Ancient DNA typing shows that a Bronze Age mummy is a composite of different skeletons

Excavations at Cladh Hallan, a Bronze Age-Iron Age settlement on South Uist in the Outer Hebrides off the west coast of Scotland, revealed the skeletons of two adults, a sub-adult and a child buried beneath the foundations of three roundhouses. Osteological and isotopic evidence has shown that the male adult skeleton is a composite made up of parts of at least three different individuals. To test the hypothesis that the female skeleton was also a composite we examined ancient DNA from four of its components: the skull, mandible, right humerus and right femur. Seven polymerase chain reactions (PCRs) were attempted, these covering positions 15,996–16,420, 16–132 and 232–368 of the mitochondrial DNA hypervariable I and II regions. Three PCRs were successful for each sample and a total of 55 sequences were obtained from the cloned products. After exclusion of possible contaminating sequences, the remaining 34 were compared. It was concluded that the mandible, humerus and femur come from different individuals. Insufficient data were obtained to draw conclusions regarding the origin of the skull. The presence of two composite skeletons at Cladh Hallan indicates that the merging of identities may have been a deliberate act, perhaps designed to amalgamate different ancestries into a single lineage.     

深圳大鹏所城将军府第建筑群体特征分析

深圳大鹏所城较完整的保存了清晚期及民国时期的不同建筑群体,其中将军府第建筑具有很高的历史文化、科学艺术等多方面的价值。其建筑形制、结构、装饰装修方面颇具特点:1.平面结合紧凑,类型丰富,组合较灵活,结合了中原地区四合院落殿堂式结构的组合特色,也反映了岭南山地建筑特点;2.多采用密集、方形的平面和空间布局;3.厅堂与天井结合紧密,室内外连通,形成了外封闭、内开敞的建筑空间;4.具有多样的建筑梁架结构,集中了北方建筑的抬梁式构架、南方的穿斗式及岭南建筑常见的梁架做法,表现了南北建筑文化的大融合;5.外观朴实又有岭南地方特色的精细灵巧的内部装修。     

台湾出土部分古代玻璃珠的科学分析

使用北京大学科技考古实验室的激光剥蚀电感耦合等离子体发射光谱分析了台湾省出土的18件古代玻璃珠子标本．结果表明这些珠子主要属于钙镁玻璃系统、钾玻璃系统、铅玻璃系统以及钠钙玻璃系统．除钠钙玻璃以外其它玻璃的制作技术均和同时期在大陆流行的玻璃制作技术同源。而成分分析结果显示钠钙玻璃可能来自印度或者东南亚,说明中古时期台湾岛是“海上丝绸之路”的重要中转站。     

A DNA test for sex identification in cattle confirms osteometric results

It is of vital importance to be able to sex identify cattle remains to understand the strategies and importance of cattle husbandry in an ancient society. This is usually done from osteoarchaeological assemblages and often relies on measurements of metapodials. The breadth measurement of the distal trochlea is considered an easy way to identify the sex. Bones from males appears to be easily distinguishable from female counterparts, although it has been complicated to find an external control for the morphological results. Here we investigate the reliability of these particular morphometrics for sex identifying cattle bones with molecular genetics. We use a sex discriminating single nucleotide polymorphism in the ZFXY gene and we apply it to DNA from the bones. To keep the fragment size short and suitable for ancient DNA we base the test on a SNP. The test confirms the osteological sex identification in all cases were DNA could be retrieved. This molecular method can also be used when no fragments suitable for osteological sex identification can be found or when the measurements are non-conclusive.     

On the elimination of extraneous DNA in fossil human teeth with hypochlorite

Elimination of extraneous DNA in fossil specimens is of paramount importance for the successful isolation and analysis of authentic DNA; this is especially true when the specimens are of human origin. Bones and teeth are commonly decontaminated with bleach containing the powerful oxidising hypochlorite ion. The procedures involve either submersion in or wiping with the chlorine agent. Using the radioactive isotope Cl³⁶ we showed that submersion of fossil teeth in solutions of small ions such as Cl⁻ or hypochlorite, ClO⁻, cause that they migrate right into the pulp. This may lead to the unwanted destruction of authentic DNA. However, using pairs of teeth from the remains of four ancient Europeans (1000–2000 YBP) as well as tooth and hair from an Inuit skull (>300 YBP) we provide evidence that at least some endogenous human fossil DNA survives in powdered pulp/dentin that has been submersed in 2% hypochlorite. Further, we show that powdered pulp/dentin deliberately contaminated with huge amounts of a 414 bp PCR product is effectively decontaminated by suspension in 2% hypochlorite for 5 min. Decontamination of fossil material from teeth may therefore be accomplished by a short direct action of hypochlorite on the powdered specimen rather than less controllable and less efficient external treatments of the whole specimen.     

Ancient DNA in human bones from Neolithic and Bronze Age sites in Greece and Crete

Attempts were made to detect ancient DNA (aDNA) in samples of 88 human skeletons from eight Neolithic and Bronze Age sites in Greece and Crete. Ancient DNA was absent in specimens from Nea Nikomedia, Lerna, Karaviádena (Zakro), Antron Grave Circle A and Mycenae Grave Circle A. For each of three skeletons from Antron Grave Circle B that were sampled, polymerase chain reactions (PCRs) gave products for nuclear but not mitochondrial DNA, but amplicon yield was low and inconsistent with replicate PCRs failing to give reproducible results. With specimens from Mycenae Grave Circle B, evidence for mitochondrial aDNA was obtained for four of the 22 skeletons that were studied, and at Kouphovouno evidence for mitochondrial and/or nuclear aDNA was obtained with eight of the 20 skeletons that were examined. We conclude that, although aDNA might be present in some Eastern Mediterranean skeletons from later centuries of the Bronze Age, it is not commonly found in material from this period and is likely to be absent from older material.     

The survival of PCR-amplifiable DNA in cow leather

We have investigated the survival of PCR-amplifiable mitochondrial and nuclear DNA in a small number of modern and medieval bovine leather samples. The results of this preliminary investigation demonstrate that, while no nuclear DNA can be PCR-amplified from any of the specimens, mitochondrial DNA can be amplified from all samples. To investigate this contrasting pattern of DNA survival further, we have vegetable-tanned cow skin in our own laboratory, and directly assayed the survival of PCR-amplifiable mitochondrial and nuclear DNA at each step of the process. The results indicate that nuclear DNA is reduced to sub-amplifiable levels as a result of the tanning baths, whereas amplifiable mitochondrial DNA survives the complete process. Our results suggest that old and archaeological bovine leather may represent a useful source of genetic information, although this information will most likely be limited to that which can be gained from mitochondrial DNA.     

徽派版画中的名作《古歙山川图》作者考

本文对徽派版画中的《古歙山川图》的作者做了比较详尽的考证。     

Assessing Conflict and Exchange: The Defensive and Access Layout of La Alumbrera (c. AD 1000–1480, North-west Argentina)

Abstract

The Regional Development Period (c. ad 1000–1480) in north-west Argentina is characterized, among other important features, by the coexistence of two phenomena seemingly opposed: a constant situation of violent conflict between communities and the maintenance or even intensification of interregional goods exchange networks. Although recent studies recognize the simultaneity of these processes, only a few scholars asked how communities dealt with the necessity of protecting themselves as well as obtaining distant resources. In this paper we present the analysis of the defensive and access layout of La Alumbrera archeological site which has the peculiarity of condensing features that may illustrate an example of how conflict and exchange coexisted at a local scale.