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The histories of maize in northeastern North America are not well understood at the subregional level. The complexity of formation processes for various lines of evidence for maize use requires the application of many analytical methods and techniques to produce data on subregional maize histories. The present analysis uses bulk δ13C values on directly dated charred encrusted cooking residues to provide the first direct correlation of water-based maize cooking to trends in pottery wall thinning. The results add to the growing body of evidence for the history of maize use in central New York. 相似文献
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Comparisons were made between two commonly used methods for the extraction of ancient DNA from charred plant remains. Using artificially charred wheat seeds, we show that silica-binding is the most efficient method for extraction of DNA. We describe a improved silica-binding procedure, including pre-incubation with N-phenacylthiazolium bromide and increased washing of bound DNA, which yields amplifiable DNA from seeds heated at 200 °C for up to 8 h, conditions which promote the formation of Maillard products which often copurify with aDNA and inhibit subsequent PCRs. We believe that this method will be effective in ancient DNA extraction with most types of charred archaeobotanical material. Both cold- and hot-start PCR procedures gave good amplicon yields with extracts prepared in this way, but cold-start PCRs also resulted in synthesis of short artefact products. Addition of bovine serum albumin to PCRs, an inert carrier substance thought to enhance amplification efficiency by binding contaminants, had no advantageous effect and in fact reduced amplicon synthesis. 相似文献
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ABSTRACTDifferentiating between charred and uncharred plant remains may appear straightforward but for some taxa (here fat-hen, Chenopodium album type) can be very problematic. Recognition of the preservation state is obviously crucial for archaeobotanical data derived from dry, open-air sites. Fat-hen as a common weed, is also one of the most important components of a persistent soil seed bank. It is also a well-known food plant, gathered or cultivated. Numerous findings of fat-hen seeds in unclear states of preservation were noted in the Early Neolithic sites of the Linear Pottery culture in Kuyavia (N Europe). In previous studies such specimens were omitted as probably uncharred. Re-examination of Neolithic finds of fat-hen from that region showed the link of their abundancy with the earliest phases of the Neolithic occupation. The plant probably played an important role in the diet of the early Neolithic settlers there. It may indicate intensive use of local, open, fertile, probably alluvial areas. Distinguishing between ancient and modern specimens of that common weedy plant, producing large amounts of small, black, resistant seeds is thus very important, holding a great potential to shed new light on the origins of agricultural societies in this part of northern Europe. 相似文献
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Problems of sampling carbonized plant material are discussed. Firstly, the problem of actually selecting a sample in the laboratory is considered, and some experiments which investigate various procedures are described. Secondly, the statistical aspects of determining optimal sample sizes are considered. Formulae are given for calculating optimal sample sizes and confidence intervals. Upper bounds, which are independent of the total population size, are provided for the sample size required to achieve any desired accuracy. 相似文献