Earthenware production and distribution in the prehispanic Philippine polity of Tanjay: results from laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS)

This paper presents the results of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) analysis of low-fired earthenware from the prehispanic trading polity of Tanjay (c. A.D. 500–1600) in the central Philippines. The goal of this project was to determine whether or not the compositional analysis of ceramics could be used to examine the organization of pottery production and exchange relationships in the Bais-Tanjay region of Negros Island. Results indicate that at least five distinct groups of ceramics can be differentiated based on elemental compositions and that pottery production was both a part-time, dispersed endeavor and a full-time, likely centralized, craft. In addition, a number of compositional outliers in the ceramic dataset suggest that interregional trade took place, with pottery made outside the Bais-Tanjay region being imported, especially by upland swidden farmers.     

Fish ’n chips: ZooMS peptide mass fingerprinting in a 96 well plate format to identify fish bone fragments

Fish are a large, highly diverse, and anthropologically important group of vertebrates. However, fish bones are underrepresented in the archaeological literature because they are less stable than those of other taxa and identification of bone to species is often difficult or impossible. We explore a new identification system, ZooMS (Zooarchaeology by Mass Spectrometry), which is based upon protein barcoding. As proteins can be cleaved enzymatically and analyzed by mass spectrometry in a repeatable way, protein barcoding is used widely in microbiological contexts for quick and inexpensive protein identification; mass spectra reflect the differences in protein sequence and can therefore be reproducibly linked to a particular protein or protein fragment. ZooMS uses peptide fingerprinting of bone collagen as a method for rapid identification of archaeological bone. This has involved the identification of masses related to peptides of known sequence. For mammals, sufficient sequence information is available for this approach but for groups, such the teleost fish, species are highly diverse and there are few available collagen sequences. Here we report a preliminary investigation into the identification of fish species by peptide mass fingerprinting that does not require sequence information. Collagen mass spectra are used to identify eight species of bony fishes (Osteichthyes) from four orders: Clupeiformes, Salmoniformes, Gadiformes, and Perciformes. The method is applied to both modern and archaeological fish remains and offers the capacity to identify traditionally unidentifiable fish fragments, thus increasing the Number of Identified Specimens (NISP) and providing invaluable information in specialized contexts.     

Microstructural,chemical and isotopic evidence for the origin of late neolithic leather recovered from an ice field in the Swiss Alps

Archaeological leather samples recovered from the ice field at the Schnidejoch Pass (altitude 2756 m amsl) in the western Swiss Alps were studied using optical, chemical molecular and isotopic (δ¹³C and δ¹⁵N of the bulk leather, and compound-specific δ¹³C analyses of the organic-solvent extracted fatty acids) methods to obtain insight into the origin of the leather and ancient tanning procedures. For comparison, leathers from modern native animals in alpine environment (red deer, goat, sheep, chamois, and calf/cow) were analyzed using the same approach. Optical and electron microscopically comparisons of Schnidejoch and modern leathers showed that the gross structure (pattern of collagen fibrils and intra-fibrils material) of archaeological leather had survived essentially intact for five millennia. The SEM studies of the hairs from the most important archaeological find, a Neolithic leather legging, show a wave structure of the hair cuticle, which is a diagnostic feature for goatskins. The variations of the bulk δ¹³C and δ¹⁵N values, and δ¹³C values of the main fatty acids are within the range expected for pre-industrial temperate C₃ environment. The archaeological leather samples contain a mixture of indigenous (from the animal) and exogenous plant/animal lipids. An important amount of waxy n-alkanes, n-alkan-1-ols and phytosterols (β-sitosterol, sitostanol) in all samples, and abundant biomarker of conifers (nonacosan-10-ol) in the legging leathers clearly indicate that the Neolithic people were active in a subalpine coniferous forest, and that they used an aqueous extract of diverse plant material for tanning leather.     

Indications of embalming in Roman Greece by physical,chemical and histological analysis

The partially mummified remains of a high-status female (ca. 1700 BP, Thessaloniki, Greece) were found inside a Roman-type marble sarcophagus containing a lead coffin. The individual was positioned on a wooden pallet, wrapped in bandages, and covered with a gold-embroidered purple silk cloth. Besides the clothes, remnants of soft tissue as well as the individual's original hair style and eyebrows were exceptionally well preserved. In addition to the macroscopic examination, microscopic and biochemical analyses were undertaken. Scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) analysis, and gas chromatography/mass spectrometry (GC/MS) were applied to examine the tissue preservation and probable mechanisms of mummification. The presence of chemical components, such as sesquiterpenes, triterpenoids, and diterpenoids, originating from coniferous and pistacia resins, myrrh, and other spices, verify ancient information on preparation methods of the dead in Greek and Roman times. These chemical components are thought to have played a prominent role in the mummification mechanism in this particular case. The potential effect of the lead coffin in the mummification process was also examined. Energy-dispersive X-ray analysis failed to detect lead penetration into the tissues, suggesting that the coffin played a limited role in the preservation of soft tissue.     

Colour in Minoan faience

The microstructures and chemical compositions of some 15 faience objects from Crete spanning the period from Middle Minoan IIIA through to Late Minoan IA are determined using analytical scanning electron microscopy. The Minoan faience is compared with replicate faience beads produced in the laboratory using various combinations of manganese, copper and iron as colorants. The alkali contents of the replicate beads are varied so that the colorants are present both as ions in a glass phase and as particulate oxides. These data are then used to try to infer the original colour of the Minoan faience, the great majority of which has suffered severe weathering during burial. The results suggest that instead of the present day “hallmark greys and browns” and “subtle greens and blues”, Minoan faience originally exhibited a wide range of colours, including bright turquoise blue, purple and violet, and pale yellow-green and greenish turquoise.     

AMS C dating of early human occupation of southern South America

The time of appearance of a persistent and demographically-viable hunter-gatherer population in late Pleistocene southern South America must be determined by evaluating evidence from as large as possible a sample of candidate archaeological sites in the region. We co-ordinated the AMS dating of multiple bone and charcoal samples from previously-excavated strata at the following sites: Arroyo Seco 2, Paso Otero 5, Piedra Museo, and Cueva Tres Tetas (all in Argentina), and Cueva del Lago Sofia 1 and Tres Arroyos (both in Chile). With one possible exception, we did not obtain new results to confirm earlier observations of pre-Clovis-age cultural activity at any of the sites considered in this study. The possible exception, Arroyo Seco 2, is considered in detail elsewhere [Politis G., Gutierrez M.A., Scabuzzo, C. (Eds), in press. Estado actual de las Investigaciones en el sitio 2 de Arroyo Seco (región pampeana, Argentina). Serie Monográfica INCUAPA 5. Olavarría]. However, our results for the samples which were the most preferred indicators of cultural events (hearth charcoal and cut-marked bone) confirm that people were in the southern cone of South America at or soon after 11,000 BP (13,000 cal BP). Considered alongside recent age estimates for the Clovis culture in North America, these results imply the contemporaneous emergence of a consistent and archaeologically-robust human occupation signal at widely-separated locations across the Western Hemisphere. Such findings suggest that Palaeoindian demic expansion may have involved more than one terminal Pleistocene dispersal episode.     

Re-examining the Egyptian colonial encounter in Nubia through a compositional,mineralogical, and textural comparison of ceramics

Textural, mineralogical, and X-Ray fluorescence (XRF) analyses are used as a cost effective method to distinguish for the first time ancient Egyptian and Nubian-style ceramics found in Nubia. Textural and mineralogical data suggest that Nubian-style sherds are mixtures of sand, silt, and clay sediment that is generally finer grained and poorer in quartz than is the sediment mixture used to produce Egyptian-style pottery. Chemical data also establish a significant difference in the amount of chemical variation found within each style population, but nonetheless supports a considerable overlap in the possible provenance of both styles. Observations and data obtained during this study indicate that standardized, Egyptian-style manufacturing was introduced into Nubia under colonialism, without substantially altering the diversity of native methods of production.