The survival of PCR-amplifiable DNA in cow leather

We have investigated the survival of PCR-amplifiable mitochondrial and nuclear DNA in a small number of modern and medieval bovine leather samples. The results of this preliminary investigation demonstrate that, while no nuclear DNA can be PCR-amplified from any of the specimens, mitochondrial DNA can be amplified from all samples. To investigate this contrasting pattern of DNA survival further, we have vegetable-tanned cow skin in our own laboratory, and directly assayed the survival of PCR-amplifiable mitochondrial and nuclear DNA at each step of the process. The results indicate that nuclear DNA is reduced to sub-amplifiable levels as a result of the tanning baths, whereas amplifiable mitochondrial DNA survives the complete process. Our results suggest that old and archaeological bovine leather may represent a useful source of genetic information, although this information will most likely be limited to that which can be gained from mitochondrial DNA.     

Insurgents against Brussels: Euroscepticism and the right-wing populist turn of the Lega Nord since 2013

Under the leadership of Matteo Salvini, the Lega Nord has shifted away from its previous political identity as a voice for Italy’s north and has placed hostility towards the policies and institutions of the European Union (EU) at the heart of its rhetoric. Nowadays, the enemy is Rome no longer: it is Brussels, European institutions, and the threat to the national sovereignty posed by the EU. Borrowing from the Italian political philosopher Nicola Matteucci, we would describe Salvini’s Lega as a ‘populist insurgency’. That is to say, it is a populist party that marries the traditional populist evocation of the virtues of the people against the corrupt elites, with a pervasive glibness of analysis.     

Pleistocene frogs from the Darling Downs,southeastern Queensland,and their palaeoenvironmental significance

Systematic collecting from fluvial late Pleistocene deposits from the Darling Downs, southeast Queensland, Australia, has led to the recovery of the first fossil frogs from the region, ail from the Myobatrachidae, a family of ground dwelling and burrowing frogs. The most common species recovered, Limnodynastes tasmaniensis, is extant on the Darling Downs. The fossil taxa include species whose extant populations inhabit arid zones(Limnodynastes sp. cf. L. spenceri), montane forests (Kyarranus spp.), and open woodlands (Neobatrachus sudelli), and indicate the existence of a mosaic of habitats during the Pleistocene. The absence of the Hylidae (tree frogs), a family common throughout the Darling Downs today, may be explained by a taphonomic bias that favours non-arboreal forms. Alternatively, hylids may have been rare or absent on the Darling Downs during the Pleistocene.     

A Covering Tour Model for Planning Mobile Health Care Facilities in SuhumDistrict, Ghama

The provision of adequate primary health care in developing countries is often troublesome. The problem is to provide a sufficient number of facilities to be geographically accessible, yet few enough to be properly stocked and staffed. In many less developed countries accessibility problems are exacerbated by extensive rainy seasons in which travel is only possible on paved roads. Using the covering tour model we investigate the use of mobile facilities to resolve this dilemma in Suhum District, Ghana. The model minimizes a mobile facility's travel while serving all population centers within range of a feasible stop. Computational results show that in the rainy season the model cannot provide full coverage; over six percent of the population is beyond a covering distance of eight kilometers. In the dry season, 99 percent of the population can be served by a tour at a covering distance of seven kilometers.Beyond a distance of four kilometers, the dry season problem becomes a trade-off between the distance traveled by healthcare patrons and mobile facilities. These results illustrate the importance of flexibility of mobile systems: if accessibility cannot be provided in all seasons it may still be provided at favorable times of the year.