Molecular and morphological analyses of avian eggshell excavated from a late thirteenth century earth oven

Using ancient DNA (aDNA) extracted from eggshell of the extinct moa (Aves: Dinornithiformes) we determined the species composition and number of eggs found in a late thirteenth century earth oven feature at Wairau Bar (South Island, New Zealand) – one of New Zealand’s most significant archaeological sites. Mitochondrial and nuclear DNA signatures confirmed this oven feature contained fragments of at least 31 moa eggs, representing three moa genera: Emeus; Euryapteryx; Dinornis. We demonstrate through the genetic identification of 127 moa eggshell fragments that thickness is an unreliable character for species assignment. We also present a protocol for assessing the preservation likelihood of DNA in burnt eggshell. This is useful because eggshell fragments found in archaeological contexts have often been thermally modified, and heat significantly increases DNA fragmentation. Eggshell is widely used in radiocarbon dating and stable isotope research, this study showcases how aDNA can also add to our knowledge of eggshell in both archaeological and palaeoecological contexts.     

Species identification of archaeological dung remains: A critical review of potential methods

Abstract

Dung, macroscopically recognisable as such or not, can more commonly be found in archaeological contexts than is perhaps realised. Up to now, identification of dung to the species which produced it is usually either tenuous, or is not possible. However, species identification can be very informative and is necessary before any further studies can be conducted on the dung, for example on health and hygiene in the past and palaeoecology. This study presents a review of potential methods by which species identifications of archaeological dung can be undertaken. Criteria for identification can be divided into three broad categories: morphometric features of the dung; the content of dung and contextual evidence. Overall, the chances of a precise identification are high; however, a combination of different criteria and techniques will often be necessary to establish a secure identification. Moreover, preservation issues may exclude the application of some criteria while several criteria require more research and the expansion of reference collections of recent material. The overall aim is to move towards standardised methods for species identification of archaeological dung.     

建水指林寺大殿木构件材种鉴定及配置研究

木材树种的鉴定是古木结构建筑维修和保护的基础性工作。为配合建水县指林寺大殿修缮保护的需要,为修缮树种选用提供依据,了解木文化的特点。本研究对建水指林寺大殿古建筑主要木构件进行了树种鉴定及对材种配置调查分析。采用切片、显微镜拍照、宏观和微观特征分析等方法,对700个试样进行了树种鉴定。结果表明建水指林寺大殿的木构件共有4个树种,分别是硬木松(Pinus sp.)、白青冈(Cyclobalanopsis sp.)、格木(Erythrophleum sp.)和荷木(Schima sp.)。通过材种配置分析可得指林寺大殿木构件在选材方面达到很高水平,不仅反应了建造年代,而且体现修缮的特性。     

Trace Element Classification Diagrams of Pyroclastic Rocks from the Volcanic Districts of Central Italy: the Case Study of the Ancient Roman Ships of Pisa

Identification of the volcanic material (pozzolan) stored in a Roman ship of the fourth to third century bce , wrecked in the ancient harbour of Pisa (central Italy), was based on ratios of selected trace elements. The compositions of the major volcanic products erupted by the volcanoes of the Roman province, including the volcanic districts of Latium (Vulsini, Vico, Monti Sabatini and the Alban Hills) and Campania (Roccamonfina, the Phlegraean Fields, Ischia, Procida and Vesuvius) are compared with the pozzolan from the Pisa ship. Superposition of the Zr/Y, Nb/Y and Nb/Zr ratios of the pyroclastic material from the wrecked ship (computed from the published literature) allows correlation with the products of the Onano eruption from the Vulsini Volcanic District. The Vulsini rocks outcrop extensively in a sector drained by the hydrographic network of the Fiora River, which has been a well‐developed commercial water trade route since Etruscan times, with a river port and a sea port connected to the important town of Vulci, and, since 273 bce , part of the larger Roman harbour system of Cosa.     

Apollo and the Virgin: The Changing Meanings of a Sacred Site on Anafi

The ruins of an ancient Greek temple on a Cycladic island form part of an Orthodox Christian shrine. To local people and returning migrants, the site is that of their patron saint; its pre‐Christian past is irrelevant except in so far as it establishes their specific link with the ancient world, and thus with national claims of unbroken continuity between modern and ancient Greece. To early travellers and antiquarians, and to today’s archaeologists, it has been primarily the ancient site which is of interest, while tourists are more concerned in finding “an unspoilt island”. A historical survey of information about the ancient and the Christian site reveals ambiguities and confusions about both. These multiple meanings for locals and scholars are discussed.     

DNA analysis in charred grains of naked wheat from several archaeological sites in Spain

In the present work we attempt to recover endogenous ancient DNA from cereal grains preserved under different conditions: charred, partially charred and waterlogged. A total of 126 grains from naked wheat and 18 from barley from different sites on the Eastern Iberian Peninsula ranging from the beginning of agriculture in the region to the turn of the Common Era, were studied. Two different extraction protocols were used, a standard phenol–chloroform method and a silica-based DNA extraction procedure implemented for artificially charred seeds. Amplifications were directed to three markers: the large subunit of ribulose 1,5 biphosphate carboxylase (rbcL) and the microsatellite WCT12 in the chloroplast genome and the x and y subunits of the high molecular weight glutenin gene (Glu-1) in the nucleus. The first two were used to assess the preservation status of the samples, while with the third we tried to identify the wheat grains at species level. It was possible to obtain eleven positive amplifications in 8 partially charred seeds but only two amplifications of the Glu-1 gene from a single sample of the Early Bronze age were genome-specific. Different contamination sources were identified and reported. Cloning and alignment of sequenced clones showed a correspondence of the amplified fragment to modern wheat D genome haplotypes. This result suggests that the sample corresponds to hexaploid wheat (Triticum aestivum L.), thus being the first ancient DNA evidence to date for the cultivation of hexaploid wheat in the prehistoric agriculture of the Iberian Peninsula. Moreover, obtained results highlight contamination problems associated to the study of ancient archaeobotanical charred seeds suggest that the combination of a silica-based extraction method together with the amplification of specific targets is a good strategy for recovering endogenous ancient DNA from this kind of material.     

Radial route systems and agro-pastoral strategies in the Fertile Crescent: New discoveries from western Syria and southwestern Iran

Radial systems of spoke-like pathways, often termed “hollow ways,” are frequently found surrounding mounded tell sites in northern Mesopotamia and have been explained as the product of a particular set of land use practices involving dry-farming agriculture and intensive ovicaprid pastoralism. Yet while similar subsistence strategies were very common across the Near East throughout much of the Holocene, classic hollow ways have only been previously documented in a small region and articulate almost exclusively with sites of the third millennium BC. This paper presents newly discovered hollow ways in western Syria and southwestern Iran, made possible through analysis of an online database of declassified, Cold War-era CORONA satellite imagery. The association of these previously undocumented ancient roads with archaeological sites dating to the Iron Age, Roman/late Roman and early medieval periods, suggests that the land use practices which produced radial route systems may have been quite widespread. Taking into account the wide geographic and temporal distribution of hollow ways, analysis explores various aspects of the agro-pastoral systems that disparate communities may have shared. Results confirm some aspects existing models of hollow way formation, while offering some refinements in terms of the roles that settlement organization, agricultural land use and pastoral strategies play.     

Evaluation of carnivory in inland Jomon hunter–gatherers based on nitrogen isotopic compositions of individual amino acids in bone collagen

Archaeological studies including stable carbon and nitrogen isotope analyses of bone collagen from human remains have suggested their heavy dependence on terrestrial foods during the Jomon period in the inland central region in Japan. However, it is not easy to quantitatively evaluate the extent of carnivory for archaeological human remains based on the bulk collagen chemistry, because of variable ¹⁵N-enrichment factor along the trophic step and background isotopic variations in ecosystems. In order to overcome these problems and more precisely evaluate diets of prehistoric humans who strongly adapted to terrestrial environment, in this study we applied nitrogen isotope analysis of individual amino acids in bone collagen to two inland human populations in the Jomon period. Our results suggest that the two populations were predominantly dependent on the C₃-plant-based terrestrial ecosystem and consumed little aquatic resources. Furthermore, their mean trophic positions (2.7 for both cases) are closer to that of the fox (2.8–3.0) rather than those of pure herbivores (2.0–2.2), and show little change over time. These results are the first evidence that inland Jomon populations may have had more carnivorous diets than is traditionally considered.     

Lidar mapping and surface survey of the Izapa state on the tropical piedmont of Chiapas,Mexico

Recent lidar and pedestrian surface surveys have remapped the well-known Mesoamerican site of Izapa and the surrounding Soconusco piedmont. These data document: 1) occupation from the surrounding piedmont environment, 2) significantly larger estimates of the site's size during both the Formative and Classic periods as well as 3) new architectural features from the monumental site core. Methodological issues are outlined for combining high precision lidar mapping with ground truthing and pedestrian survey that focuses on surface collection of temporally diagnostic artifacts. Results are presented for 670 mounds documented in an area of 43.1 sq km in and around the ancient capital of Izapa.