Ancient DNA in human bones from Neolithic and Bronze Age sites in Greece and Crete

Attempts were made to detect ancient DNA (aDNA) in samples of 88 human skeletons from eight Neolithic and Bronze Age sites in Greece and Crete. Ancient DNA was absent in specimens from Nea Nikomedia, Lerna, Karaviádena (Zakro), Antron Grave Circle A and Mycenae Grave Circle A. For each of three skeletons from Antron Grave Circle B that were sampled, polymerase chain reactions (PCRs) gave products for nuclear but not mitochondrial DNA, but amplicon yield was low and inconsistent with replicate PCRs failing to give reproducible results. With specimens from Mycenae Grave Circle B, evidence for mitochondrial aDNA was obtained for four of the 22 skeletons that were studied, and at Kouphovouno evidence for mitochondrial and/or nuclear aDNA was obtained with eight of the 20 skeletons that were examined. We conclude that, although aDNA might be present in some Eastern Mediterranean skeletons from later centuries of the Bronze Age, it is not commonly found in material from this period and is likely to be absent from older material.     

一部多学科合作的考古著作--读《龙虬庄》

随着考古信息量的增加与研究的深入,对考古报告的编排形式提出了新的要求。《龙虬庄》确立了江淮东部新石器时代化——龙虬庄化,编排形式上体现出化遗物和自然遗物并重的特点。报告对江淮东部古环境、遗址反映的经济形态、人地关系等进行了合理构建和研究。从其所揭示的大量信息来看,《龙虬庄》是一部多学科合作的新体例的考古学作。     

二十世纪有关纸的考古发现不能否定蔡伦发明造纸术（1）

近几十年来,我国考古工作曾先后在一些西汉遗址和墓葬中发现了若干纸状残片,于是引起国内外许多学和广大人民的关注和提出疑问。是否蔡伦前约二百年就有了纸?造纸术还是不是蔡伦发明的?新发掘的纸状物到底是个什么样?经对历次的发掘作了详细的调查研究和分析化验,结论认为：20世纪有关纸的考古发现不能否定蔡伦发明造纸术。     

Cats of the Pharaohs: Genetic Comparison of Egyptian Cat Mummies to their Feline Contemporaries

The ancient Egyptians mummified an abundance of cats during the Late Period (664 - 332 BC). The overlapping morphology and sizes of developing wildcats and domestic cats confounds the identity of mummified cat species. Genetic analyses should support mummy identification and was conducted on two long bones and a mandible of three cats that were mummified by the ancient Egyptians. The mummy DNA was extracted in a dedicated ancient DNA laboratory at the University of California - Davis, then directly sequencing between 246 and 402 bp of the mtDNA control region from each bone. When compared to a dataset of wildcats (Felis silvestris silvestris, F. s. tristrami, and F. chaus) as well as a previously published worldwide dataset of modern domestic cat samples, including Egypt, the DNA evidence suggests the three mummies represent common contemporary domestic cat mitotypes prevalent in modern Egypt and the Middle East. Divergence estimates date the origin of the mummies' mitotypes to between two and 7.5 thousand years prior to their mummification, likely prior to or during Egyptian Predyanstic and Early Dynastic Periods. These data are the first genetic evidence supporting that the ancient Egyptians used domesticated cats, F. s. catus, for votive mummies, and likely implies cats were domesticated prior to extensive mummification of cats.     

Induced metal-ion exchange in excavated human bone

Excavated human bone was exposed to aqueous solutions containing high concentrations of a single added metal ion in order to examine the extent of introduction of contaminating materials during burial. Variables included pH, temperature, ion concentration, state of bone (whole or crushed), structure of buffer, and counterion. Calcium and sodium showed little increase, and even a decrease in some cases. Strontium, zinc, lead, and magnesium showed large increases probably through heteroionic replacement of calcium. Manganese, aluminum, and potassium showed increases, particularly under neutral conditions, probably through infiltration into voids and defects.     

中国古墨与现代墨元素成分研究

对中国古墨（汉墨、明墨与清墨）与不同产地的现代墨元素成分作了对比分析。用离子背散射测定墨中常量元素C、N、O的含量,用质子激发X荧光法测定微量元素K、Ca、Ti、Cu、Fe等10种元素的含量。实验结果表明,古墨中微量元素K、Ca的含量比现代徽墨、沪墨高10倍以上,而现代徽墨与沪墨中元素N和An的含量也有明显的差别。     

历史文化名城的旅游形象设计与实施策略——平遥古城的案例研究

本文在对文脉分析的基础上设计了平遥古城旅游区的一级理念和二级理念、视觉形象和行为形象,提出了40景组合的平遥古城旅游资源形象。     

Morphological study of the ancient dogs from three neolithic sites in China

Ancient Chinese dogs excavated at three archaeological sites and stored in the Institute of Vertebrate Paleontology and Paleoanthropology, Academia Sinica, were the object of this study (Xiawanggang site, 5000–6000 years BP; Keshengzhuang site, 3000–4000 years BP; Shangsunjia site, Bronze age). During these periods, there were large morphological variations in Chinese dogs beyond a single dog class, as with Jomon dogs. Dogs with a large ‘stop’ were already present in China but not in Japan. At the Keshengzhuang site, middle to large class dogs were also excavated. At least three types were confirmed within these dogs. In the aged dog in Xiawanggang, antemortem loss of many teeth was observed suggesting active use of the teeth and it is possible that this dog was used for hunting as were the Japanese Jomon dogs. © 1998 John Wiley & Sons, Ltd.     

郑韩故城出土青铜器的矿料来源初步分析

基于富集在铜料中而保留其母矿信息的元素可以用于示踪青铜器的矿料来源,为了解郑韩故城青铜器的矿料来源,利用ICP-AES方法对郑韩故城出土东周时期青铜器的微量元素进行了检测。结果显示,21个青铜器样品微量元素特征具有相对一致性,推测可能具有相同的原料来源。与小双桥遗址出土青铜器的微量元素特征进行对比,二者存在明显的差异,说明从商代中期到春秋战国时期,这一地区的原料来源发生了改变。研究结果可为研究东周时期这一地区铜业发展状况提供线索。     

Determination of a kinship system using ancient DNA,mortuary practice,and historic records in an upper Canadian pioneer cemetery

Recovered and amplified ancient DNA (aDNA), from a historically documented 19th century Upper Canadian pioneer cemetery produced genotypes that were used to infer a past societal kinship system. While the results from multiplex short tandem repeat (STR) amplifications showed an unreliable polymerase chain reaction (PCR) product, a single locus HUMTH01 analysis yielded reproducible data and an allelic frequency pattern not statistically different from modern populations. Mitochondrial (mt) DNA HVR II data showed that a combined cemetery database exhibited reduced haplotype diversity indicators, as well as clusters of probable maternally related burials. The chronological persistence and replacement of mtDNA clusters approximately every two generations suggests a patrilineal/patrilocal kinship structure from a virilocal burial program for the Harmony Road cemetery. Through the integration of the aDNA analysis with archaeological material culture, historic records, and other ethnohistoric sources of information, this conclusion is supported. In this study persisting patrilineally inherited surnames act as a surrogate for aDNA Y‐chromosome haplotype analysis. These results suggest that aDNA applications on aggregate skeletal collections where sparse, or no ethnological or historical documentation exists, may result in incorrect population history inferences if the presence of a kinship interment bias is not considered. Copyright © 2003 John Wiley & Sons, Ltd.