Stable Isotopic Analysis of Human Skeletons from the Sunhung Mural Tomb,Yeongju, Korea: Implication for Human Diet in the Three Kingdoms Period

This study reports the results of stable isotope analyses (carbon, nitrogen and sulphur) of human bone collagen from the Sunhung mural tomb from the Three Kingdoms Period of Korea (AD 300–668). The stable isotope data indicate that the main source of protein in the diet of the interred seven Sunhung individuals came from C₃‐based terrestrial resources, and there was an isotopic variation between individuals at this site. To investigate dietary patterns in the Three Kingdoms period, we compared our results with reported isotopic data from other southeastern Three Kingdoms sites (Imdang‐dong, Songhyeon‐dong and Yean‐ri). We found that the Sunhung individuals had similar isotope ratios to the people from these other sites. However, there was noticeable isotopic difference among individuals from each study site, although much of the dietary protein in each site was mainly from terrestrial sources. We propose that the most parsimonious explanation for this isotopic pattern is variation in social status during this time period. Copyright © 2012 John Wiley & Sons, Ltd.     

New Insights into Mesolithic Human Diet in the Mediterranean from Stable Isotope Analysis: The Sites of Campu Stefanu and Torre d'Aquila,Corsica

Mesolithic human remains are rare in the archaeological record of the French Mediterranean. Only the island of Corsica has so far produced relatively well‐preserved burials, and recent archaeological excavations have brought to light new Mesolithic human remains. The site of Campu Stefanu , located in Sollacaro in the southeast of the island, contained a collective burial of seven to eight individuals in a previously unobserved funerary context. A re‐evaluation of collections in regional museums yielded the remains from another Mesolithic individual from the site of Torre d 'Aquila , excavated at Pietracorbara, in the northern part of the island, at the beginning of the 1990s. These two discoveries presented the rare opportunity to obtain new radiocarbon dates and paleodietary insights from this crucial time period using stable isotope analysis (δ¹³C, δ¹⁵N) on collagen. From Campu Stefanu, one individual had sufficient collagen preserved for radiocarbon dating, revealing that it is the oldest Mesolithic human known on the island, dated to 10216–9920 cal. BP. At Torre d'Aquila, radiocarbon dates indicate that the individual belonged to a younger Mesolithic phase than Campu Stefanu, dated to 9903–9596 cal. BP. δ¹³C and δ¹⁵N isotope ratios are similar between the Campu Stefanu and Torre d'Aquila individuals and indicate a diet dominated by the consumption of terrestrial animal protein and a lack of marine resources. These findings are in contrast with the previous results from two other Mesolithic individuals from Corsica from the sites of Araguina Sennola and Monte Leone , for which about 25–30% of the consumed proteins came from a marine diet. The dietary variability recorded in Corsica is consistent with results obtained from Mesolithic human remains of Sicily and the Iberian Peninsula. We can hypothesise, that despite the nomadic lifestyle, the distance to the sea played a major role in Mesolithic food choices in Corsica. Copyright © 2016 John Wiley & Sons, Ltd.     

A Palaeodietary Study of Stable Isotope Analysis from a High‐status Burial in the Copper Age: The Montelirio Megalithic Structure at Valencina de la Concepción–Castilleja de Guzmán,Spain

This palaeodietary study presents carbon and nitrogen stable isotope data from human and faunal skeletal remains from the Copper Age settlement of Valencina de la Concepción–Castilleja de Guzmán, located in Seville, Spain. Montelirio, the only Valencina–Castilleja human group from which we have obtained reliable palaeodietary results, had a diet based on C₃ terrestrial resources, including both plants and animals. The protein component of the diet consisted mainly of meat, milk and dairy products from livestock as well as C₃ plant protein from cereals and pulses. This study compares data from Montelirio, the Copper Age group from Valencina–Castilleja, with the published data from other Iberian Late Neolithic–Copper Age (LN‐CA) societies. This comparison reveals a homogeneous diet with some exceptions. Overall, the LN‐CA diet in the Iberian Peninsula consisted mainly of animal proteins from meat, milk and dairy products from livestock as well as C₃ plant sources from the characteristic agriculture of the societies of these periods. This study also demonstrates the minor use of aquatic resources from the Neolithic to the Copper Age period in the Iberian Peninsula. Copyright © 2015 John Wiley & Sons, Ltd.     

Investigating Dietary Patterns with Stable Isotope Ratios of Collagen and Starch Grain Analysis of Dental Calculus at the Iron Age Cemetery Site of Heigouliang,Xinjiang, China

Here, we present δ¹³C and δ¹⁵N results for the dietary reconstruction of nomadic pastoralists from the Iron Age (ca. 1000 bc –8 ad ) site of Heigouliang. The human (n = 27) δ¹³C values range from −19.6‰ to −17.0‰ with a mean value of −18.5 ± 0.5‰, and the δ¹⁵N results range from 11.5‰ to 13.8‰ with a mean value of 12.4 ± 0.6‰. The results indicated that animals, like sheep, were part of the predominately C₃ terrestrial diet, but two individuals have values greater than −18‰ that is indicative of some input of C₄ foods in their diets. Because of a lack of faunal samples and to supply complementary information concerning plant consumption, teeth from four individuals were analysed for dental calculus microfossils. Starch grains were found to correspond to Triticeae and Poaceae, possibly including wheat (Triticum aestivum), barley (Hordeum vulgare), highland barley (H. vulgare L var. nudum), foxtail millet (Setaria italica) and/or common millet (Panicum miliaceum). At the population level, no dietary differences were detected between burial owners and sacrificial victims, but variations were found when specific tombs were analysed. In particular, individuals with bone trauma associated with armed conflict also had distinct isotopic signatures possibly suggesting that some of the sacrificial victims could have been captured warriors that were sacrificed for the burial owners. While limited, the results are some of the first from an Iron Age population from Xinjiang and contribute to our understanding of the dietary patterns of this region. Copyright © 2015 John Wiley & Sons, Ltd.     

Prehistoric dietary adaptations among hunter-fisher-gatherers from the Little Sea of Lake Baikal,Siberia, Russian Federation

Dietary adaptations of prehistoric hunter-gatherers from Neolithic and Early Bronze Age cemeteries in the Little Sea region of Cis-Baikal (the region to the west and north of Lake Baikal) are explored using stable isotopes of carbon and nitrogen. Stable isotope data, including stable carbon isotopes from bone carbonate, are presented for 22 individuals from the site of Kurma XI, dated to approximately 6500 B.P. to 4000 B.P. Data are compared to previously analyzed individuals from the larger Early Bronze Age cemetery, Khuzhir-Nuge XIV (Katzenberg et al., 2009 JAS) and to smaller sites located along the shore of the Little Sea, including sites on Olkhon Island. An extensive collection of fauna, both prehistoric and modern, from the Little Sea and neighboring regions is also analyzed for stable isotopes of carbon and nitrogen. Clear distinctions are found in modern fish recovered from the Little Sea, in contrast to those from the open waters of the lake and from the neighboring Angara and Lena rivers. Considerable variation is seen in stable carbon isotope ratios from fish while stable nitrogen isotope ratios are not as variable, regardless of habitat. Isotope source modeling is used to assist in reconstructing past dietary adaptations. While there is ample evidence from other studies for cultural change over this temporal span, diet appears to have been relatively stable.     

Stable nitrogen isotope values in arid‐land kangaroos correlated with mean annual rainfall: potential as a palaeoclimatic indicator

Bone collagen stable nitrogen isotope values are reported for modern kangaroos (Macropus spp.) from eight field sites along a 1160 km south–north transect from temperate coastal to arid interior South Australia, in order to investigate δ¹⁵N tissue variability in relation to rainfall and relative humidity. Mean annual rainfall along the transect ranges from 775 mm at Mount Gambier in the southeastern coast to 176 mm at Innamincka in the arid northern interior, while 3pm relative humidity ranges from 73% at coastal Flinders Chase to 36% at Innamincka. In arid habitats (176–238 mm rainfall), the δ¹⁵N values of kangaroo bone collagen become more positive in relation to decreasing mean annual rainfall (r² = 0.98), while there is only a weak correlation with relative humidity (r² = 0.67). In contrast, in temperate and semi‐arid coastal habitats (350–775 mm), there is no correlation between kangaroo bone collagen δ¹⁵N values and rainfall (r² = 0.0011) or between δ¹⁵N values and relative humidity (r² = 0.0035). Thus, in South Australia, kangaroo bone collagen δ¹⁵N values do not show a simple linear correlation with either rainfall or relative humidity across all habitats. These results suggest that stable nitrogen isotope analyses of herbivore bones obtained from archaeological and palaeontological sites may provide a reliable proxy for past rainfall values in arid‐land ecosystems. Copyright © 2007 John Wiley & Sons, Ltd.     

Gas breakthrough experiments on pelitic rocks: comparative study with N2, CO2 and CH4

The capillary‐sealing efficiency of intermediate‐ to low‐permeable sedimentary rocks has been investigated by N₂, CO₂ and CH₄ breakthrough experiments on initially fully water‐saturated rocks of different lithological compositions. Differential gas pressures up to 20 MPa were imposed across samples of 10–20 mm thickness, and the decline of the differential pressures was monitored over time. Absolute (single‐phase) permeability coefficients (k_abs), determined by steady‐state fluid flow tests, ranged between 10^?22 and 10^?15 m². Maximum effective permeabilities to the gas phase k_eff(max), measured after gas breakthrough at maximum gas saturation, extended from 10^?26 to 10^?18 m². Because of re‐imbibition of water into the interconnected gas‐conducting pore system, the effective permeability to the gas phase decreases with decreasing differential (capillary) pressure. At the end of the breakthrough experiments, a residual pressure difference persists, indicating the shut‐off of the gas‐conducting pore system. These pressures, referred to as the ‘minimum capillary displacement pressures’ (P_d), ranged from 0.1 up to 6.7 MPa. Correlations were established between (i) absolute and effective permeability coefficients and (ii) effective or absolute permeability and capillary displacement pressure. Results indicate systematic differences in gas breakthrough behaviour of N₂, CO₂ and CH₄, reflecting differences in wettability and interfacial tension. Additionally, a simple dynamic model for gas leakage through a capillary seal is presented, taking into account the variation of effective permeability as a function of buoyancy pressure exerted by a gas column underneath the seal.     

Stable carbon and nitrogen isotope analysis on human remains from the Early Mesolithic site of La Vergne (Charente-Maritime,France)

We report here the results of stable carbon and nitrogen isotope analysis of human and faunal remains from La Vergne (Charente-Maritime, western France), a rare Early Mesolithic burial site (ca. 8500–8000 cal BC). The results for nine humans (average δ¹³C = −19.3‰; δ¹⁵N = 9.4‰) indicate a strongly terrestrial diet, dominated by animal protein, with the possibility of, at best, a slight contribution of marine-derived protein. Given lower sea-levels in the early Holocene, the site would have been some 60–80 km from the sea at the time of its use; nevertheless, contacts with the coast are shown by the presence of numerous marine shell beads in the graves. In the light of the stable isotope results, it is suggested here that such contacts most likely took the form of exchange with coastal communities whose remains now lie underwater.     

Paleodietary analysis of the prehistoric population of the Canary Islands inferred from stable isotopes (carbon,nitrogen and hydrogen) in bone collagen

Nitrogen and carbon isotope compositions were measured in the bone collagen from a total of 86 prehispanic samples of the Canary Islands, and hydrogen in 70, all of them with enough amount of bone collagen, and adequate N and C content. These samples belong to prehistoric population of El Hierro (n = 27), Tenerife (n = 18), and Gran Canaria (n = 41). Isotope compositions were also obtained for prehistoric and modern food resources that were likely consumed by these people. Marked differences were observed among the three islands regarding the three isotopes analyzed: the δ¹⁵N values were highest among the population of Gran Canaria (10.8‰ ± 0.9‰), who also showed the highest δD values (7 ± 8‰). The population of El Hierro showed the highest δ¹³C values (−18.6‰ ± 0.7‰). These data suggest a high consumption of marine products by the population from El Hierro, and also an important consumption of terrestrial meat or marine, piscivore fish, by the population from Gran Canaria, together with domesticated C₃ plants (barley and/or wheat), fruits of Ficus carica and other wild species, and goat products. Additionally, marked differences were observed between men and women, which suggest that women consumed a more vegetal-based diet, a finding which is in agreement with the higher proportion of teeth with carious lesions among women.     

Persistent deathplaces and mobile landmarks: The Holocene mortuary and isotopic record from Wadi Takarkori (SW Libya)

Recently, a new program of territorial study was launched in the area of Wadi Takarkori (Wadi Takarkori Project, hereafter WTP), located in south-western Libya at the border with Algeria in a strategic position between the Tassili and Acacus Mountains. The core of this program was the excavation of a rock shelter together with the systematic mapping and selected excavations of stone structures in the wadi area, mainly tumuli, a typical Saharan feature.