Aging mountain gazelle (Gazella gazella): refining methods of tooth eruption and wear and bone fusion

This paper refines current methods for aging mountain gazelle (Gazella gazella), one of the most common species hunted by prehistoric humans in the southern Levant. Most importantly, we present a new tooth wear scheme for aging the lower deciduous fourth premolar (dP₄), the lower permanent molars (M₁, M₂, M₃) and the lower fourth premolar (P₄) of the gazelle. Broader wear stages for complete mandibles and individual teeth and bone fusion stages are also provided. Finally, a comparison of the tooth wear and bone fusion data indicates that the two methods can be linked at the important transition when a gazelle reaches adulthood (ca. 18 months of age). The completion of the fusion of the gazelle skeleton corresponds directly to the replacement of the dP₄ by the P₄ and the beginning of wear of the M₃. The coincidence of these aging indicators allows for direct comparison of gazelle bone fusion and tooth wear data.     

A new experimental study on percussion marks and notches and their bearing on the interpretation of hammerstone-broken faunal assemblages

Preliminary experiments on bone breakage have shown the potential utility of quantifying some of its diagnostic features (percussion marks, percussion notches) for taphonomic inferences about human and carnivore involvement in bone breakage in faunal assemblages. The present study increases the range of experiments undertaken to understand the identification of hammerstone percussion (dynamic loading) and its differences from carnivore bone breakage through tooth pressure (static loading). This study contributes to a better understanding of frequencies of percussion marks, uncovers and quantifies those percussion marks that lack key diagnostic features to be identifiable and that could be mistaken with carnivore tooth marks, quantifies notch types, shows different size ranges for notches on bones from small and large fauna than previously reported, quantifies the proportion of notches bearing percussion marks, and introduces new size data for percussion (impact) flakes and percussion marks. Furthermore, all these variables have been applied to a dual experimental set: one experiment using non-modified hammerstones and another based on the use of modified hammerstones. Results vary considerably according to hammerstone type. Some of these taphonomic variables increase the range of equifinality when identifying marks and notches created by different human and non-human agents. This calls for further caution when using isolated variables and features rather than a holistic approach to make taphonomic inferences.     

Aspartic acid racemization variability in ancient human remains: implications in the prediction of ancient DNA recovery

The extent of racemization of aspartic acid (Asp) – expressed as d/l ratio – has been used as a marker of biomolecular degradation in ancient remains. However, Asp racemization rate is highly variable, and depends on biochemical and geochemical factors. In this paper we aim to determine to which extent the fraction analyzed and the kind of sample used may influence the d/l Asp ratios. Other factors, such as burial site and sample preservation conditions, are also considered.     

Lead concentrations in bones and soil

Lead concentrations were measured in 41 rib samples from the Romano-British cemetery at Poundbury and in two sets of soil samples from the graves yielding up the bones. The levels of lead in the soil were in no way exceptional (means for the two series were 16·7 and 24·8 μg/g dry weight) and there was no correlation between soil lead and bone lead concentrations.Since bone lead concentrations vary independently of those in the soil, it is unlikely that the bones are contaminated by lead in the soil, at least under the conditions prevailing at this particular site.     

Ban Chiang and Northeast Thailand; the palaeoenvironment and economy

Beginning with a detailed presentation of the faunal spectra from Ban Chiang and related sites in Northeast Thailand, this essay reconstructs the palaeoenvironment during the period 3500 BC to the end of the prehistoric period. The evidence from the freshwater molluscs found in prehistoric layers suggests that the first occupants of Ban Chiang encountered a habitat with permanent lakes and clear, slow moving streams. Since the lakes contracted in the dry season, there were ideal conditions for practising wet swidden agriculture. From c. 1600 BC, the presence of water buffalo and associated changes in the faunal spectrum suggests the inception of wet rice cultivation. Such agricultural intensification, it is held, follows population pressure and accounts for subsequent settlement in the more arid plains of Thailand.     

Implications of heat-induced changes in bone on the interpretation of funerary behaviour and practice

Bones submitted to heat experience structural and chromatic modifications. In particular, heat-induced bone warping and thumbnail fractures have been linked to the burning of fleshed and green bones – where the soft tissues have been removed from the bones soon after death – in contrast to dry bones. Those have been suggested as indicators of the state of the individual before being burned thus allowing inferences about the funerary behaviour of archaeological populations. A large sample of 61 skeletons submitted to cremation has been examined for the presence of both of these heat-induced features. Although uncommon, bone warping and thumbnail fractures were present in some of the skeletons demonstrating that its presence is not restricted to the burning of non-dried bones as generally believed. Rather than being an indicator of the presence of bones with soft tissues, bone warping seems to be more of an indicator of the preservation of collagen–apatite links which can be maintained on dry bones with low collagen deterioration. In addition, our results also do not confirm thumbnail fractures as an exclusive sign of the burning of bones with soft tissues. As a result, these heat-induced changes should be used with caution when trying to infer about the pre-burning state of an individual.     

A calf for all seasons? The potential of stable isotope analysis to investigate prehistoric husbandry practices

The Early Bronze Age barrows at Irthlingborough and Gayhurst in central England are notable for the large number of cattle (Bos taurus) remains associated with their human Beaker burials. Previous work using strontium isotope analysis has indicated that most of the cattle analysed, and one aurochs (Bos primigenius), were of local origin [Towers, J., Montgomery, J., Evans, J., Jay, M., Parker Pearson, M., 2010. An investigation of the origins of cattle and aurochs deposited in the Early Bronze Age barrows at Gayhurst and Irthlingborough. Journal of Archaeological Science 37, 508–515.]. In this study, stable isotope analysis of enamel and bone was carried out to investigate whether the mature cattle had experienced similar husbandry practices, climate and environment. Bulk carbon, nitrogen and sulphur isotope analysis of collagen suggested most were consuming similar sources of plant protein from environments probably local to the sites and this was supported by high resolution intra-enamel carbon isotope profiles. Oxygen isotope profiles indicated the aurochs and most of the cattle experienced similar climatic regimes: the only exception being an animal with a non-local strontium isotope ratio. However, a comparison of seasonality profiles of the local animals using estimated tooth formation times showed that there was no consistency in season of birth: the animals appeared to have been born throughout the year. Cattle can breed throughout the year but it requires considerable human effort and intervention to successfully overwinter young stock; it is therefore unlikely to have been carried out without good reason and benefit if winters were harsh. One reason is to ensure a continuous supply of milk. Measuring oxygen isotope profiles to identify year-round calving may thus be a potential indicator of dairying economies.     

Micro-sampling of human bones for mobility studies: diagenetic impacts and potentials for elemental and isotopic research

The nature of long bone formation and the pathways of interaction between bone samples and the burial environment suggest that portions of the bones disconnected from the arterial system are resistant to diagenetic alteration. Preliminary work on femurs from Early Bronze Age hunter-gatherers in Cis-Baikal, Siberia shows that the nature and progression of chemical changes in the bone matrix due to microbial attack can be analyzed using laser ablation inductively coupled plasma mass spectrometry. Intra-osteon variability in elemental concentrations and strontium isotope ratios (⁸⁷Sr/⁸⁶Sr) indicate the presence of unaltered portions of bone within diagenetically modified bone and suggest that useful data remain accessible. These biogenic signals can potentially be useful for mobility research in broad terms and the smaller timescales within an individual's lifetime (months, years), accessible therein. Laser ablation micro-sampling of femur specimens showed that intra-osteon elemental composition of Ba, Re, and Cs varied within and was correlated between multiple osteons of a single bone. Portions of chemically unaffected bone were identified within, and effectively discriminated from diagenetically altered bone tissue. Areas showing visual alterations and erratic or uncorrelated Ca and Sr elemental results also had anomalous Sr isotope ratios, suggesting diagenetic alteration in those places. Compositional and isotopic analysis of intact portions of bone supports the hypothesis that hunter-gatherer groups in Cis-Baikal made numerous major movements during their lives. Microscopic analysis of long bones clarifies aspects of biodeterioration and correlations between trace elemental results and diagenetic alteration. Micro-sampling of intact portions of bone expands the scope of available materials for research on mobility and other aspects of human past behavior.     

Biofilm growth in human skeletal material from ancient Mesopotamia

Investigators have long recognised the effects of microbial activity on archaeological bone. These investigators, however, have focused on single or groups of microbes rather than on complex microbial aggregates such as biofilms, a focus that has affected our understanding of archaeological bone biodeterioration. In this paper, we report on the investigation of a biofilm in archaeological human bone from the site of Tell Leilan, Syria (2900–1900 BCE). Scanning electron microscopy indicated that the biofilm is characterised by single cells and microcolonies of bacteria and fungi, as well as calcite crystals that were all embedded within extracellular polymeric substances. Using culture techniques and DNA sequencing, we isolated and identified several microbes from the biofilm including Amycolatopsis sp., Penicillium chrysogenum, Aspergillus sp., Chaetomium sp., and Cladosporium sp. Having characterised the Leilan biofilm, we are now closer to understanding the complex process of bone biodeterioration in archaeological bone collections.     

Human–environment interactions in medieval Poland: a perspective from the analysis of faunal stable isotope ratios

Stable isotope analyses of faunal remains provide valuable information about human–environment interactions in the past, including insights into past animal husbandry and land management strategies. Here, we report stable carbon (δ¹³C) and nitrogen (δ¹⁵N) isotope values of collagen and carbonate from archaeological fauna from Ka?dus, a medieval settlement in North-Central Poland, to better understand human–environment interactions during a period of increasing urbanism and marketization. Wild and domestic animals can be separated on the basis of their isotopic values. The mean δ¹⁵N value for 12 domesticated animals is 7.6 ± 1.2‰ and for 5 wild animals is 4.3 ± 0.5‰ (p = 0.002). The mean collagen δ¹³C value for domesticated animals is −20.6 ± 1.1‰ and for wild animals is −22.0 ± 0.5‰ (p = 0.004). The mean carbonate δ¹³C value for domesticated animals is −13.14 ± 1.3‰ and for wild animals is −14.14 ± 0.9‰ (p = 0.034). The “canopy effect” and anthropogenic effects that alter stable isotope ratios of plants (manuring, swidden agriculture and ploughing) are discussed in relation to these differences. Fish are isotopically variable, which suggests broad-spectrum fishing strategies and/or trade, and increases our awareness of the difficulties in interpreting human paleodiet when freshwater fish were on the menu.