Stable isotope analysis of Late Upper Palaeolithic human and faunal remains from Grotta del Romito (Cosenza), Italy

Stable isotope analysis of carbon (δ¹³C), nitrogen (δ¹⁵N) and sulphur (δ³⁴S) were carried out on one of the largest assemblages of Late Upper Palaeolithic human remains in Southern Europe, at Grotta del Romito (Cosenza), Italy. The burials were stratigraphically dated from ca. 18,000 to 13,000 cal BP, which was confirmed by a series of new AMS dates made directly on the bone collagen. Dietary reconstruction from carbon and nitrogen stable isotopes revealed that eight of the nine individuals analysed, dating to the Final Epigravettian, had very consistent diets, rich in terrestrial animal protein, regardless of their age or sex. These included two individuals who were suffering from severe pathologies. A single individual, dating to the Evolved Epigravettian had a more variable diet, which was significantly enriched in protein from marine or freshwater fish compared to the later burials. Overall, the results are consistent with the very limited number of other studies which describe a change to more specialised and less variable subsistence strategies, in this case the hunting of large herbivores, towards the end of the Palaeolithic period. Sulphur isotope values of all of the nine burials and several faunal samples were notably consistent, showing no evidence of long-distance migration to the site from a different geological zone.     

Intrataxonomic variability in metapodial and femur bone density related to age in guanaco (Lama guanicoe). Zooarchaeological and taphonomical implications

The aim of this paper is to present new information about bone mineral density (BMD) variability in guanaco (Lama guanicoe), a South American wild camelid; while taking into account its ontogenic development. The importance of considering intrataxonomic variability in bone density related to age for constructing zooarchaeological indices and applying them to archaeological assemblages is also discussed. The results presented here were developed from a large sample of 46 metacarpals and 45 femurs of modern guanacos, ranging in age from ca. 6 months of gestation to 12 years. Bone structural densities were measured using a biphotonic X-ray densitometer, DEXA method, Norland EXCELL and the bone mineral content (BMC) of each scanned portion or complete element was divided by its volume, which was obtained independently by water displacement. Our results indicate that the bone density of each anatomical unit is highly variable, even among individuals of the same species. According to metacarpals data, age is an important factor affecting BMD. Moreover, our study shows that the density ranges of different skeletal elements can overlap, suggesting that it is not possible to predict that a certain element is always denser than another one. Finally, we present new evidence about the potential age-related taphonomic bias in faunal assemblages, indicating that meanwhile some skeletal elements show clear differences in their density between their fused and unfused state (metacarpal) other do not (femur).     

Diagnostic morphometric applicability of confocal laser scanning microscopy in Osteoarchaeology

The confocal laser scanning microscope (CLSM) has become an essential tool for a wide range of biological and clinical studies and imaging applications. The major imaging modes of the CLSM include single and serial optical sections from thick specimens (100 µm), multiple wavelength images and three‐dimensional reconstruction. Such images provide measurement of length, surface or volume of objects. Surprisingly, this advanced microscopic method has been very rarely used in anthropological research, although numerous clinical and basic science research projects showed its value to study, bone growth, bone micro‐architecture and 3D bone morphometry. We present the basic principles and advantages of CLSM and outline practical aspects of specimen preparation, image collection and digital image processing specifically appropriate for historical bone and hair material. Copyright © 2009 John Wiley & Sons, Ltd.     

Experimental chemical degradation compared to natural diagenetic alteration of collagen: implications for collagen quality indicators for stable isotope analysis

Stable isotopic ratios from archaeological bone collagen are valid palaeodietary indicators, but depend on sufficiently well preserved molecules and several collagen quality criteria have to be fulfilled (mostly collagen wt%, C%; N%, C/N molar ratio). For a reassessment of these quality criteria, and a better understanding of the chemical degradation of bone collagen, experimentally degraded modern bones and 54 archaeological human bones were investigated. In the course of the experimental degradation, alterations of isotopic ratios were paralleled by altered collagen quality criteria. The contrary was found in the case of the archaeological specimens. This implies that the commonly used collagen quality criteria may be insufficient and do not guarantee that stable isotopic values of the gelatine extracts will still represent the original biological signal.     

Repeat silica extraction: a simple technique for the removal of PCR inhibitors from DNA extracts

Polymerase chain reaction (PCR) inhibitors are often co-extracted with ancient DNA (aDNA) and when present make the analysis of aDNA difficult, if not impossible. In this study we review previous research on PCR inhibitors and techniques that address their co-extraction with DNA from sub-optimal samples. Additionally, we introduce a simple extraction technique, “repeat silica extraction,” that effectively removed PCR inhibitors from extracts of 7000–8000-year-old human skeletal remains from the Windover archaeological site in Florida and 700–2000-year-old human coprolites excavated from Fish Slough Cave in southern California. A series of tests on these same samples demonstrates that N-phenacylthiazolium bromide is largely ineffective, despite previously reported success using this compound as part of the DNA extraction process. We also describe a method for demonstrating the presence as well as successful removal of PCR inhibitors by use of a “positive aDNA control,” a test necessary to conclude that negative PCR amplification is the result of the absence of preserved DNA.     

Diet in the Iron Age cemetery population at Wetwang Slack,East Yorkshire,UK: carbon and nitrogen stable isotope evidence

This paper reports δ¹³C and δ¹⁵N values for human and animal bone collagen from the middle Iron Age site at Wetwang Slack, East Yorkshire, UK. The data indicate a human diet which was high in animal protein, with no evidence for any significant marine food input. No differences were found between high-status vehicle (or ‘chariot’) burials and the rest of the population and no other status differentiations are visible according to burial rite, age or sex groupings, although the data obtained for the older males display an unusual trend. No dietary variation is seen between two site phases and no evidence for an early immigrant group is present. The range of isotope values for the adult human group as a whole is small, indicating that the diet is likely to have been consistent over time and across the population, although two individuals stand out as unusual amongst the 62 analysed.     

Mobility in Central European Late Eneolithic and Early Bronze Age: tibial cross-sectional geometry

An absence of settlement features during the Central European Corded Ware period (Late Eneolithic, 2900–2300 BC) has been interpreted as a reflection of mobile pastoral subsistence. Recent analyses of the Late Eneolithic archeological context reveal that the Late Eneolithic exhibit evidence of sedentary agricultural activities similar to the Early Bronze Age. Since the archeological analyses are not clear cut, we tested mobility pattern differences between the Late Eneolithic and Early Bronze Age using biomechanical analysis of the tibial midshaft cross-sections. The total sample of the 130 tibiae representing five archaeological cultures was used. The results of the tibial midshaft geometry do not support the hypothesis about different mobility in the Late Eneolithic and Early Bronze Age. This conclusion is supported by nonsignificant differences between the Corded Ware females and the Early Bronze Age females. Higher absolute values for the Corded Ware males should be explained either by stochastic variation or by differing amounts of physical demands despite a generally similar pattern of subsistence of the Late Eneolithic and Early Bronze Age. One of the Early Bronze Age samples, the Wieselburger group, is an exception because the individuals show both reduced overall size and bending resistance of the tibial parameters not only in comparison with the Late Eneolithic but also to the rest of the Early Bronze Age. The results suggest that the behavioral processes which affected the tibial midshaft biology operated during the Late Eneolithic and Early Bronze Age as a mosaic across time and between/within cultures.     

Dietary patterns during the late prehistoric/historic period in Cikobia island (Fiji): insights from stable isotopes and dental pathologies

The dietary patterns of eight adults and one child interred in the Korotuku burial mound (Cikobia, northern Fiji), dated from the late prehistoric/historic period (around AD 1850), have been investigated using carbon and nitrogen stable isotope signatures and dental and oral pathologies. Bone collagen isotopic compositions showed that (1) males and females had similar diets in terms of terrestrial C₃ resources and marine fish components and proportions; (2) the proportion of marine fish comprised around 25% of the protein fraction of the diet, and the proportion of vegetal food was high; and (3) one canid that was also studied had a different diet, probably rich in shellfish. Oral and dental examinations of the humans evidenced (1) a low level of dental macrowear, (2) a high rate of carious lesions—interproximal and cervical—of about 15%, and (3) a low amount of abscessing, as well as the presence of alveolar bone recession and calculus in most of the adults. This evidence suggests a diet relying mainly on vegetal food with very limited shellfish consumption. When viewed in the light of ethnohistoric information, this pattern suggests a particular sociocultural behaviour, including food selection, since the analyses of the canid remains indicate that more diversified food resources were available than those consumed by the humans. Overall observations suggest that the deceased in the Korotuku burial mound might be members of an elite living on the island, thus pointing to possible social stratification in the late prehistoric/historic Cikobia community.     

A brackish water aquatic foodweb: trophic levels and salinity gradients in the Schlei fjord, Northern Germany, in Viking and medieval times

This study serves for the definition of baseline isotopic signatures of vertebrates living in the Schlei fjord, a brackish water inlet in the north German plain, where the salinity decreases from its mouth at the Baltic Sea towards the inland locations. The Viking trade centre Haithabu and its immediate successor, the town of Schleswig, are located at opposite banks of the Schlei and constitute a settlement continuum from the 9th until the 13th century. This development not only includes a relocation of the settlement site, but also a change in economy and social structure, and witnesses the metamorphosis from an international trading locality to the rise and decline of a prototypical medieval town with town charter. In this paper, stable isotope ratios of bone collagen and bone structural carbonate of 141 vertebrate bone finds mainly of fish, birds, and sea mammals from both sites are reported to provide the baseline food web in this brackish water environment, which is the prerequisite for our ongoing studies aiming at the reconstruction of human subsistence strategies as well as the geographic origin of humans, animals, food stuffs, and raw materials in order to complete the historical picture of emerging medieval towns in a complex palaeoecosystem. In particular, we are able to show how a combination of stable isotopes from bone collagen and carbonate is capable of defining the salinity gradients in the aquatic environment.     

Prehistoric Fijian diet and subsistence: integration of faunal, ethnographic, and stable isotopic evidence from the Lau Island Group

We explore subsistence practices and dietary change on islands in the central Lau Group of Fiji with zooarchaeological methods and stable isotopic analysis of human and animal skeletal material interpreted through an ethnoarchaeological lens. Our dataset combines detailed identifications of fauna, especially fishes, with stable isotopic (δ¹³C, δ¹⁵N) values of human and animal bone collagen and apatite carbonate spanning approximately three thousand years of human occupation on three study islands. Additionally, over fifty inshore and offshore contemporary fishing expeditions were observed on all study islands over a six-year period. We integrate these separate lines of evidence to form robust interpretations of Lauan subsistence patterns over a broad temporal scale. We add to the existing literature on stable isotopic analysis of archaeological bone from Remote Oceania and compare all of these groups through time. Our results indicate that Lauans differentially relied on nearshore reef resources rather than pelagic fishes, and terrestrial endemic species may have served as a portion of the diet during the early prehistoric period. Root crops (e.g., taro, yam) provided the majority of calories to the diet; however, sea grapes likely contributed to the early diet. Our isotopic results differ from previous studies of Fijian diet and that of Remote Oceania at a time of probable marine ecosystem shifts (AD 1300) illustrating diet breadth and variability in subsistence strategies potentially due to climatically influenced resource depletion.