA sequencing

In this study, DNA was extracted from human bones recovered from a 2000-year-old archaeological site located in northern Kyushu in southwestern Japan. Part of the mitochondrial control region was amplified by a polymerase chain reaction. Mitochondrial DNA sequences determined from 55 individuals were classified into 16 different types. Comparing the location of the burial site and the sequence types, people buried at separate sites were shown to have different maternal lineages. Our palaeomolecular biological findings strengthen the opinion that social differentiation began during this period in Japan, a fact that is generally accepted among archaeologists. The results of this study show that intensive analysis of ancient DNA from archaeological sites is a useful tool for investigating the social systems of vanished populations.