Funeral practices and foodstuff behaviour: What does eat meat mean? Stable isotope analysis of Middle Neolithic populations in the Languedoc region (France)

The aim of this study is to reconstruct the dietary patterns and economic behaviours of Neolithic populations in the Northwestern Mediterranean using isotopic and archaeological data. Burials come from four sites located in Languedoc–Roussillon region in French Mediterranean area. These sites are dated from the Middle Neolithic period (ca. 4500–3500 BC). They represent the Chasséen culture, characterized by regional features, such as economy management, resulting from territorial control. For this investigation, a stable isotopes (δ¹³C and δ¹⁵N) method has been used on 50 human bone collagens and 28 associated animal bones. This method provides direct dietary information on the protein consumed including the relative amounts of marine vs. terrestrial and animal vs. plant proteins in diets. Isotopic results are mainly compared to archaeological data to understand economic distinctions and potential social status variations between different groups using specific funeral practices, i.e. lithic chamber graves vs. domestic/funeral pits. Results show that individuals buried in lithic chamber graves and those buried in pits did not have the same dietary pattern. This result suggests a possible differentiation between two socio-economic groups, i.e. consumers of resources from herding and from farming. No aquatic food appeared to be routinely consumed by these individuals despite a relative close proximity to sea and freshwater sources. Moreover, these outcomes lead us to hypothesize that: (1) funeral practices could be linked to specific economies and/or (2) to different social status and that (3) burial type and foodstuff could be an expression of religious worship. Further research could include data from other areas, such as Spanish Catalonia where there are funeral structures similar to Languedoc lithic chamber graves.     

Assessing natural variation and the effects of charring,burial and pre-treatment on the stable carbon and nitrogen isotope values of archaeobotanical cereals and pulses

The aim of this study is to assess the potential of charred archaeobotanical cereal grain and pulse seed δ¹³C and δ¹⁵N values to provide evidence of crop growing conditions and as a potential component of palaeodietary studies. In order to reliably interpret archaeobotanical δ¹³C and δ¹⁵N values it is necessary to take into account the impact of charring, burial and laboratory pre-treatment procedures. We examine the effects of charring and burial on bulk δ¹³C, δ¹⁵N, %C, %N and C:N ratios in modern cereal and pulse material, and of cleaning by acid–base–acid (ABA) pre-treatment on modern and archaeobotanical charred material. Our study utilised bulk grain and seed samples to help account for within-ear/pod and between-plant variability in δ¹³C and δ¹⁵N values. Heating at relatively low temperatures and for prolonged times (230 °C for up to 24 h) is conducive to the formation of well preserved, undistorted charred cereal grain and pulse seed. Heating for 24 h has a systematic and predictable effect on δ¹⁵N values, with increases of around 1‰ on average in cereal grains and pulse seeds, and no consistent impact on δ¹³C values. Increases in δ¹⁵N are likely due to the loss of lighter ¹⁴N via N-containing volatiles. Burial (for up to 2 years) and ABA pre-treatment have no significant effects on δ¹³C or δ¹⁵N values. After pre-treatment, however, the %C and %N contents of the archaeobotanical material more closely resembles that of the modern charred grains and seeds, suggesting that archaeobotanical remains accumulate non-structural material during burial but retain their original carbon and nitrogen content. Therefore %C, %N contents and C:N ratios can provide useful criteria for assessing archaeobotanical preservation.     

Moving peoples,changing diets: isotopic differences highlight migration and subsistence changes in the Upper Mun River Valley,Thailand

The dramatic growth of dietary isotope studies in archaeological literature attests to the significant potential this technique has for shedding light on past societies. Human diet reflects complex, inter-linked factors such as status, cultural preferences or environmental constraints on food production. In this study dietary isotope analysis is used to examine the human skeletal remains from Ban Non Wat, northeast Thailand. The study aims to use isotopic data to give insight into patterns of migration and subsistence strategy during prehistory. Ban Non Wat is the most comprehensively excavated site in the Upper Mun River Valley (UMRV), and understanding of prehistoric society in this area is crucial to answering questions of how social complexity arose in the region. Carbon isotope analysis has highlighted migrant individuals invisible to strontium isotope analysis and shown links between unusual burial practice and differences in diet. Results also indicate that diet changed substantially through time, with more reliance on rice in the Bronze Age, correlated with an increase in social differentiation. There is a move away from reliance on rice agriculture in the Iron Age, a time when oxygen isotopes show that environmental conditions were becoming drier, possibly resulting in rice agriculture becoming less viable.     

Stable isotopic evidence of diet in a Greek colonial population from the Black Sea

Archaeological and literary sources indicate that the ancient Greeks relied heavily on terrestrial resources and that their access to certain types of foods varied by sex and status. Human and faunal remains from the Greek colonial site of Apollonia (5th to 2nd century BC) on the Black Sea coast of Bulgaria were analyzed for stable carbon and nitrogen isotopes in order to reconstruct the diet of this population, investigate the relative importance of marine vs. terrestrial resources, and explore variations in diet with respect to age, sex, and burial type. The results of this study indicate that the colonists of Apollonia relied on a mixed diet of terrestrial and marine foods, and that there was little or no variation in diet by age, sex, or burial type. The relationship between δ¹³C and δ¹⁵N data for these samples suggests that while marine foods were an important source of nitrogen, much of the carbon used to construct amino acids came from terrestrial foods.     

Foddering by Mongolian pastoralists is recorded in the stable carbon (δC) and nitrogen (δN) isotopes of caprine dentinal collagen

Direct identification of fodder provisioning in individual animals is a previously unexplored area that has important implications for determining animal husbandry strategies in prehistory. In this study, foddering practices are examined in modern domestic and wild caprines from Mongolia (Baga Gazar'in Chuluu) through carbon and nitrogen analyses of bulk sampled dentin collagen in molar teeth. Foddered caprines display significantly different δ¹³C values compared to non-provisioned caprines. This pattern reflects the consumption of C₄ plants year-round by provisioned caprines while non-provisioned caprines ingest C₄ plants only during the summer months. Provisioned caprines also display significantly different nitrogen isotopic values relative to non-provisioned animals. The difference in nitrogen isotopes between wild and foddered animals may be due to increased access in quality graze through foddering by domestic caprines, which results in improved nutrition and extended lactation periods, or enriched δ¹⁵N of fodder plants relative to background flora. Differences in tooth wear between sampled wild and foddered animals may also contribute to the observed δ¹⁵N differences.     

Variations in the C/C ratios of modern wheat grain, and implications for interpreting data from Bronze Age Assiros Toumba, Greece

Variations in the ¹³C/¹²C ratios of wheat grain at different spatial and temporal scales are examined by analysis of modern samples, including harvests of einkorn and durum wheat from Greece, and serve as a guide to interpreting data for Bronze Age grains from Assiros Toumba. The normal distribution and low variability of δ¹³C values of einkorn from 24 containers in the Assiros storerooms are consistent with pooling of local harvests, but less likely to represent the harvest of several years or include grain imported from further afield. Correlation between emmer and spelt δ¹³C values provides strong support for other evidence that these were grown together as a maslin crop. ¹³C discrimination (Δ) for the Bronze Age samples is estimated to be 2.5‰ larger than at present, and would be consistent with an intensive, horticultural regime of cereal cultivation, possibly involving some watering.     

Palaeoenvironmental and chronological investigations of the Magdalenian sites of Goyet Cave and Trou de Chaleux (Belgium), via stable isotope and radiocarbon analyses of horse skeletal remains

Trou de Chaleux and Goyet are caves situated less than 30 km apart in Belgium that contain stratified Magdalenian artefacts and butchered faunal remains. Published radiocarbon dates suggest that both sites were contemporaneously occupied during to the Late-glacial interstadial. It has previously been suggested that the Trou de Chaleux Late-glacial faunal remains might be slightly older than those at Goyet Cave, and that Trou de Chaleux has two phases of occupation during the Late-glacial interstadial. However, the limited number of radiocarbon dates currently available makes it impossible to determine whether the sites are truly contemporaneous, and the assessment of their absolute chronologies is complicated by a plateau in the calibration curve. In this paper, bone collagen δ¹³C and δ¹⁵N signatures of horse bones from the two sites are used to reconstruct local palaeoenvironments. We hypothesise that if occupations at the two sites were contemporaneous, the horse collagen δ¹³C and δ¹⁵N isotope signatures from both sites will be similar, since comparable carbon and nitrogen isotopic values would reflect similar diets, ecologies and environments. To provide clear dating parameters, new AMS radiocarbon determinations are also presented. Results show that horse collagen δ¹³C and δ¹⁵N isotope signatures at the two sites are different, indicating that the populations were not subject to similar diets, ecologies and environments. The new radiocarbon dates confirm that the horse bones from Trou de Chaleux date to the Late-glacial interstadial, but they also show that in Goyet Cave Horizons 1 and 2, older horse remains dating to ca. 32?000–27?000 ¹⁴C yr BP are mixed with Late-glacial horse remains. The δ¹⁵N values of the Goyet Cave horse bones radiocarbon dated to the Pleniglacial are substantially higher than those that have Late-glacial radiocarbon dates. Therefore, we now hypothesise that the majority of the horse bones isotopically analysed from Goyet Cave Horizon 1 are Pleniglacial in age. Statistical analysis of the radiocarbon dates indicates that the Late-glacial occupation at the two sites was contemporaneous, and that while there may appear to be two phases of occupation at Trou de Chaleux, this may be an artefact of the radiocarbon calibration curve.     

Wari’s imperial influence on local Nasca diet: The stable isotope evidence

The highland Wari Empire established a presence within the Nasca region of south coastal Peru during the Middle Horizon period. To clarify the nature of this interaction, we analyzed stable carbon and nitrogen isotope ratios of human bone collagen from individuals living in the Southern Nasca Region (SNR) before (1–750 AD) and during (750–1000 AD) imperial influence. The stable isotope data do not indicate that the Wari Empire transformed maize agricultural labor in the Las Trancas Valley of the SNR. In fact, during both time periods, Nasca people had access to a wide range of food items.     

Manganese Black Pigments in Prehistoric Paintings: the Case of the Black Frieze of Pech Merle (France)

Different black pigments of natural origin (mineral or organic) have been examined by means of several analytical methods (colour measurements, elemental analysis and structural analysis). The results, after being compared with each other, then served as reference points in the study of the ‘Black Frieze’ of the cave of Pech Merle (Lot). After that, colour measurement was investigated in situ on the paintings as a means of displaying small differences (in hue or chroma) between the black colours. The aim of this study was to verify several hypotheses concerning the techniques used by the painters of Pech Merle, and specifically by the one(s) of the Black Frieze. A first objective was that of identifying the nature and, if possible, the origin of the black pigments used in these figures. A second objective was that of determining in which parts of the frieze one or the other (or one and the other) had been used; and the final objective was to provide new technical information that might help us better to understand how the Pech Merle frieze was produced, whether by a single painter and in one episode, for the most part, or, on the contrary, in several episodes and by a succession of different painters.     

大同南郊北魏墓群人骨的稳定同位素分析

探索中原地区农耕文明对游牧民族的影响,对于揭示游牧民族的汉化过程、了解民族间的相互交流和融合具有非常重要的意义。本文对山西大同南郊北魏(拓跋鲜卑)墓群出土的人骨进行了C、N稳定同位素分析,旨在揭示其食物结构的基础上探讨农耕文化对其生活方式的影响。一至四期出土人骨的δ13C值为-9.12±0.85‰,表明其主要以C4类食物为主,而其δ15N值(9.56±0.76‰)甚高,显示先民曾摄取大量肉食,即拓跋鲜卑进入中原地区之后,似乎仍以畜牧业为主,而受中原地区农耕文明的影响甚小。至于δ13C和15N异常的四个样品,可能来自其他地区。结合考古资料和相关文献,不难发现,与慕容鲜卑相比,拓跋鲜卑的汉化之路更为艰难。