Effects of Sodium Hydroxide Treatment and Ultrafiltration on the Removal of Humic Contaminants from Archaeological Bone

This study compared bone collagen extraction techniques that included treatment with sodium hydroxide and 30 kDa ultrafilters using a set of well‐preserved, humic‐contaminated archaeological marine mammal bones. Treatment with sodium hydroxide was effective at removing humic contaminants from archaeological bone, although yields were significantly decreased. Yields were also significantly decreased by ultrafiltration although this study produced no evidence that 30 kDa ultrafilters were effective at selectively removing humic contaminants from archaeological bone. The combination of sodium hydroxide treatment and ultrafiltration did not produce superior results to the treatment involving only sodium hydroxide. Archaeological samples exhibiting darker colouration indicative of humic contamination should be treated with sodium hydroxide to remove these contaminants. Copyright © 2017 John Wiley & Sons, Ltd.     

Isotopic Evidence for Marine Consumption and Mobility in the Atacama Desert (Quillagua,Northern Chile)

Archaeological research in the Atacama Desert has recovered evidence of considerable cultural variability. This variability seems to have increased during the Late Intermediate Period (AD 950–1400). The oasis of Quillagua, located at the margins of the Loa River in northern Chile, between the Andes and the coast (70 km from the Pacific Ocean), has shown important evidence regarding this cultural diversity. The variety in the archaeological evidence found at Quillagua has been interpreted as the result of two different cultural influences at the oasis: the Pica‐Tarapacá who occupied the coastal and inland regions to the north of Quillagua and the Atacama who occupied the oasis and fertile areas southeast of Quillagua. Here, we present the results of stable carbon, nitrogen and oxygen isotope analyses of 23 individuals recovered from the Cementerio Oriente in Quillagua, in order to test whether the observed cultural variability is also reflected in diet and mobility patterns. Results from carbon and nitrogen isotope measurements indicate the importance of marine protein, as well as a contribution of maize in the diet of some individuals. Four individuals show low δ¹⁸O values, suggesting a possible highland or non‐local origin, whereas values for the remaining individuals are consistent with lowland populations. Together, the results support the idea that the Quillagua oasis represented an important site of interaction between the Tarapacá and Atacama cultures, with close contacts with the coast but also with the presence of individuals from the highlands. Copyright © 2015 John Wiley & Sons, Ltd.     

An Osteobiography of a 19th‐Century Dog from Toronto,Canada

A 19^th‐century dog burial uncovered from a historical homelot in Toronto, Canada, provided a unique opportunity to reconstruct the individual's osteobiography. Of particular interest are the dog's very large size and a suite of skeletal pathologies. Recovery of a nearly complete skeleton combined with the use of X‐rays and micro‐computed tomography (micro‐CT) allowed for a discriminating differential diagnoses. Stable isotope analyses were applied to investigate questions of diet. Results reveal an individual who suffered greatly from disease towards the end of his life and hint at its owners attitudes towards dogs. The interdisciplinary approach applied to this case study highlights the potential information obtainable from pet burials. We argue that better analyses and reporting of pet burials will help address research questions targeting broader themes related to human–animal relationships. Copyright © 2015 John Wiley & Sons, Ltd.     

Exponentially decreasing tooth growth rate in horse teeth: implications for isotopic analyses

A range of archaeological and palaeoclimatic studies use isotopic analyses of ungulate hypsodont tooth enamel. Such studies commonly assume a constant growth rate, though this has not been fully tested. Here, we use stable isotope analyses of sequential enamel samples to study horse tooth growth. We fit the data using models corresponding to constant and exponentially decreasing rates of growth, and compare the results to metrical data showing the geometry and timing of apposition. The results indicate enamel apposition and maturation advances at an exponentially decreasing rate. An understanding of this variable growth rate is crucial for interpreting isotopic data from equid teeth.     

Stories behind the fortress: Stable isotope analysis and 14C dating of soldiers' remains from the Bayanbulag site,Mongolia

Stable carbon and nitrogen isotope analysis of 15 individuals buried closed to Bayanbulag site or the Shouxiangcheng fortress in southern Mongolia revealed complex dietary features. Only one individual had a diet similar to that of the pastoral population in northern China, while the others, who had varying carbon isotope values and low nitrogen values, might have been from different agricultural areas. ¹⁴C dating results suggest that the earliest interment could be much earlier than the building of Shouxiangcheng fortress recorded in Han texts. Analysis of the burial arrangement further confirmed that these deceased were not buried at the same time.     

Cleft Lip Case in a Middle Bronze Age Young Man from Altai,Russia

Oral cleft (OC) is a common congenital anomaly in humans, which occurs in approximately one per 700 live births. However, cleft skulls are found relatively seldom in archaeological specimens, especially for the bc period. Mortality of infants with OC was apparently high because of breastfeeding difficulties and culturally sanctioned rejection of them in the past. This paper presents one of the most ancient cases of OC in Eurasia. The finding comes from the Middle Bronze Age pastoralists' cemetery in Altai, Russia. The skeletal remains have been radiocarbon dated to 1883–1665 cal bc . Cranial and dental measurements, computed tomography and radiography have been used to examine this malformation. The facial sculpting reconstruction has been performed to visualise the ante mortem appearance of the cleft face. Besides, δ¹³C and δ¹⁵N isotope analysis was used for a comparative study of the diet of the cleft individual. It has been established that a young man from Altai was affected by complete bilateral cleft lip and alveolus, which is a very rare form of typical OC. This defect is associated with dental anomalies such as hypodontia, microdontia, and ectopia as well as with dysmorphology of the facial complex outside the cleft area, including mild hypertelorism, hypoplasia of nasal bones and antheroposterior maxillary deficiency. Furthermore, aplasia of the frontal sinuses and the sacral spina bifida occulta have been observed. These findings are consistent with clinical data indicating strong correlation between development of a major defect and multiple minor anomalies. The results of the study also suggest that there was tolerant attitude towards the facial deformities among the ancient Altai pastoralists. Copyright © 2016 John Wiley & Sons, Ltd.     

The Zoutsteeg Three: Three New Cases of African Types of Dental Modification from Saint Martin,Dutch Caribbean

Dental modification was widely practiced in sub‐Saharan Africa as a form of cultural expression, and during the era of the transatlantic slave trade, it was regularly identified in enslaved Africans who were transported to the Americas. Here, we report three new cases of African types of dental modification from the Caribbean island of Saint Martin that were recently encountered during construction activities in the Zoutsteeg area of Philipsburg, the capital of the Dutch half of the island. The artifacts associated with the burials indicate that they date to the late 17^th century, prior to the foundation of the town of Philipsburg in 1735. The dental evidence further suggests that the three individuals were born in Africa, as opposed to the Americas. This could be confirmed by tooth enamel strontium isotope measurements which yielded values that are inconsistent with an origin in the Caribbean but consistent with an origin in Africa. Unfortunately, neither the dental patterns nor the strontium isotope values allow us to determine their specific origins in Africa. However, both the methods used to modify the teeth and the isotope ratios suggest that the ‘Zoutsteeg Three’ originated in different parts of Africa. Copyright © 2012 John Wiley & Sons, Ltd.     

Monitoring fluid chemistry in iron oxide–copper–gold‐related metasomatic processes,eastern Mt Isa Block,Australia

Most researchers in the Proterozoic eastern Mt Isa Block, NW Queensland, Australia, favour magmatic fluid and salt sources for sodic‐(calcic) alteration and iron oxide–copper–gold mineralization. Here we compare spatial, mineralogic and stable isotope data from regional alteration assemblages with magmatic and magmatic‐hydrothermal interface rocks in order to track chemical and isotopic variations in fluid composition away from inferred fluid sources. Tightly clustered δ¹⁸O values for magnetite, quartz, feldspar and actinolite for igneous‐hosted samples reflect high temperature equilibration in the magmatic‐hydrothermal environment. In contrast, these minerals record predominantly higher δ¹⁸O values in regional alteration and Cu–Au mineralization. This dichotomy reflects partial equilibration with isotopically heavier wallrocks and slightly lower temperatures. Increases in Si concentrations of metasomatic amphiboles relative to igneous amphiboles in part reflect cooling of metasomatic fluids away from igneous rocks. Variations in X_Mg for metasomatic amphiboles indicate local wallrock controls on amphibole chemistry, while variations in X_Cl/X_OH ratios for amphiboles (at constant X_Mg) indicate variable aH₂O/aHCl ratios for metasomatic fluids. Biotite geochemistry also reflects cooling and both increases and decreases in aH₂O/aHCl for fluids away from plutonic rocks. Decreased aH₂O/aHCl ratios for metasomatic fluids reflect in part scavenging of chlorine out of meta‐evaporite sequences, although this process requires already saline fluids. Local increases in aH₂O/aHCl ratios, as well as local decreases in δ¹⁸O values for some minerals (most notably haematite and epithermal‐textured quartz), may indicate ingress of low salinity, low δ¹⁸O fluids of possible meteoric origin late in the hydrothermal history of the region. Taken together, our observations are most consistent with predominantly magmatic sources for metasomatic fluids in the eastern Mt Isa Block, but record chemical and isotopic variations along fluid flow paths that may be important in explaining some of the diversity in alteration and mineralization styles in the district.     

Fracture sealing and fluid overpressures in limestones of the Jabal Akhdar dome,Oman mountains

Fractures are important conduits for fluid flow in the Earth's crust. To better understand the spatial and temporal relations among fracturing, fracture sealing, and fluid flow, we have studied fractures, faults, and veins in a large dome (Jabal Akhdar) in the Oman mountains. Our work combines the results of meso‐ and microstructural analyses and stable isotope analyses. Seven generations of fractures and veins have been identified in the carbonate‐dominated dome. The earliest generations of veins developed during extension and subsidence of the Mesozoic basin. These veins formed in the inclined segments of bedding‐parallel stylolites and in extensional fractures that are perpendicular to bedding (#1 and #2, respectively). These extension‐related veins are truncated by bedding‐parallel veins (#4) that formed during top‐to‐north bedding‐parallel shear of both the northern and southern limbs of the dome. These veins are consistent with a change in stress regime and may be related to an earlier generation of strongly deformed pinch‐and‐swell veins (#3) that are exposed locally on the southern limb of the dome. Normal faults contain a set of en‐echelon tension gashes (#5) and veins emplaced in dilational jogs along the fault planes (#6). In the northern part of the dome, veins (#7) associated with thrusts post‐date the normal faults. Samples of veins and their host rocks were analyzed to provide information on fluid‐rock interaction in the dome and the scale(s) of fluid movement. Oxygen isotope values range from +16.2 to +29.3‰; carbon isotope values range from 0 to +3.6‰. The results of the structural and isotopic analyses are consistent with the early veins (#2–#5) having precipitated from overpressured fluid in a isotopically rock‐buffered system. During normal faulting (#5 and #6), a more open system allowed external fluid to infiltrate the dome at drained conditions and precipitate the youngest sets of veins (#6 and #7).     

Trends in pig product processing at British Neolithic Grooved Ware sites traced through organic residues in potsherds

Gas chromatography (GC), GC-mass spectrometry (GC-MS) and GC-combustion-isotope ratio MS (GC-C-IRMS) analyses of absorbed and surface lipid residues preserved in potsherds were used to explore the extent of pig product processing exploitation in the later British Neolithic Grooved Ware tradition. Assessments were made regarding whether porcine lipids were associated with specific Grooved Ware traits, i.e. decoration, substyle, geographical area and type of site. Two hundred and twenty-two Grooved Ware potsherds were analysed, 70% of which contained lipid concentrations considered significant (>5 μg g⁻¹). All the lipid residues were dominated by animal fats, although plant and beeswax were also detected in a small number of extracts. δ¹³C values of the major fatty acid components of degraded animal fats (C_16:0 and C_18:0) were determined for 126 extracts and used to assign ruminant or porcine origins to the residues; 16% of these were found to have a predominantly porcine isotope signature. Statistical associations with pig exploitation were shown to exist with substyle, geographical area and site type, whereas, no relationship was seen between decoration and the type of commodity processed. Intact triacylglycerols were preserved in 19% of the sherds; half of these had distributions consistent with the identifications based on δ¹³C values, the remainder differed either due to the presence of mixed commodities or because lower molecular weight homologues had been lost due to degradation. In addition to the detection of pig exploitation, results from lipid residue analysis showed a good correlation with faunal assemblages, suggesting that stable isotope analysis may be used as a proxy for animal exploitation at sites where bones have not survived.