Bone preservation,decay and its related conditions in ancient human bones from Kuwait

By means of scanning electron microscopy, four out of a series of twelve inhumations from the Hellenistic Period were examined for traces of the decay process. The changes represented the result of erosion and biological decomposition of human bones on a small island in the northern part of the Persian Gulf over a period of ca. 2200 years. Special emphasis was given to bone preservation and blood cell survival, and to bone changes due to physicochemical erosion and fungus, bacterium, insect and plant-root activity. Related soil and climatic conditions were taken into consideration. The observations should be evaluated to understand the unusual state of preservation of the bones and to avoid possible misinterpretation of pseudo-pathological bone changes as ante-mortem pathology.     

Evaluation of bone strontium as a measure of seafood consumption

The recognition that bone strontium/calcium ratios reflect dietary levels of strontium and that seawater has a high strontium content led some archaeologists to infer that seafood consumption produces high Sr/Ca ratios in bone. Analyses of seawater and of marine organisms reveal, however, a marine trophic effect comparable to the trophic effect seen in terrestrial food chains. This marine trophic effect reduces the Sr/Ca levels in seafood such that marine dietary resources have Sr/Ca levels comparable to those of terrestrial resources. Thus, bone Sr/Ca ratios can not differentiate consumption of marine and terrestrial resources. Also, Sr/Ca of bones from archaeological sites where seafood was an important component of diet were found to be within the range of entirely terrestrial diets. Copyright © 1999 John Wiley & Sons, Ltd.     

Minimum numbers: a consideration

This paper evaluates the various methods used to determine the numbers of individual animals from the numbers of bone fragments found in archaeological sites. It concludes that there is no single “best” method, since the one that is chosen depends upon the result that is desired, and on the particular features of the site and excavation.     

Growth curves of immature bones from a Scottish island population of sixteenth to mid-nineteenth century: Shoulder girdle,ilium, pubis and ischium

Growth curves were prepared for length and various other dimensions, including some innovative ones, of the scapula, clavicle, first rib, ilium, pubis and ischium of ages up to 20 years including several immature and many full-term fetuses. Most curves showed, like the previously reported diaphyses of the limb bones, what appears to be a consistent adolescent growth spurt at about 17 years, but the number of specimens supporting these parts of the curves is too small for the validity of this growth spurt to be beyond doubt. The fetal dimensions were similar to those of Fazekas and Kósa and of other recent populations. For the post-natal stages of growth, no data are available for comparison but there was close correspondence with the growth curves of some ancient peoples, Amerindian, caucasoid and negroid, whose limb-bone growth rates had also been shown previously to be well below those of contemporary caucasoids. Thus it is argued that the shoulder-girdle and hip-bone data provided further evidence that this Scottish island population was affected by stunting factors. Although there is no skeletal evidence of malnutrition, there is plenty of evidence that the way of life was full of hardship in which the children shared. They took a full part in the arduous work involved in farming marginal-quality land; the houses were damp, cold, smoky and dark; they were overcrowded although, mainly because of the high infantmortality rate, families were not large.     

Detection of blood proteins in ancient human bone using ELISA: A comparative study of the survival of IgG and albumin

ABSTRACT The immunological identification of species-specific blood proteins in skeletal remains has an important role in the reconstruction of ancient dietary, ritual and domestic behaviour. However, which protein provides the most suitable target for such work has not been considered previously, and the present investigation was carried out on human bone to assess the relative merits of IgG and albumin. Extracts of bone from 31 individuals (from the English Civil War, medieval, Early Saxon, Roman, Iron Age and Bronze Age periods) were tested by an enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies. Albumin was detected in 23 of the 31 skeletons, including those from the Iron and Bronze Ages, whereas IgG was identified in only one; this difference was very highly significant (P < 0.0005, χ² 30.0). The better detection rate for albumin was thought to be due to its higher original blood level, inherent differences in survival pattern being considered unlikely. Testing the same extract for both proteins in the same assay system ensured that any effects due to soil factors, burial conditions, physical integrity of the bone, chronological age, amount of original specimen, method of analysis and type of reagent were the same for each part of the study, thus permitting a valid comparison of antigen survival to be made. Control samples, including fresh and ancient animal bone extracts and human and animal sera, confirmed that the results were consistent and specific, with no cross-reactivity between human and animal material, and that as little as 10 ng of protein was detectable. In summary, the investigation compared the suitability of IgG and albumin for osteoarchaeological studies using a highly specific, sensitive and versatile ELISA; the results showed that albumin was a far better target molecule for such work and that it can survive in ancient bone for long periods of time.     

Age estimation from the pars basilaris of the fetal and juvenile occipital bone

The pars basilaris of the human occipital bone is reported to be of considerable value for age estimation in juvenile skeletal remains between early fetal periods and 6 years of age, when it fuses to the remainder of the bone. The aim of this investigation was to examine the relationship between various dimensions of the pars basilaris, and assess their reliability for accurate age estimation. The results confirm the usefulness of the bone in this respect and they are discussed in the light of differential growth of the pars basilaris, and in the increase in dimensions of the boundaries of the foramen magnum.     

Number estimation in vertebrate samples

Methods of quantifying bone samples are discussed and the minimum numbers approach criticized. A technique based upon number estimation of game populations is put forward, and some of the practical difficulties of this method are examined. Consideration is given to the underlying statistical assumptions involved in quantified analysis of bone samples, and a method of calculating confidence limits for death population numbers proposed.     

Palaeo‐otology of cholesteatoma

A cholesteatoma is a collection of dead epithelial cells in the ear which becomes infected with low‐grade pathogenic microorganisms. The lesion tends to cause resorption of the surrounding bone, which potentially permits its recognition in skeletal remains. Cholesteatoma may occur in the middle or the outer ear. Using two examples of cholesteatoma in skeletal remains from British archaeological sites, the differential diagnosis of cholesteatoma from other aural diseases is demonstrated. Some of the differences in bony lesions caused by middle ear and external ear canal cholesteatoma which may enable the two to be distinguished in palaeopathological specimens are discussed. Attention is drawn towards the importance of making this distinction in ancient skeletal remains; middle ear and external ear canal cholesteatoma have a different array of causes and very different impacts upon health status. Copyright © 2005 John Wiley & Sons, Ltd.