Ancient Iberian horses: a method to recover DNA from archaeological samples buried under sub-optimal conditions for preservation

Existing methods to extract, amplify, and sequence ancient DNA (aDNA) from horse bone and teeth were optimized to recover DNA from a depositional environment of highly permeable acidic soil. DNA was successfully retrieved using 0.10g of bone powder from horse (Equus sp.) remains dating to 25 K years utilizing the methods optimized for this archaeological material. The genetic analyses were performed in a facility that is dedicated to ancient DNA research (Paleo-DNA Laboratory, Thunder Bay, ON, Canada) and has not been previously used to analyse modern or ancient horse DNA. Research was replicated to obtain reliable sequencing results for six samples from the Iberian Peninsula that were consistent with published sequences of Equus caballus. The archaeological sequence data obtained support hypotheses that promote the significance that the Iberian Peninsula has had to the multi-focal centres of origin for horse domestication and distribution of modern horse breeds. The data presented may provide evidence of the existence of an Iberian refugium for Equus during the last glacial period, 10 K years BP. Further molecular data analyses will enhance the ideas presented by this data and our understanding of horse domestication and phylogeny. The optimization of molecular techniques to successfully obtain DNA using minimally destructive, cosmetically sensitive techniques from archaeological remains endeavours to foster further cooperation between museums and researchers.     

Digging deeper into the limits of ancient DNA research on syphilis

The search for the origins of syphilis has a long history in the medical and anthropological literatures. If we know more about the emergence of the pathogen that causes the disease in humans we will understand its evolution through time and space as well as shed light on its current state in living populations. Ancient DNA techniques used to isolate Treponema pallidum subsp. pallidum DNA from archaeological human specimens provide direct evidence of its existence in the past. However to date, only Kolman et al. (1999) have been successful in this endeavour, while other attempts have failed (e.g., Barnes and Thomas, 2006; Bouwman and Brown, 2005). Why has there been little success? This paper serves to compliment and add relevant information to Bouwman and Brown's and Barnes and Thomas' discussion concerning our inability to apply ancient DNA techniques to study venereal syphilis in past human populations.Our approach utilized 15 different human specimens from different geographies and different temporal periods: eight samples come from medically diagnosed individuals archived during the American Civil War period; six originate from the United Kingdom and predate 1492 with four of these samples having been previously analyzed by Bouwman and Brown and one sample comes from historic Canada. Human mitochondrial and amelogenin DNA, as well as several genes from the Treponema organism were analyzed revealing the relatively good preservation of human multi-copy and single copy DNA but not treponemal DNA. This study also incorporates a unique molecular experiment using rabbits infected with venereal syphilis to help illustrate that treponemal DNA disseminates to bone early during the first stages of infection but is not present in later stages of the disease using the techniques presented in this study.     

Improved methodology for extraction and amplification of DNA from single grains of charred wheat

Comparisons were made between two commonly used methods for the extraction of ancient DNA from charred plant remains. Using artificially charred wheat seeds, we show that silica-binding is the most efficient method for extraction of DNA. We describe a improved silica-binding procedure, including pre-incubation with N-phenacylthiazolium bromide and increased washing of bound DNA, which yields amplifiable DNA from seeds heated at 200 °C for up to 8 h, conditions which promote the formation of Maillard products which often copurify with aDNA and inhibit subsequent PCRs. We believe that this method will be effective in ancient DNA extraction with most types of charred archaeobotanical material. Both cold- and hot-start PCR procedures gave good amplicon yields with extracts prepared in this way, but cold-start PCRs also resulted in synthesis of short artefact products. Addition of bovine serum albumin to PCRs, an inert carrier substance thought to enhance amplification efficiency by binding contaminants, had no advantageous effect and in fact reduced amplicon synthesis.     

Comparing the survival of osteocalcin and mtDNA in archaeological bone from four European sites

The small mineral-binding bone protein, osteocalcin, has been applied in a number of studies on ancient bone due to predictions of its long-term stability. However, the intact protein has not been shown to survive in ancient bone devoid of DNA, which is a much more phylogenetically informative biomolecule. In this investigation, the survival of osteocalcin is directly compared to the amplification of mtDNA in a set of 34 archaeological samples from four sites throughout Europe. We also present unpublished osteocalcin sequences of seven mammalian species in addition to the 19 published sequences to highlight phylogenetic limitations of this protein. The results indicate that the intact osteocalcin molecule survives less in archaeological samples than mtDNA and is more subject to the temperature of the archaeological site. Amino acid analyses show the persistence of the dominant protein collagen in samples that failed both osteocalcin and mtDNA analyses. The implications these findings present for biomolecular species identification in archaeological and palaeontological material are that, although proteins do survive beyond ancient DNA, osteocalcin does not appear to be the most ideal target.     

古代人骨的分子考古学研究现状与展望

本文重点讨论分子生物学在考古学研究中的应用。根据对人类学研究的回顾与展望,在以研究人类的起源和进化为首要任务的人类学研究领域,由于现代分子生物学理论和方法的应用,为人类学的发展提供了科学可信的研究方法和具发展前景的研究方向。     

古代残留物分析在考古中的应用

动植物是人类社会发展的重要基石,它的利用是人类适应、改造和征服自然的物质基础,古代社会的方方面面都与之相关,因而动植物及其制品的残留物分析能提供古代社会丰富的信息。残留物分析重点在于从残留物中提取有机物。利用科学检测手段进行定性定量分析来判断残留物来源,从而了解古代动植物的加工、利用和相关载体的功能等。本文从DNA、淀粉粒、蛋白质、脂类、炭化物和酒等六个方面简要介绍了残留物分析的方法和进展,希望能促进残留物分析在中国的开展．     

Public archaeology at Baynard-Zion: bringing life back to an antebellum cemetery

ABSTRACT

Heritage tourism is a driving economic force in much of the coastal southeastern United States, including on Hilton Head Island, South Carolina, one of the most popular destinations for vacationers in the country. Working with local community members in developing a diverse and multipronged public archaeology program, we helped facilitate research and develop programing at the Baynard Mausoleum and Zion Chapel of Ease and Cemetery (Baynard-Zion). Built and used during the late eighteenth through mid-nineteenth centuries, Baynard-Zion includes some of the oldest marked graves on the island as well as its oldest standing architecture. Using a constellation of techniques, including geophysical surveys, genetic testing of human remains, and limited excavations, research conducted at Baynard-Zion provides an opportunity to enhance public perception and understanding of pivotal historic events and people on the island while also assisting in development plans that promote heritage tourism.     

旅游目的地品牌基因选择的三力模型构建

旅游目的地竞争已从资源竞争转向品牌竞争。针对当前旅游品牌实践没有价值命题、同质性强等问题，本研究在批判继承现有战略管理、旅游目的地品牌战略等相关理论的基础上，从目的地、客源地、竞争性目的地三个维度，构建了目的地代表力、客源地吸引力、竞争性目的地竞争力的旅游目的地品牌基因筛选的三力（RAC）模型。其中，代表力包括原生性、唯一性、真实性；吸引力包括价值性、自然环境和生活方式差异性、地方依恋性；竞争力包括稀缺性、不可模仿性、难以替代性。旅游目的地品牌基因筛选的三力（RAC）模型将为旅游目的地品牌基因筛选提供完整、详细、具有可操作性且可以广泛应用的定量分析工具，有助于解决当下目的地品牌营销缺乏理论依据问题，能够指导旅游目的地管理机构的品牌建设实践。     

Confirmation of the presence of Mycobacterium tuberculosis complex‐specific DNA in three archaeological specimens

This journal published the first reported identification of Mycobacterium tuberculosis complex (MTB) DNA in ancient human remains but concerns were raised about the article two years after publication. These were based on methodology which, in the field of ancient DNA, was still developing. Here we present a re‐examination of the 1993 research conducted on three specimens which exhibited palaeopathologies indicative of tuberculosis. The specimens were: an ulna from pre‐European‐contact Borneo, a spine from Byzantine Turkey, and a lumbar‐sacral spine from 17th century Scotland. There was insufficient material to permit re‐examination of all of the original samples. The earlier results were confirmed in two independent laboratories using different methodologies. MTB DNA complex‐specific DNA amplicons were obtained, and sequenced in both laboratories, in a re‐analysis of samples which supported the earlier findings. Copyright © 2002 John Wiley & Sons, Ltd.