Examining histology to identify burned bone

Early research suggests that examination of the microscopic internal structure is a valid method for distinguishing burned from unburned bone in the archaeological record. This study compares burned and unburned modern bones with archaeological bones from Sibudu Cave (ca. 60,000 years ago) and Cave of Hearths (over 200,000 years ago) to more fully describe the heat-induced histological changes to bone. We also explore the effects of diagenesis on those changes to determine if histological evidence of burning preserves through fossilisation and diagenetic processes and can be successfully used to identify ancient burning. Application of this technique can help in addressing various issues, including the origins of controlled use of fire and understanding animal butchery and disposal.     

Tending and drive hunting: A density-mediated attrition model can explain age profiles of white-tailed deer at Iroquoian village sites

In a 1993 paper, Noble and Crerar suggested, based on the age and sex distribution of white-tailed deer (Odocoileus virginianus) longbones from four Attawandaron Iroquoian sites around the western end of Lake Ontario, that the contact-period Attawandaron were managing local deer populations, in response to the aboriginal trade in hides. Having observed similar patterns of differential preservation of elements and epiphyseal fusion at nearby Iroquoian sites that are not ascribed to the Attawandaron, and which pre-date the hypothesised trading period, we argue that these patterns may instead relate to bone density and/or vulnerability to taphonomic effects of late-fusing epiphyses. We further support our argument through age profiles based on dental eruption and wear.     

Investigating the potential of micro-focus computed tomography in the study of ancient bone tool function: results from actualistic experiments

Many experiments have sought to recreate the types of damage that would be expected in ancient stone and bone weapon tips. This damage is usually presented as visible fractures or microscopic surface modification. Fatigue tests conducted on bovine bones, however, show the development of internal micro-cracks that result from stress, prior to actual breakage. In this paper I present the results of an experimental investigation of bone points subjected to a variety of activities. I assess the presence of microdamage using micro-focus computed tomography. The results show that two patterns of micro-cracks develop in bone and are best viewed in longitudinal section. Micro-cracks are a cumulative feature dependent on the amount of load applied and the duration of activity. When subjected to high enough loading rates, micro-cracks will merge together to eventually form a fracture. Although further tests are needed to confirm the exact point at which these fatigue fractures begin to form, micro-focus computed tomography has the potential to reveal whether an individual bone point underwent multiple or prolonged impacts and thus to elucidate the probable function/s of ancient pointed bone tools where no visible damage is apparent. Micro-focus computed tomography is a non-destructive and non-invasive procedure and therefore safe to use on archaeological artefacts.     

Late Pleistocene Crocuta crocuta spelaea (Goldfuss, 1823) clans as prezewalski horse hunters and woolly rhinoceros scavengers at the open air commuting den and contemporary Neanderthal camp site Westeregeln (central Germany)

Late Pleistocene Ice Age Crocuta crocuta spelaea ( Goldfuss, 1823) hyenas from the open-air gypsum karst site Westeregeln (Saxony-Anhalt, central Germany) is dated into the early to middle Late Pleistocene. Hyena clans apparently used the karst for food storage and as “commuting den”, where typical high amounts (15% of the NISP) of hyena remains appear, also faecal pellets in concentrations for den marking purposes. Additionally small carnivores Meles, Vulpes and Mustela appear to have used some cavities as dens. Several hundreds of lowland “mammoth steppe fauna” bones (NISP = 572) must have been accumulated primarily by hyenas, and not by Neanderthals at the contemporary hyena/human camp site. Abundant caballoid horse remains of “E. germanicus Nehring, (1884)” are revised by the holotype and original material to the small E. c. przewalskii horse. Woolly rhinoceros Coelodonta antiquitatis remains are also abundant, and were left in several cases with typical hyena scavenging damages. Rangifer tarandus (11%) is mainly represented by numerous fragments of shed female antlers that were apparently gathered by humans, and antler bases from male animals that were collected and chewed in few cases (only large male antlers) by hyenas. The large quantities of small reindeer antlers must have been the result of collection by humans; their stratigraphic context is unclear but such large quantities most probably resulted from schamanic activities. The hyena site overlaps with a Middle Palaeolithic Neanderthal camp, as well as possibly with a later human Magdalénian site.     

Changes in human bones boiled in seawater

The differences between boiled or unboiled bones are not often studied. However, they are crucial to understand postmortem rituals and to establish defleshing procedures and mortuary practices. In this work, human bones boiled in sea or fresh water are characterized. The bone composition, as well as the compounds present in the resulting materials, shows that salt alters the boiling process mechanism. Hence, from structural and morphological criteria, it is possible to distinguish if a bone has been boiled in salt or fresh water. In both sets of samples, the smoothness of the bone surface depends on boiling time, but only in bones boiled in seawater, filaments are observed apparently pouring out of the pores.Those differences which are mainly morphological (smoothness of the surface) are explained in terms of a collagen diffusional mechanism favored by sodium and chloride ions. For a boiling time of 6 h, the surface is covered by a thick layer or crusts of degraded collagen. Experiments with seawater may be used as model experiments to simulate taphonomical alterations in bones exposed to salt water.     

The Mousterian bone retouchers of Noisetier Cave: experimentation and identification of marks

Retouchers are fragments of bone used during the Paleolithic to strike stone flakes in order to transform them into retouched tools. Our experiments show that the mark produced on retouchers differs depending on whether they were used to strike flint or quartzite. Our results suggest that numerous pits, most often with an ovoid form, characterize the retouching of quartzite flakes. Most of the scores produced with this material have a sinuous morphology with rough interior faces. The areas with superposed traces have a pitted appearance. On the other hand, the retouching of flint flakes, produces pits that are most often triangular in form. Most of the scores have a rectilinear morphology with smooth interior faces and their superposition results in the formation of hatch marks. There is also a relationship between the characteristics of the mark and the relative state of freshness of the retouchers. The validity of the criteria identified was confirmed by a blind test. These diagnostic criteria were applied to archaeological retouchers from the Mousterian site of Noisetier Cave. The results obtained improve our knowledge of the technical behaviors of Neanderthals and allow us to address questions concerning their techno-economic implications.     

Establishing collagen quality criteria for sulphur isotope analysis of archaeological bone collagen

Sulphur isotope measurements of bone collagen from archaeological sites are beginning to be applied more often, yet there are no clear criteria to assess the quality of the collagen and therefore the validity of the sulphur isotope values. We provide elemental data from different methods (DNA sequences, amino acid sequences and mass spectrometric measurements) which are used to establish a reliable system of quality criteria for sulphur isotope analyses of bone collagen. The difference in the amount of sulphur from fish and mammalian collagen type I led to the suggestion to use different criteria to assess the in vivo character of the collagen between these two categories. For establishing quality ranges, the bone collagen of 140 modern animals were analysed. The amount of sulphur in fish and mammalian bone collagen is 0.63 ± 0.08% and 0.28 ± 0.07%, respectively. Based on these results we define for mammalian bone collagen an atomic C:S ratio of 600 ± 300 and an atomic N:S ratio of 200 ± 100, and for fish bone an atomic C:S ratio of 175 ± 50 and an atomic N:S ratio of 60 ± 20. These quality criteria were then applied to 305 specimens from different archaeological contexts.     

The sinking of the Mary Rose warship: a medieval mystery solved?

The cause of the sinking of Henry VIII's Vice Flagship, the Mary Rose, as she sailed out to meet the French fleet on 19th July AD 1545, has remained an enduring mystery and contested encounter between the English and French Navies. The French claim was that the ship was holed by French cannon fire, whilst the English maintained that she sank due to a poorly executed navigational manoeuvre during the engagement. On the day of the sinking there was a total listed crew complement of 415 men onboard. Minimum number analysis of the commingled human assemblage identified only 179, from which we sampled 18 individuals from differing decks within the ship. We measured δ¹³C and δ¹⁵N in bone collagen, and δ¹⁸O and δ¹³C in enamel apatite of these individuals in order to obtain information about their diets and origins. While the collagen δ¹³C and δ¹⁵N data are similar to other medieval populations, the δ¹⁸O data indicate that a significant proportion of the crew did not originate in Britain, but rather they emanated from warmer, more southerly, regions. These data suggest the presence of 33–60% of non-natives, possibly mercenaries and/or ‘prest’ men, amongst the crew. Together with the contemporary remark shouted from the Mary Rose to a passing ship, that the Captain had the “type of knaves of whom, he could not rule”, our results lend weight to the suggestion that poor communication may well have contributed the observed fatal navigational manoeuvre which led to her sinking.     

Probing dietary change of the Kwädąy Dän Ts'ìnchį individual,an ancient glacier body from British Columbia: II. Deconvoluting whole skin and bone collagen δC values via carbon isotope analysis of individual amino acids

The remains of the Kwäd?y Dän Ts'ìnch? individual, a frozen male human, were recovered from a retreating glacier within the Tatshenshini-Alsek Park in British Columbia in August 1999. In order to provide information on both the geographical origin of this individual and low long he spent in the remote interior region prior to his death, molecular analysis and compound-specific carbon isotope analyses were performed on individual amino acids purified from his skin and bone. Gas chromatographic quantification of constituent amino acids of both tissues revealed a molecular distribution characteristic of collagen, dominated by glycine and to a lesser extent proline, hydroxyproline and alanine. Chiral gas chromatography indicated that protein preservation in both tissues was exceptional. Carbon isotope analysis of a faunal assemblage from an earlier prehistoric site from southern British Columbia provided reference dietary amino acid δ¹³C values for terrestrial (deer and domestic dog) and marine species (salmon and sealion), showing clear separation in all amino acids, particularly glycine which was extremely ¹³C-enriched in the marine animals. The distinction between terrestrial and marine organisms was increased by exploring Δ¹³C_{Glycine-Phenylalanine} values (6.6 ± 0.6‰ and 15.0 ± 2.1‰, respectively), which were higher in the latter by approximately 8‰, mirroring the increased δ¹⁵N_{Bulk collagen} values observed for the marine animals (R² = 0.78; p < 0.001). The Kwäd?y Dän Ts'ìnch? individual's bone had a similarly elevated Δ¹³C_{Glycine-Phenylalanine} value of 15.6 ± 1.0‰, indicating his extreme reliance on marine dietary resources throughout early life. The skin amino acid δ¹³C values were consistently lower than those observed for bone, with a concurrently lower Δ¹³C_{Glycine-Phenylalanine} value of 12.7 ± 0.9‰. The shift between the carbon isotope composition of bone (long-term diet) and skin amino acids (short-term diet) confirmed a sudden divergence away from marine food sources in the last months of life, consistent with his discovery 80 km inland.     

Variation in strontium isotope ratios of archaeological fauna in the Midwestern United States: a preliminary study

Strontium isotope values (⁸⁷Sr/⁸⁶Sr) in bone and tooth enamel have been used increasingly to identify non-local individuals within prehistoric human populations worldwide. Archaeological research in the Midwestern United States has increasingly highlighted the role of population movement in affecting interregional cultural change. However, the comparatively low level of geologic variation in the Midwestern United States might suggest a corresponding low level of strontium variation, and calls into question the sensitivity of strontium isotopes to identify non-local individuals in this region. Using strontium isotopes of archaeological fauna, we explore the degree of variability in strontium ratios across this region. Our results demonstrate measurable variation in strontium ratios and indicate the potential of strontium analysis for addressing questions of origin and population movement in the Midwestern United States.