Wild or domesticated: DNA analysis of ancient water buffalo remains from north China

Recent zooarchaeological studies on water buffalo (Bubalus sp.) remains from China and south Asia question the traditional view that water buffalo were first domesticated in Neolithic China over 7000 years ago. The results from several recent population genetic studies of modern domesticated buffalo (Bubalus bubalis) are not consistent with each other, placing the original center of buffalo's domestication in south Asia, southeast Asia, or China. This paper reports a study using an ancient DNA approach to analyze water buffalo remains from Neolithic sites in north China to investigate their affinities with modern domesticated water buffalo, and to shed light on the origin of modern domesticated water buffalo in China.A 169 bp fragment of D-loop mitochondrial DNA was successfully amplified and verified for 13 of 24 bone samples obtained from seven archaeological sites along the Wei River valley in Shaanxi Province, China. The bone samples which yielded positive DNA can be dated to 8000–3600 cal. BP. The phylogenetic analysis of the obtained DNA sequences along with modern water buffalo sequences indicated that the ancient water buffalos were not the direct ancestor of modern domesticated water buffalo. However, the phylogenetic analysis, along with BLAST searches of these ancient DNA sequences, did demonstrate their relatedness to water buffalo more so than to any other bovid species, confirming the existence of indigenous wild (but now extinct) water buffalo species (B. mephistopheles) in ancient China.The DNA analysis of these ancient remains failed to establish direct links between modern domesticated water buffalo (B. bubalis) and indigenous water buffalo (B. mephistopheles) from ancient China. If further DNA studies of more ancient remains from other regions of China confirm the observation of solely indigenous water buffalo species in ancient China, it would suggest modern water buffalo might not have been first domesticated in China.     

The Farasan Islands,Saudi Arabia: towards a chronology of settlement

An archaeological survey of Saudi Arabia's Farasan Islands in May 2010 recorded a broad range of sites that have not previously been documented. The survey concentrated on Greater Farasan and Segid islands, and comprised a rapid recording of sites shown to the authors by representatives of the Saudi Commission for Tourism and Antiquities. The sites were photographed, their positions logged, sketch drawings made of the principal features and surface pottery drawn and photographed. Detailed drawings were made of a stone anchor and a well with possible Ancient South Arabian carved decoration. The sites visited included settlements, wells, cemeteries and a cave. Several sites included the remains of buildings made of massive ashlar blocks, as well as others of rubble‐stone construction. Datable material at the sites points to several periods of occupation, from the early first millennium BC to early modern times. Some locations were characterised by long periods of settlement. Apart from the findings of this survey, most of the sites remain largely uninvestigated, and suggest significant potential for future research into settlement on the archipelago, as well as into past maritime activity and technology in the southern Red Sea region and beyond.     

安徽省图书馆藏《正德三年进士登科录》的修复

2013年初,为配合古籍数字化,安徽省图书馆开始修复馆藏《正德三年进士登科录》。通过分析破损现状、查找相关资料、咨询修复专家、制订修复方案、选配纸张、清洗书页、修补书页、书皮及托纸、修整书页、装订书册、残片修复与函套制作、完善修复记录,经精心修复,修复质量合格、效果满意。论文着重探讨了"古籍修复需要讲究依据"、灵活运用"修旧如旧"原则、保留修复资料等相关问题。此修复研究可为行业修复此类古籍提供相关借鉴。     

On the elimination of extraneous DNA in fossil human teeth with hypochlorite

Elimination of extraneous DNA in fossil specimens is of paramount importance for the successful isolation and analysis of authentic DNA; this is especially true when the specimens are of human origin. Bones and teeth are commonly decontaminated with bleach containing the powerful oxidising hypochlorite ion. The procedures involve either submersion in or wiping with the chlorine agent. Using the radioactive isotope Cl³⁶ we showed that submersion of fossil teeth in solutions of small ions such as Cl⁻ or hypochlorite, ClO⁻, cause that they migrate right into the pulp. This may lead to the unwanted destruction of authentic DNA. However, using pairs of teeth from the remains of four ancient Europeans (1000–2000 YBP) as well as tooth and hair from an Inuit skull (>300 YBP) we provide evidence that at least some endogenous human fossil DNA survives in powdered pulp/dentin that has been submersed in 2% hypochlorite. Further, we show that powdered pulp/dentin deliberately contaminated with huge amounts of a 414 bp PCR product is effectively decontaminated by suspension in 2% hypochlorite for 5 min. Decontamination of fossil material from teeth may therefore be accomplished by a short direct action of hypochlorite on the powdered specimen rather than less controllable and less efficient external treatments of the whole specimen.     

Hyper or hypo-masculine? Re-conceptualizing ‘hyper-masculinity’ through Seattle’s gay,leather community

This article offers a critique of the concept of hyper-masculinity and a more nuanced, place contingent, critical way to think about masculinity. I use the concept of hyper-masculinity to highlight a conceptual problem between essentialist and de-essentialized notions of gender. Constructionist notions of masculinity (and gender) do not escape the essentialist problem; however, by critiquing and offering a placed conceptualization of hyper-masculinity based on Seattle’s gay leather community, I push the boundaries of masculinity and gender to arrive at a more nuanced, embodied, place-based and contingent understanding of hyper-masculinity thereby side-stepping debates that essentialist/constructionist. Through participant-observations and ethnographic interviews with men who practice hyper-masculinity within the gay leather community in Seattle, USA, I interrogate hyper-masculinity within the community to demonstrate how it reinforces and subverts heterosexual gender roles and homonormativity.     

唐玉门关地望新探——基于历史文献与考古遗存互证

在实地调查的基础上,根据传世典籍和出土文献的记载,结合年代测定、考古发现和遥感影像资料,通过空间分析,作者认为唐玉门关和墨离军驻地应在河西走廊西段的常乐故城,即今瓜州县“六工古城”遗址。这里是丝绸之路的交通枢纽,是西域出入口的重要关卡,亦即北魏时期新开伊吾道的起首。唐代玉门关地处疏勒河和榆林河下游洪积冲积扇缘的古绿洲,地貌和水资源等地理环境因素是制约关城选址的基础条件,而政治、军事等人文地理因素则是决定玉门关时空变迁的关键。     

Genetic characterization of an archaeological sheep assemblage from South Africa’s Western Cape

The Neolithic Revolution, constituting a shift from food acquisition to food production, came to Africa as it did to most of the rest of the world: through processes of transmission rather than through de novo innovation. In contrast with other regions the pastoral management of cattle, sheep and goats was widespread in Africa thousands of years before settled agricultural communities or the use of domesticated plants were in evidence ( 46, 35 and 10). We report here the discovery of haplogroup B in the first genetic analysis of an African archaeological sheep assemblage.     

An overview of bitumen trade in the Near East from the Neolithic (c.8000 BC) to the early Islamic period

The aim of the paper is to summarise the present state of knowledge concerning bitumen trade in the Near East from the Palaeolithic (70,000 BP) to the Early Islamic period. During the Palaeolithic and Early Neolithic period, bitumen utilisation was mostly concentrated in settlements close to oil seeps. From the Ubaid 3 period, bitumen from the Mosul area became more important and was traded as far as the southern Persian Gulf. The Uruk period is a turning point for Mesopotamian history as settlements evolved into city‐states. These cities had a great need for raw materials, and this marks the beginning of large‐scale exploitation of Hit bitumen. This bitumen was traded at settlements along the Euphrates, where a large trade network was established. Hit bitumen entered the Persian Gulf at the turn of the second millennium (Dilmun period). Bitumen from Iraq (Mosul and Hit) became predominantly used in most settlements along the southern coast of the Gulf. During this period Iranian bitumen was also exported and this supply tended to increase, especially during the Partho‐Sasanian period. Dead Sea bitumen had its own exchange network, which was concentrated across present‐day Israel and Egypt where it was extensively used for mummification.     

Neolithic Hedgehogs (Erinaceus europaeus) from the Island of Gotland show early contacts with the Swedish mainland

Previous research probing early migrations and contacts in the Baltic Sea area is characterized by the analysis of different chronologies and subsistent strategies on all sides of the Sea. Several studies performed on artifact typology, ceramics, grave rituals and physical anthropology ended with varying results. Although the question of human origins remains inconclusive, in this study, we rely on the phylogeography of an animal associated with humans to elucidate findings regarding prehistoric human migration and contacts.Hedgehogs, along with other fauna on Gotland, were brought over to the island by humans. We examined hedgehog mitochondrial DNA from the Pitted Ware Culture (Middle Neolithic). The genetic signatures of the animals on the island were investigated to determine the animal’s origin.From the 23 bones originally examined, twelve bones from all five locations studied yielded reliable results and resembled published extant Erinaceus europaeus sequences from Sweden, Norway and Denmark. We postulate that a western heritage for the Neolithic hedgehogs on Gotland indicates early human contact with the Swedish mainland.