Strontium isotope evidence of migration and diet in relation to ritual tooth ablation: a case study from the Inariyama Jomon site,Japan

Ritual tooth ablation was extensively practiced among Jomon (Japanese Neolithic) societies in their final phase (ca. 3000-2300 BP). This tradition includes two different tooth ablation patterns, type 4I and type 2C, referring to extraction of the mandibular incisors and canines, respectively. However, the reason for this difference is unclear. Previous carbon and nitrogen stable isotope analysis of human remains from the Inariyama shell mound revealed that type 4I individuals were more dependent on terrestrial resources and type 2C individuals on marine resources. To test this hypothesis, we performed strontium (Sr) isotope (⁸⁷Sr/⁸⁶Sr) analyses on the same skeletal remains and on modern plants around the site. Because Sr isotope ratios of plants differ according to the local geology and seawater has a consistent Sr isotope ratio, the Sr isotope ratios of tooth enamel can reveal both migration and diet. Comparing Sr isotope ratios in plants and seawater with those of tooth enamel, we identified four possible immigrants. Type 4I locals had significantly higher Sr isotope ratios than type 2C locals. The ratios of the type 4I and type 2C locals were close to those of terrestrial plants and seawater, respectively, suggesting that type 4I locals had incorporated much Sr from terrestrial resources and type 2C locals from marine resources. These results support the hypothesis that ritual tooth ablation reflects dietary differences throughout an individual’s life, and they suggest possible occupational differentiation among the Jomon people.     

Stable isotopes and diet at Ancient Kerma,Upper Nubia (Sudan)

Carbon and nitrogen stable isotope values were measured from bone collagen extracted from archaeological Nubian human (n = 54) and faunal (n = 61) populations from the site of Kerma, Sudan. Collagen suitable for isotopic analysis was extracted from 22 faunal and 48 human samples from the Eastern cemetery site, dated to the Middle Kerma (c. 2050–1750 BC) and Classic Kerma (c. 1750–1500 BC) periods respectively. The isotopic data indicate that the human dietary regimen included a mix of C₃ and C₄ plant-derived components, with a larger C₄ component than previously reported in archaeological Egyptian Nile Valley populations. Elevated δ¹⁵N values are attributed to consumption of dietary resources from a ¹⁵N-enriched terrestrial ecosystem. The faunal isotope data also indicate the consumption of C₃ and C₄ plants. The large range of δ¹³C values measured in both the human and faunal samples supports previous work suggesting that a significant portion of the populations buried at Kerma may have originated elsewhere, further confirming the Nile Valley as a major corridor for population movement in the region.     

Stable carbon- and nitrogen-isotope ratios and electron spin resonance (ESR) g-values of charred bones: changes with heating and a critical evaluation of the utility of g-values for reconstructing thermal history and original isotope ratios

Changes in the stable carbon and nitrogen isotope ratios of modern bone samples heated to a variety of times and temperatures were used to determine the effect of heating on isotope ratios and the retention of organic matter in charred bones. For organic extracts produced by slow demineralization in weak acid, δ¹³C values were unchanged, while δ¹⁵N values increased by up to 5‰ and were primarily determined by heating temperature. Changes in the electron spin resonance (ESR) g-value of whole bone and organic extracts were also measured. For organic extracts from charred bones, the g-value was well-correlated with δ¹⁵N and temperature, suggesting that g-values could be used to estimate the charring temperature and original δ¹⁵N values of charred bones. Thus, g-values from demineralized extracts could be very useful in forensic investigations where it would important to reconstruct the thermal history of burned bones. Isotope ratios and g-values of demineralized extracts from four prehistoric components at three sites that produced cremated human bone were used to test whether the same approach can be applied to archaeological materials. While carbon isotope ratios of the prehistoric samples were similar to those of uncharred specimens, nitrogen isotope ratios were increased and the g-value corrections for nitrogen isotope ratios were not effective.     

Stable isotopic analysis of human bones from Jiahu site,Henan, China: implications for the transition to agriculture

We have investigated change in subsistence during the transition to agriculture in the site of Jiahu, Henan Province, China, using stable isotopic analysis of collagen and apatite in human bones. Millet agriculture is well documented at drier high latitudes of the Yellow River Valley, while rice agriculture predominated at wetter lower latitudes of the Yangtze Valley region. The early Neolithic site of Jiahu lies near the boundary between the drier north and wetter south. Archaeobotanical evidence shows that rice was a significant component of diet at Jiahu, but its δ¹³C value is similar to that of other foods, and therefore cannot be conclusively identified by carbon isotope analysis. Foxtail and broomcorn millets are the only C₄ crops known for the Chinese Neolithic. Because of their high δ¹³C values, their consumption can be evaluated by stable carbon isotope analysis of human bone. Collagen reflects mainly the δ¹³C value of dietary protein, and apatite accurately records that of the whole diet. Isotopic analysis of 15 well-preserved samples from three periods shows that collagen δ¹³C values were very low for almost all individuals, suggesting C₃-based foods dominated their diets. However, apatite carbonate δ¹³C values and δ¹³C spacing between collagen and apatite (Δ¹³C_ap-co) indicate that millet may have been a minor component of the diet in this region. Individuals, who consumed the smallest amounts of animal protein, as indicated by low δ¹⁵N, generally had the highest apatite δ¹³C values. Archaeobotanical evidence for millet at Jiahu is needed to support this interpretation.     

New light on early caprine herding strategies from isotope analysis: a case study from Neolithic Anatolia

The measurement of stable carbon (δ¹³C) and nitrogen (δ¹⁵N) isotopes of caprine bone collagen from the Neolithic sites of Çatalhöyük and Aşıklı Höyük in south-central Anatolia have allowed examination of exploitation and herding practices of sheep and goats. The isotope values from protodomestic caprines at Aşıklı Höyük suggests that these animals were consuming very similar foods to each other and were all confined to the same or similar environments with no access to C₄ plants. At Çatalhöyük, the results show how the caprine management strategy develops from the strategy seen at Aşıklı Höyük into a more varied practice at an early stage as the site grows with an increasing dietary contribution obtained from C₄ plants. No change in diet is isotopically discernible at Aşıklı Höyük. Interestingly, no distinction could be made between the diets of sheep and goats at either site. Therefore, such studies are a useful method of examining the development of early herding or management strategies of caprines in the Near East.     

Stable carbon and nitrogen isotope variations in tooth dentine serial sections from Wharram Percy

Here we report δ¹³C and δ¹⁵N measurements of serial sections of human deciduous and permanent tooth dentine from archaeological samples taken from the medieval village site of Wharram Percy, Yorkshire, UK. We found a pattern of enrichment, for both δ¹³C and δ¹⁵N, where the tooth crown was greater than the cervical part of the root, which in turn was greater than the apical portion of the root and the associated rib collagen values. This pattern reflects a decrease in the consumption of isotopically enriched breast milk and the introduction of less enriched weaning foods in the diet. The (mean±SD) difference between the deciduous second molar crowns and corresponding rib samples from the same individuals after 2 years of age was 1.2±0.4‰ for δ¹³C and 3.2±0.8‰ for δ¹⁵N. The δ¹⁵N values are as predicted, but as there were no C₄plants at Wharram Percy, this 1.2‰ enrichment in δ¹³C represents clear evidence of a carbon trophic level effect in collagen from breastfeeding infants. Carbon and nitrogen results also show that the infant diet among those who died in infancy did not differ from those who survived into childhood. This study demonstrates the promise of using dentine serial sections to study the temporal relationships of breastfeeding, weaning, and dietary patterns of single individuals.     

Lapita subsistence strategies and food consumption patterns in the community of Teouma (Efate,Vanuatu)

The subsistence strategies of the Lapita populations (3100–2800 BP), the first colonisers of the pristine environments of the islands of Eastern Melanesia and Western Polynesia, have been a matter of ongoing debate for decades. Opinions have ranged between the two extremes of Lapita colonisers being either characterised as highly mobile foragers to fully horticultural communities. To further address the question, this paper presents stable carbon and nitrogen isotopic data obtained from analyses of human and animal collagen samples from the site of Teouma (Efate, Vanuatu) dated to between c. 3000–2500 BP. The isotopic signatures obtained from 28 samples (23 human and 5 animal), interpreted in combination with isotopic information from several coastal and insular environments, suggest a diet primarily made up of terrestrially derived animal protein with lesser contributions from vegetable produce and inshore marine species. Comparisons linking the isotopic data gleaned from the Teouma individuals and Lapita subsistence patterns reconstructed through archaeozoological and archaeobotanical remains support the hypothesis of a mixed economy, that included terrestrial foraging, inshore marine exploitation and a low level of food production for at least some of the earliest Lapita colonists in Vanuatu.     

FOCUS: effect of diet and protein source on carbon stable isotope ratios in collagen: follow up to

We analyzed carbon stable isotope data from bone collagen of animals consuming varied experimental diets, including recently published data from Warinner and Tuross [Warinner, C., Tuross, N., 2009. Alkaline cooking and stable isotope tissue–diet spacing in swine: archaeological implications. Journal of Archaeological Science 36, 1690–1697; this journal]. Comparing regression lines for the relationship between collagen and diet δ¹³C, we show that protein source, and not physiology, explains the apparent taxonomic difference between swine and rodents reported in that paper. Our results reveal a complex relationship between whole diet and dietary protein in determining collagen δ¹³C values, such that in many cases, collagen alone may not provide reliable reconstructions of paleodiet. We advocate the simultaneous use of both collagen and apatite δ¹³C, whenever possible, to assess the diets of prehistoric peoples.     

A palaeodietary investigation of carbon (C/C) and nitrogen (N/N) in human and faunal bones from the Copper Age cemeteries of Varna I and Durankulak,Bulgaria

Stable isotope analyses have been applied to human and faunal bone collagen from the Varna I and Durankulak cemeteries to explore palaeodietary adaptations in the Neolithic and Eneolithic (Copper Age). The results suggest both populations primarily utilised terrestrial, C₃-based diets, despite their proximity to the Black Sea. The wider δ¹⁵N range of the Durankulak humans likely indicates the differential utilisation of terrestrial meat sources, which is probably related to the degree to which primary and/or secondary ovicaprid products were consumed, particularly since ovicaprid δ¹⁵N values differ from other herbivores. The isotopic distribution of Varna I reflects a linear relationship between δ¹⁵N and δ¹³C, suggesting that a minority of individuals enriched in both isotopic parameters supplemented their diets with marine resources. These burials include the well known ‘chieftain’ (burial 43) and show notable material wealth by way of grave goods. At the population level, however, there is no significant correlation between stable isotope values and material wealth at Varna I, a fact with implications for theories regarding emergent social/economic hierarchies in Balkan prehistory. Five burials at Durankulak were found to have relatively enriched δ¹³C and δ¹⁵N values with respect to the rest of the population. These burials reflect a prominently marine-based or mixed terrestrial C₃-based diet that included C₄ inputs, possibly from millet, for which the limitations of stable isotope analysis on bulk collagen are not able to differentiate. AMS dating has shown that these burials belong to a much later period.     

Probing dietary change of the Kwädąy Dän Ts'ìnchį individual,an ancient glacier body from British Columbia: II. Deconvoluting whole skin and bone collagen δC values via carbon isotope analysis of individual amino acids

The remains of the Kwäd?y Dän Ts'ìnch? individual, a frozen male human, were recovered from a retreating glacier within the Tatshenshini-Alsek Park in British Columbia in August 1999. In order to provide information on both the geographical origin of this individual and low long he spent in the remote interior region prior to his death, molecular analysis and compound-specific carbon isotope analyses were performed on individual amino acids purified from his skin and bone. Gas chromatographic quantification of constituent amino acids of both tissues revealed a molecular distribution characteristic of collagen, dominated by glycine and to a lesser extent proline, hydroxyproline and alanine. Chiral gas chromatography indicated that protein preservation in both tissues was exceptional. Carbon isotope analysis of a faunal assemblage from an earlier prehistoric site from southern British Columbia provided reference dietary amino acid δ¹³C values for terrestrial (deer and domestic dog) and marine species (salmon and sealion), showing clear separation in all amino acids, particularly glycine which was extremely ¹³C-enriched in the marine animals. The distinction between terrestrial and marine organisms was increased by exploring Δ¹³C_{Glycine-Phenylalanine} values (6.6 ± 0.6‰ and 15.0 ± 2.1‰, respectively), which were higher in the latter by approximately 8‰, mirroring the increased δ¹⁵N_{Bulk collagen} values observed for the marine animals (R² = 0.78; p < 0.001). The Kwäd?y Dän Ts'ìnch? individual's bone had a similarly elevated Δ¹³C_{Glycine-Phenylalanine} value of 15.6 ± 1.0‰, indicating his extreme reliance on marine dietary resources throughout early life. The skin amino acid δ¹³C values were consistently lower than those observed for bone, with a concurrently lower Δ¹³C_{Glycine-Phenylalanine} value of 12.7 ± 0.9‰. The shift between the carbon isotope composition of bone (long-term diet) and skin amino acids (short-term diet) confirmed a sudden divergence away from marine food sources in the last months of life, consistent with his discovery 80 km inland.