Inferences from the human skeletal material of the Early Iron Age cemetery at Agios Dimitrios,Fthiotis, Central Greece

The Geometric cemetery of Agios Dimitrios (850–740 B.C.) yielded a human osteological sample, with an MNI of 51 and equal numbers of males and females and adults and subadults. This site is of significant archaeological importance, as it provides information on human health status, diet, and activity patterns as well as mortuary behavior for a little studied time period. The results, including a) stable carbon and nitrogen isotopic data suggesting a C₃ low-protein plant diet, b) relatively high infant mortality, c) low stature estimates, d) significant prevalence of possible anemic conditions, and e) high dental infection and loss rates, all point to a rapidly increasing, stressed, and relatively malnourished population which did not exploit nearby marine resources, experienced suboptimal living conditions, and could not reach its biological potential. Furthermore, the study of this group provides evidence of equal burial treatment of all age and sex categories, no dietary differences in terms of sex and status, and no sex differentiation in pathological conditions.     

Paleodiet in northern Chile through the Holocene: extremely heavy δN values in dental calculus suggest a guano-derived signature?

Dental calculus extracted from the teeth of 28 prehistoric human skeletons from coastal and valley archaeological sites in northern Chile that date from the Archaic period (∼2300 BC) to the Late Intermediate period (AD 1476) was analyzed for stable isotope (δ¹⁵N, δ¹³C) compositions. δ¹⁵N compositions were extremely heavy (+17.8 to +33.1‰), but comparable to some studies using conventional human biomaterials (hair, nail, muscle) at other prehistoric sites in northern Chile. There was a negative correlation between δ¹⁵N and δ¹³C for coastal sites, but a positive correlation for valley sites. Results for the valley sites point to a diet that was influenced by marine resources throughout all time periods. The unusually heavy δ¹⁵N values for the coastal sites require a dietary component with a δ¹⁵N composition significantly heavier than that of marine resources. The hyper-aridity at the study area (mean annual rainfall of 0.5–0.6 mm/year) is a likely contributing factor to the production of heavy δ¹⁵N values, but is unlikely to account for the heaviest δ¹⁵N values (>+30‰). One possible explanation for the heaviest δ¹⁵N values is that dietary components were impacted by isotopically-heavy guano, which is abundant in the region. Guano may have been used as a fertilizer during crop cultivation at the onset of the Formative period, continuing through the Historic period after Spanish contact. The indirect impact of guano from abundant bird habitats in the region may have influenced wild foodstuffs harvested throughout the pre-agricultural period. Results provide support for the utility of dental calculus as a proxy for obtaining stable carbon and nitrogen isotope signatures for use in paleodietary studies.     

High precision isotopic analyses of lead ores from New Mexico by MC-ICP-MS: implications for tracing the production and exchange of Pueblo IV glaze-decorated pottery

Between ca. 1275 and 1700 CE, Pueblo groups in the northern Southwest United States produced and exchanged ceramic bowls decorated with lead-based glaze paints. Previous studies of these glaze-decorated bowls have used lead isotopic analysis by ICP-MS to identify the sources of lead used by Pueblo potters, and investigate how social or economic factors may have influenced resource use among different Pueblo communities (e.g. 13 and 14; Huntley et al., 2007; Huntley, 2008). However, interpretations of much of this isotopic data have remained provisional because of overlap among the isotopic ratios of potential sources and because the isotopic composition of many glaze paints do not clearly match any known source. Here, we use multi-collector ICP-MS to re-measure the lead isotopic composition of 48 samples of lead sulfide (galena) and lead carbonate (cerussite) from sources in New Mexico that were potentially utilized by Pueblo potters, including mines within the Cerrillos Hills, Magdalena, Hansonburg, and Joyita Hills mining districts. These results define the isotopic composition of lead ores from these districts with greater precision and accuracy than achieved in previous studies and better distinguish among these mining districts in lead isotope space. Most significantly, we find that galena mineralization within the Cerrillos Hills only has a modest degree of isotopic variation, with ²⁰⁶Pb/²⁰⁴Pb ratios from 18.508 to 18.753, ²⁰⁷Pb/²⁰⁴Pb ratios from 15.580 to 15.607, and ²⁰⁸Pb/²⁰⁴Pb ratios from 38.388 to 38.560. These ranges are far narrower than previously reported, and should supersede previously published values for this district. In total, we conclude that isotopic measurements of both ores and glaze paints made by MC-ICP-MS will provide new information about the provenance of lead in glaze paints and allow for more detailed interpretations about resource procurement and exchange in the Pueblo world.     

The role of canids in ritual and domestic contexts: new ancient DNA insights from complex hunter–gatherer sites in prehistoric Central California

This study explores the interrelationship between the genus Canis and hunter–gatherers through a case study of prehistoric Native Americans in the San Francisco Bay-Sacramento Delta area. A distinctive aspect of the region's prehistoric record is the interment of canids, variously classified as coyotes, dogs, and wolves. Since these species are difficult to distinguish based solely on morphology, ancient DNA analysis was employed to distinguish species. The DNA study results, the first on canids from archaeological sites in California, are entirely represented by domesticated dogs (including both interments and disarticulated samples from midden deposits). These results, buttressed by stable isotope analyses, provide new insight into the complex interrelationship between humans and canids in both ritual and prosaic contexts, and reveal a more prominent role for dogs than previously envisioned.     

Evidence for “Celtic migrations”? Strontium isotope analysis at the early La Tène (LT B) cemeteries of Nebringen (Germany) and Monte Bibele (Italy)

Strontium isotope analysis on human remains from the Iron Age (4th/3rd century BC) cemeteries of Nebringen, Germany and Monte Bibele, Italy were carried out to investigate the role of residential changes during the period of the historic “Celtic migrations”. From an archaeological perspective, the location of the cemeteries in the Celtic core (Nebringen) and expansion area (Monte Bibele), and the distinctive development of their material culture, suggest that the buried populations had differing mobility rates. On the contrary, the strontium results indicate that only few individuals were mobile or non-local. There is, however, a difference in variation of strontium isotope ratios between the two studied cemeteries, presumably caused by differing geological conditions. In Nebringen changing use of cultivated land in a geologically heterogeneous environment most likely caused varying strontium isotope ratios even within the same jaw, while individual mobility over longer distances can also not be excluded. In Monte Bibele the range of strontium isotope ratios is narrow, and this may be explained by a community living in the same village and using the same agricultural resources. For various prehistoric time periods it has been suggested that some population groups were more mobile than others, for example because they had special social roles. In the two cemeteries studied here, males have slightly more often a non-local birthplace or moved during childhood. In contrast, females had isotope signatures which are more consistent with the local geological environment. Male mobility is, however, not correlated with burial as a warrior, and patrilocal residential patterns were not observed among females. Foreign and local items are found with isotopically local and non-local individuals and objects per se do not indicate descent. The motives for residential changes were, therefore, varied and not constrained to a specific area or population group. The presented dataset provides details on the way of life and land-use in the Celtic communities, for which information is otherwise absent.     

Kinship and mobility in 11th-century A.D. Gammertingen,Germany: an interdisciplinary approach

Excavation in and around a Medieval church in Gammertingen, Germany, revealed the skeletal remains of eight individuals dating to the 10th and 11th century AD. Archaeologists hypothesized that the individuals were the first members of a family later known as the Counts of Gammertingen, a medieval high nobility family. In an interdisciplinary approach, Strontium isotope and ancient DNA techniques were performed in order to test the hypothesis that the church was used as a family burial site and to investigate the provenance of family members. Seven of the eight individuals can be placed in a three-generation genealogy. The isotope analyses establish that the eighth individual had a different birthplace and possibly became a member of the Counts of Gammertingen through marriage. Further, genetic data revealed that distant relatives of the paternal lineage are still present in this area today. Thus, the combined results lead to a very detailed knowledge about a 1000-year old noble family.     

Nursing mothers and feeding bottles: reconstructing breastfeeding and weaning patterns in Greek Byzantine populations (6th–15th centuries AD) using carbon and nitrogen stable isotope ratios

Traditionally, documentary evidence (mainly medical works and the vitae and miracles of saints) served as the primary source of information for perinatal nutrition in the Byzantine era. In the last decade, however, stable carbon and nitrogen isotope ratio analysis has also been applied for the reconstruction of Byzantine breastfeeding and weaning practices. This paper reviews the documentary evidence for Byzantine weaning and compares it to isotopic data for eight Greek Byzantine skeletal samples from the sites of Eleutherna, Kastella, Messene, Sourtara, Stylos, Nemea, Petras and Servia (6th–15th centuries AD). The documentary evidence suggests that Byzantine children were weaned at a relatively late age. The age patterning of the stable isotope data is not as clear as normally seen at single sites. However, the presence of a higher proportion of elevated values in juveniles aged three years or less suggests that weaning was completed by the fourth year—a pattern consistent with the written sources. The data available from this study allow us to make some tentative suggestions about cultural and temporal differences in weaning, and to recommend directions for further research. A comparison of the Byzantine data presented here to data published for Roman-era sites from the Mediterranean and Western Europe suggests that the Byzantines maintained a Roman-era practice of relatively late weaning. In contrast, medieval data for Western Europe and the few measurements made on post-Byzantine Greek material suggest more variation, with some groups weaning late and others weaning earlier, by two years of age.     

Lead isotope analysis as a new method for identifying material culture belonging to the Vázquez de Coronado expedition

Archaeological evidence has become an increasingly important component of efforts to identify the route of the Francisco Vázquez de Coronado expedition through northern Mexico and the southwestern United States (1540-1542). Here, we report the first high-precision lead isotopic measurements of artifacts from two archaeological sites with strong material evidence for the expedition’s presence: Piedras Marcadas Pueblo in New Mexico and the Jimmy Owens Site in Texas. The analysis of lead and copper armaments from both sites reveals that many artifacts have overlapping or extremely similar isotopic ratios. We propose that the narrow range of lead isotopic ratios measured on these artifacts can be interpreted as a geochemical fingerprint for some of the Coronado expedition’s surviving material culture, and provides evidence that we interpret to suggest the expedition derived lead and copper metal from Mexican sources. Such a geochemical fingerprint presents an empirical method for discriminating between artifacts that belonged to the Coronado expedition and those related to subsequent Spanish, historical, or modern activity in the Southwest U.S. Thus, this method could significantly impact the search for and identification of archaeological sites associated with the Coronado expedition.     

Re-investigating fish consumption in Greek antiquity: results from δC and δN analysis from fish bone collagen

One of the frequently encountered issues in ancient Greek dietary reconstructions through isotope analyses has been the apparent unimportance of fish protein in human diets. The significance of this observation is amplified by the abundant ichthyofaunal remains, iconographic evidence and literary information on fish and fishing, pertaining to almost all sites and time periods of Greek antiquity. In this project, we measured for the first time isotopes from a large number of fish bones from Greek sites dating from the Mesolithic to the Classical times, aiming to investigate whether this absence is an artefact of the methodology or whether it reflects a reality of restricted fish consumption. Results show that regional trends are stronger that temporal ones in fish isotope values. The range of values overlaps with terrestrial resources, making it difficult or impossible to reject fish consumption based on isotope data alone. This variability proposes a reconsideration of the amount of fish in ancient Greek diets specifically for each site and amplifies the importance of interdisciplinary studies, especially for regions with variable ecological resources.     

Carbon and nitrogen isotope signals in eel bone collagen from Mesolithic and Neolithic sites in northern Europe

Carbon and nitrogen isotope analysis was undertaken on collagen extracted from eel bone from six Mesolithic and Neolithic sites in northern Europe. The results were compared with data obtained on other archaeological fish remains and modern eel caught in fresh and brackish water and from the sea. The possibility of discriminating between freshwater, brackish and marine signals in archaeological eel bone is evaluated and the implications for archaeology discussed. Our data suggest that eel found at coastal Mesolithic and Neolithic sites have carbon isotope signals consistent with a marine origin with no evidence of freshwater residency. The sample of eel bone from one inland site is small but indicates carbon isotope values more consistent with freshwater residency or at least values intermediate between freshwater and marine carbon pools.