Pre-screening techniques for identification of samples suitable for radiocarbon dating of poorly preserved bones

Under certain environmental conditions, post-depositional diagenetic loss of bone collagen can severely reduce the number of bones from a particular archaeological site that are suitable for stable isotopic analysis or radiocarbon dating. This study examined nearly 300 bones from 12 archaeological sites across southern England known to yield poor or variable preservation to try to identify one, or more, pre-screening technique(s) that would indicate suitable collagen preservation for radiocarbon dating. The most reliable method was shown to be the percent nitrogen (%N) of whole bone powder, which has an 84% chance of successfully predicting whether or not a bone will yield sufficient (i.e. >1% weight) collagen for dating.     

Dating of modified femora of extinct dwarf Hippopotamus from Southern Madagascar: Implications for constraining human colonization and vertebrate extinction events

Two significant events in the late Holocene history of Madagascar were (a) the arrival of people, and (b) the loss of nearly two dozen species of land vertebrates in the socalled “subfossil extinctions”. The consensus is that the faunal losses occurred shortly subsequent to human arrival, but the timing of these events is poorly constrained. The minimum age for initial human presence on the island may now be set at approximately 2000 bp, on the basis of AMS ¹⁴C dates for human-modified femora of extinct dwarf hippos from SW Madagascar. Assuming that this date also marks the beginning of deleterious human interactions with the subfossil fauna, and assuming that this fauna became completely extinct by 900 bp, the width of the anthropogenic “extinction window” may have been as long as c. 1000 a. This estimate, nearly twice the length of previous ones, is close to the unadjusted minimum for the duration of the terminal Pleistocene extinction event in the Americas. Whether or not this length of time comports with theoretical expectations of a “blitzkrieg” pattern of losses is uncertain, but greater refinement in dating the end of the subfossil extinctions is unlikely to produce radically shorter estimates of duration.     

Injuries to the skeleton due to prolonged activity in hand-to-hand combat

Two complete skeletons from the Hamman-Todd collection of the Cleveland Museum of Natural History (CMNH) show a suite of pathological lesions that suggest the individuals had been involved in boxing or other hand-to-hand combat. These lesions were studied and compared with medical and autopsy records. The aims of the research were to estimate the accumulated damage to the bones over time, to characterize the different types of the damage, and to establish criteria for hand-to-hand combat or violence for archaeological material. Our inspections showed that besides the muscle markings developed and the numerous healed fractures that are expected when someone is involved in such activities, other types of lesion are present that are helpful for a proper differential diagnosis. These are: degenerative changes at the lesser tuberosity of the humerus; focal necrotic changes/bone growth on the trochlea of the humerus; necrotic changes on the distal head of the ulna; bony patches on upper limb bones only; secondary centres of ossification failing to fuse (mainly in vertebrae and acromion); a huge conoid tubercle on the clavicle; bony spurs on the distal articular head of the metacarpals; necrotic changes on the femoral head next to the fovea and on the roof of the acetabulum; and a developed bony ridge for the attachment of the iliotrochanteric ligament. Finally, we propose a set of criteria that will help to identify people in archaeological material who were involved in hand-to-hand combat.     

Examining histology to identify burned bone

Early research suggests that examination of the microscopic internal structure is a valid method for distinguishing burned from unburned bone in the archaeological record. This study compares burned and unburned modern bones with archaeological bones from Sibudu Cave (ca. 60,000 years ago) and Cave of Hearths (over 200,000 years ago) to more fully describe the heat-induced histological changes to bone. We also explore the effects of diagenesis on those changes to determine if histological evidence of burning preserves through fossilisation and diagenetic processes and can be successfully used to identify ancient burning. Application of this technique can help in addressing various issues, including the origins of controlled use of fire and understanding animal butchery and disposal.     

Tending and drive hunting: A density-mediated attrition model can explain age profiles of white-tailed deer at Iroquoian village sites

In a 1993 paper, Noble and Crerar suggested, based on the age and sex distribution of white-tailed deer (Odocoileus virginianus) longbones from four Attawandaron Iroquoian sites around the western end of Lake Ontario, that the contact-period Attawandaron were managing local deer populations, in response to the aboriginal trade in hides. Having observed similar patterns of differential preservation of elements and epiphyseal fusion at nearby Iroquoian sites that are not ascribed to the Attawandaron, and which pre-date the hypothesised trading period, we argue that these patterns may instead relate to bone density and/or vulnerability to taphonomic effects of late-fusing epiphyses. We further support our argument through age profiles based on dental eruption and wear.     

Investigating the potential of micro-focus computed tomography in the study of ancient bone tool function: results from actualistic experiments

Many experiments have sought to recreate the types of damage that would be expected in ancient stone and bone weapon tips. This damage is usually presented as visible fractures or microscopic surface modification. Fatigue tests conducted on bovine bones, however, show the development of internal micro-cracks that result from stress, prior to actual breakage. In this paper I present the results of an experimental investigation of bone points subjected to a variety of activities. I assess the presence of microdamage using micro-focus computed tomography. The results show that two patterns of micro-cracks develop in bone and are best viewed in longitudinal section. Micro-cracks are a cumulative feature dependent on the amount of load applied and the duration of activity. When subjected to high enough loading rates, micro-cracks will merge together to eventually form a fracture. Although further tests are needed to confirm the exact point at which these fatigue fractures begin to form, micro-focus computed tomography has the potential to reveal whether an individual bone point underwent multiple or prolonged impacts and thus to elucidate the probable function/s of ancient pointed bone tools where no visible damage is apparent. Micro-focus computed tomography is a non-destructive and non-invasive procedure and therefore safe to use on archaeological artefacts.     

Late Pleistocene Crocuta crocuta spelaea (Goldfuss, 1823) clans as prezewalski horse hunters and woolly rhinoceros scavengers at the open air commuting den and contemporary Neanderthal camp site Westeregeln (central Germany)

Late Pleistocene Ice Age Crocuta crocuta spelaea ( Goldfuss, 1823) hyenas from the open-air gypsum karst site Westeregeln (Saxony-Anhalt, central Germany) is dated into the early to middle Late Pleistocene. Hyena clans apparently used the karst for food storage and as “commuting den”, where typical high amounts (15% of the NISP) of hyena remains appear, also faecal pellets in concentrations for den marking purposes. Additionally small carnivores Meles, Vulpes and Mustela appear to have used some cavities as dens. Several hundreds of lowland “mammoth steppe fauna” bones (NISP = 572) must have been accumulated primarily by hyenas, and not by Neanderthals at the contemporary hyena/human camp site. Abundant caballoid horse remains of “E. germanicus Nehring, (1884)” are revised by the holotype and original material to the small E. c. przewalskii horse. Woolly rhinoceros Coelodonta antiquitatis remains are also abundant, and were left in several cases with typical hyena scavenging damages. Rangifer tarandus (11%) is mainly represented by numerous fragments of shed female antlers that were apparently gathered by humans, and antler bases from male animals that were collected and chewed in few cases (only large male antlers) by hyenas. The large quantities of small reindeer antlers must have been the result of collection by humans; their stratigraphic context is unclear but such large quantities most probably resulted from schamanic activities. The hyena site overlaps with a Middle Palaeolithic Neanderthal camp, as well as possibly with a later human Magdalénian site.     

Re-investigating fish consumption in Greek antiquity: results from δC and δN analysis from fish bone collagen

One of the frequently encountered issues in ancient Greek dietary reconstructions through isotope analyses has been the apparent unimportance of fish protein in human diets. The significance of this observation is amplified by the abundant ichthyofaunal remains, iconographic evidence and literary information on fish and fishing, pertaining to almost all sites and time periods of Greek antiquity. In this project, we measured for the first time isotopes from a large number of fish bones from Greek sites dating from the Mesolithic to the Classical times, aiming to investigate whether this absence is an artefact of the methodology or whether it reflects a reality of restricted fish consumption. Results show that regional trends are stronger that temporal ones in fish isotope values. The range of values overlaps with terrestrial resources, making it difficult or impossible to reject fish consumption based on isotope data alone. This variability proposes a reconsideration of the amount of fish in ancient Greek diets specifically for each site and amplifies the importance of interdisciplinary studies, especially for regions with variable ecological resources.     

Carbon and nitrogen isotope signals in eel bone collagen from Mesolithic and Neolithic sites in northern Europe

Carbon and nitrogen isotope analysis was undertaken on collagen extracted from eel bone from six Mesolithic and Neolithic sites in northern Europe. The results were compared with data obtained on other archaeological fish remains and modern eel caught in fresh and brackish water and from the sea. The possibility of discriminating between freshwater, brackish and marine signals in archaeological eel bone is evaluated and the implications for archaeology discussed. Our data suggest that eel found at coastal Mesolithic and Neolithic sites have carbon isotope signals consistent with a marine origin with no evidence of freshwater residency. The sample of eel bone from one inland site is small but indicates carbon isotope values more consistent with freshwater residency or at least values intermediate between freshwater and marine carbon pools.     

Changes in human bones boiled in seawater

The differences between boiled or unboiled bones are not often studied. However, they are crucial to understand postmortem rituals and to establish defleshing procedures and mortuary practices. In this work, human bones boiled in sea or fresh water are characterized. The bone composition, as well as the compounds present in the resulting materials, shows that salt alters the boiling process mechanism. Hence, from structural and morphological criteria, it is possible to distinguish if a bone has been boiled in salt or fresh water. In both sets of samples, the smoothness of the bone surface depends on boiling time, but only in bones boiled in seawater, filaments are observed apparently pouring out of the pores.Those differences which are mainly morphological (smoothness of the surface) are explained in terms of a collagen diffusional mechanism favored by sodium and chloride ions. For a boiling time of 6 h, the surface is covered by a thick layer or crusts of degraded collagen. Experiments with seawater may be used as model experiments to simulate taphonomical alterations in bones exposed to salt water.