Insights into the processes behind the contamination of degraded human teeth and bone samples with exogenous sources of DNA

A principal problem facing human DNA studies that use old and degraded remains is contamination from other sources of human DNA. In this study we have attempted to contaminate deliberately bones and teeth sampled from a medieval collection excavated in Trondheim, Norway, in order to investigate this poorly understood phenomenon. Five pairs of teeth and bone samples were bathed in water containing various concentrations (from 10⁻⁹ and 10⁻²¹ g/l) of purified ΦX174 DNA. Subsequently the samples were subjected to a routine decontamination protocol involving a bleach bath followed by exposure to λ=254 nm ultraviolet light, prior to DNA extraction and analysis for evidence of the persistence of the contaminant. The results support previous speculation that bone is more susceptible to water‐borne sources of contaminant DNA, although both bone and teeth are readily contaminated and are difficult to decontaminate using the tested protocol. We believe that this is largely due to the porous nature of bone and teeth facilitating the deep penetration of the contaminant DNA. To simulate a more realistic handling situation, 27 further teeth were directly handled and washed, then decontaminated, prior to assaying for the residual presence of the handler's DNA. Surprisingly, although our results suggest that a large proportion of the teeth were contaminated with multiple sources of human DNA prior to our investigation, we were unable to contaminate the samples with further human DNA. One potential explanation may be the deposition of sediment or other structural changes that occur within the samples as they desiccate post‐excavation, which may protect samples from subsequent contamination, but also prevent the efficacy of bleach baths in decontaminating specimens. Copyright © 2006 John Wiley & Sons, Ltd.     

Rehabilitating feminist politics and political theatre: Hélène Cixous's La Ville parjure ou le réveil des Erinyes at the Théâtre du Soleil

Hélène Cixous's 1994 play La Ville parjure ou le réveil des Erinyes , first produced at Ariane Mnouchkine's Théâtre du Soleil , insists on both theatre and feminism as valuable political practices in the face of the AIDS pandemic. Disguised in the trappings of Greek tragedy, the play reframes the scandal over the French government's complicity in the distribution of blood contaminated with HIV. As the mother of two young plague victims openly exhorts the audience to take part in righting social wrong, the Furies wreak havoc on the on-stage regime that ignores its citizens suffering. Ultimately the emphasis is not on peaceful resolution but on the critical need for action on social issues. The maternal heroine and strong women figures in this elaborate production insist on the impossibility of divorcing aesthetic considerations of theatre from its responsibility to create public awareness.     

Practical and Auspicious: Thai Handbook Knowledge for Agriculture and the Environment

This article is the first thorough examination of the Thai handbooks that are produced to explain agricultural and environmental knowledge. These khu-meu (handbooks) and tamra (textbooks) come into use when knowledge is moving from one party to another. They also establish symbolic correlations within the human, terrestrial world, or between the human, terrestrial world and the worlds of the gods, spirits, ancestors, or the unseen. Despite the fact that handbooks are pervasive for the organising, preserving, retrieving, transmitting and consuming of knowledge throughout the Southeast Asian region, there has been very little concerted study of handbook knowledge. Our analysis of environmental handbooks in Thailand shows that simplified mastery is a common goal of the handbook genre in both its “how to” and “reading signs” forms. The knowledge captured by the Thai language handbooks is of a practical, predictive kind and suited to particular circumstances. Such knowledge can ultimately bridge and blur the dichotomy between scientific and local epistemologies.     

Poverty in Depth: a New Dialogue

This reflective piece draws together the themes and issues presented within the volume, exploring historic and contemporary definitions and attitudes towards poverty and their implications of the archaeological study of “slum” neighborhoods. It compares and contrasts the individual case studies from York and Manchester with investigations in America and Australia, drawing attention to the differences between them. Suggestions are made for future investigations, particularly in the potential for further comparative work at an international level.     

Mimetic Structuration: Or,Easy Steps to Building an Acceptable Identity1

Since their settlement two millennia ago the Comoro Islanders have been subjected to a diversity of external influences that have provoked significant and often radical social change. This article develops the concept of mimesis to explain how the Comoros have deal with these influences, incorporating and developing them to create cultural and social structures that may appear eclectic but which have equipped the islanders to negotiate these changes quite effectively. As a result of their mimetic praxis, Comorians confront and manage influences both internal and external with the minimum of social upheaval, maintaining a society that is both highly heterogeneous and remarkably enduring.