Aspects of ancient Greek trade re-evaluated with amphora DNA evidence

Ancient DNA trapped in the matrices of ceramic transport jars from Mediterranean shipwrecks can reveal the goods traded in the earliest markets. Scholars generally assume that the amphora cargoes of 5th-3rd century B.C. Greek shipwrecks contained wine, or to a much lesser extent olive oil. Remnant DNA inside empty amphoras allows us to test that assumption. We show that short ∼100 nucleotides of ancient DNA can be isolated and analyzed from inside the empty jars from either small amounts of physical scrapings or material captured with non-destructive swabs. Our study material is previously inaccessible Classical/Hellenistic Greek shipwreck amphoras archived at the Ministry of Culture and Tourism Ephorate of Underwater Antiquities in Athens, Greece. Collected DNA samples reveal various combinations of olive, grape, Lamiaceae herbs (mint, rosemary, thyme, oregano, sage), juniper, and terebinth/mastic (genus Pistacia). General DNA targeting analyses also reveal the presence of pine (Pinus), and DNA from Fabaceae (Legume family); Zingiberaceae (Ginger family); and Juglandaceae (Walnut family). Our results demonstrate that amphoras were much more than wine containers. DNA shows that these transport jars contained a wide range of goods, bringing into question long-standing assumptions about amphora use in ancient Greece. Ancient DNA investigations open new research avenues, and will allow accurate reconstruction of ancient diet, medicinal compounds, value-added products, goods brought to market, and food preservation methods.     

Neolithic Hedgehogs (Erinaceus europaeus) from the Island of Gotland show early contacts with the Swedish mainland

Previous research probing early migrations and contacts in the Baltic Sea area is characterized by the analysis of different chronologies and subsistent strategies on all sides of the Sea. Several studies performed on artifact typology, ceramics, grave rituals and physical anthropology ended with varying results. Although the question of human origins remains inconclusive, in this study, we rely on the phylogeography of an animal associated with humans to elucidate findings regarding prehistoric human migration and contacts.Hedgehogs, along with other fauna on Gotland, were brought over to the island by humans. We examined hedgehog mitochondrial DNA from the Pitted Ware Culture (Middle Neolithic). The genetic signatures of the animals on the island were investigated to determine the animal’s origin.From the 23 bones originally examined, twelve bones from all five locations studied yielded reliable results and resembled published extant Erinaceus europaeus sequences from Sweden, Norway and Denmark. We postulate that a western heritage for the Neolithic hedgehogs on Gotland indicates early human contact with the Swedish mainland.     

An Analysis Of Mitochondrial Dna From The Pakhomovskaya Population Of The Late Bronze Age,Western Siberia

This article presents the results of an analysis of mitochondrial DNA extracted from bone samples from Stary Sad – a burial ground representing the eastern variant of the Late Bronze Age Pakhomovskaya culture in the Baraba forest-steppe, Western Siberia. Comparison with mitochondrial DNA data from earlier populations of the region and also with archaeological facts, points to the origins of the Pakhomovskaya people. Certain components of their gene pool were evidently derived from the local pre-Andronovo populations, others from the actual Andronovo (Fedorovka) population and also from later immigrants. In this article an integrative reconstruction based on biological and cultural facts is proposed.     

Molecular and morphological analyses of avian eggshell excavated from a late thirteenth century earth oven

Using ancient DNA (aDNA) extracted from eggshell of the extinct moa (Aves: Dinornithiformes) we determined the species composition and number of eggs found in a late thirteenth century earth oven feature at Wairau Bar (South Island, New Zealand) – one of New Zealand’s most significant archaeological sites. Mitochondrial and nuclear DNA signatures confirmed this oven feature contained fragments of at least 31 moa eggs, representing three moa genera: Emeus; Euryapteryx; Dinornis. We demonstrate through the genetic identification of 127 moa eggshell fragments that thickness is an unreliable character for species assignment. We also present a protocol for assessing the preservation likelihood of DNA in burnt eggshell. This is useful because eggshell fragments found in archaeological contexts have often been thermally modified, and heat significantly increases DNA fragmentation. Eggshell is widely used in radiocarbon dating and stable isotope research, this study showcases how aDNA can also add to our knowledge of eggshell in both archaeological and palaeoecological contexts.     

Species identification of archaeological dung remains: A critical review of potential methods

Abstract

Dung, macroscopically recognisable as such or not, can more commonly be found in archaeological contexts than is perhaps realised. Up to now, identification of dung to the species which produced it is usually either tenuous, or is not possible. However, species identification can be very informative and is necessary before any further studies can be conducted on the dung, for example on health and hygiene in the past and palaeoecology. This study presents a review of potential methods by which species identifications of archaeological dung can be undertaken. Criteria for identification can be divided into three broad categories: morphometric features of the dung; the content of dung and contextual evidence. Overall, the chances of a precise identification are high; however, a combination of different criteria and techniques will often be necessary to establish a secure identification. Moreover, preservation issues may exclude the application of some criteria while several criteria require more research and the expansion of reference collections of recent material. The overall aim is to move towards standardised methods for species identification of archaeological dung.     

DNA FROM HUMAN ANCIENT BACTERIA: A NOVEL SOURCE OF GENETIC EVIDENCE FROM ARCHAEOLOGICAL DENTAL CALCULUS

We report a molecular methodology to obtain and analyse ancient bacterial DNA from archaeological dental calculus. Recent and archaeological DNA samples, as old as 4000 bp , were successfully extracted and amplified with species‐specific PCR primers. We propose this approach in order to: detect the presence of specific bacterial species infecting past human populations; compare the composition of ancient oral microbiomes among human populations; and analyse the genetic variability and covariation of bacteria and human host populations. Genomic analysis of bacteria from dental calculus is a promising source of evidence for palaeopathological and micro‐evolutionary studies, focused either on micro‐organisms or their human hosts.     

DNA analysis in charred grains of naked wheat from several archaeological sites in Spain

In the present work we attempt to recover endogenous ancient DNA from cereal grains preserved under different conditions: charred, partially charred and waterlogged. A total of 126 grains from naked wheat and 18 from barley from different sites on the Eastern Iberian Peninsula ranging from the beginning of agriculture in the region to the turn of the Common Era, were studied. Two different extraction protocols were used, a standard phenol–chloroform method and a silica-based DNA extraction procedure implemented for artificially charred seeds. Amplifications were directed to three markers: the large subunit of ribulose 1,5 biphosphate carboxylase (rbcL) and the microsatellite WCT12 in the chloroplast genome and the x and y subunits of the high molecular weight glutenin gene (Glu-1) in the nucleus. The first two were used to assess the preservation status of the samples, while with the third we tried to identify the wheat grains at species level. It was possible to obtain eleven positive amplifications in 8 partially charred seeds but only two amplifications of the Glu-1 gene from a single sample of the Early Bronze age were genome-specific. Different contamination sources were identified and reported. Cloning and alignment of sequenced clones showed a correspondence of the amplified fragment to modern wheat D genome haplotypes. This result suggests that the sample corresponds to hexaploid wheat (Triticum aestivum L.), thus being the first ancient DNA evidence to date for the cultivation of hexaploid wheat in the prehistoric agriculture of the Iberian Peninsula. Moreover, obtained results highlight contamination problems associated to the study of ancient archaeobotanical charred seeds suggest that the combination of a silica-based extraction method together with the amplification of specific targets is a good strategy for recovering endogenous ancient DNA from this kind of material.     

高光谱图像系统应用于模糊印章的提取研究

传统书画作品中存在有大量模糊不清的印章,对此类印章尚没有很好的手段进行提取和鉴别。为解决这一问题,本工作采用一种用高光谱图像系统采集书画中模糊印章的光谱-图像信息,采用最小噪声分离变换(MNF)处理光谱图像数据,提升了模糊印章的可辨识度。结果表明,采用该方法能够有效的将模糊印章的信息提取出来,有利于印章的鉴别和研究,为书画的文物价值和真伪鉴别的研究提供了科学有效的手段。     

基于光谱成像的彩绘文物新应用与新方法

目前基于光谱成像的彩绘文物分析主要集中在颜料分析、信息增强、隐含信息挖掘等方面,分析方法主要采用遥感图像处理软件。针对文物数据专门分析方法研究尚显不足的问题,提出基于光谱成像的彩绘文物新应用与新方法。主要包括基于最小噪声分离(MNF)变换感知修复区域的新应用,利用感兴趣区域分析与融合挖掘彩绘文物隐含信息以及基于稀疏非负矩阵欠近似彩绘文物线稿提取的新方法。通过在唐墓壁画、彩绘泥塑以及手绘绢画上的实验,发现光谱成像可以有效感知修复区域,提出的新方法可以有效挖掘隐含信息以及提取线稿,对基于光谱成像的彩绘文物分析研究具有重要意义。     

基于可见光谱特征提取的敦煌壁画颜料识别方法研究

为了对壁画颜料种类进行无损识别,根据不同的物质对光的吸收特性不同,决定着光谱反射率不同这一特性,提出一种基于可见光谱的颜料识别方法,并对敦煌壁画所用颜料进行了分析。提取可见光谱曲线二阶导数负数部分表征曲线峰值区域的几何轮廓,利用中值金字塔对提取结果进行三层分辨率表征以突出曲线峰值位置信息,归一化三种表征尺度并组合构建特征空间F,以欧氏距离为准则对壁画采样点与颜料库样本进行相似度判别,从而识别出壁画用颜料信息。结果表明,本研究构建的识别方法能够对壁画颜料种类进行无损识别,在壁画数字存档及修复领域具有一定的应用前景。