Variable nucleotide tandem repeat (VNTR) typing of two palaeopathological cases of lepromatous leprosy from Mediaeval England

Using ancient DNA methods, we have examined in detail two archaeological cases of leprosy from Mediaeval England. The first was a child skeleton with rhino-maxillary changes typical of lepromatous leprosy (LL). The second case was the skeleton of a male adult who showed both typical rhino-maxillary changes and osteitis/periostitis on the leg and foot bones. Bone powder was sampled from both cases and DNA extracts were prepared. These were subjected to a series of polymerase chain reactions (PCRs) specific for regions on the Mycobacterium leprae genome. The repetitive element RLEP was used for confirmation of M. leprae DNA and then three polymorphic regions were successfully amplified and sequenced to determine the number of variable nucleotide tandem repeats (vntr) at these loci. These were the microsatellite regions ML2344 and ML2172 and the minisatellite region ML0058. Genotyping data from the strains preserved within the skeletal remains were compared with those obtained for a reference strain of M. leprae. Variation at these three loci was found between both burials and the reference strain, indicating that vntr typing of LL cases from the archaeological record is a useful way of confirming disease and an additional means of authenticating aDNA data. This demonstrates the feasibility of targeting multiple loci for phylogenetic studies of leprosy strains from archival sources.     

General practice access in regional and remote Australia for ageing populations

Identifying and removing access barriers to the timely provision of comprehensive health care is increasingly important for the wellbeing of Australia's rapidly ageing and frail populations, particularly those in non‐metropolitan settings. This study has examined if current general practice (GP) locations in non‐metropolitan South Australia (SA) and Western Australia (WA) are geographically accessible to the rapidly growing frail and prefrail populations known to have a high level of health service use and reduced mobility. Geospatial analysis linking 60‐kilometre GP service catchments, 2016 population counts, and 2027 population projections has estimated that the size of the frail and prefrail population that live outside these GP service catchments will double, reaching 7,800 people by 2027. The maldistribution of GP locations was most evident in WA. As regional and remote populations continue to age, the challenge of health service provision, including geographic access to care, must be resolved to ensure that populations in these areas have the best opportunity to age well. Geospatial methods linking service and demographic information, such as the approach used in this analysis, can aid in planning the equitable provision of health care for older Australians.     

Outsourcing the Business of Development: The Rise of For-profit Consultancies in the UK Aid Sector

While much attention has been paid to the ways in which the private sector is now embedded within the field of development, one group of actors — for-profit development consultancies and contractors, or service providers — has received relatively little attention. This article analyses the growing role of for-profit consultancies and contractors in British aid delivery, which has been driven by two key trends: first, the outsourcing of managerial, audit and knowledge-management functions as part of efforts to bring private sector approaches and skills into public spending on aid; and second, the reconfiguration of aid spending towards markets and the private sector, and away from locally embedded, state-focused aid programming. The authors argue that both trends were launched under New Labour in the early 2000s, and super-charged under successive Conservative governments. The resulting entanglement means that the policies and practices of the UK government's aid agencies, and the interests and forms of for-profit service providers, are increasingly mutually constitutive. Amongst other implications, this shift acts to displace traditional forms of contestation and accountability of aid delivery.     

A Mediaeval Case of Lepromatous Leprosy from 13–14th Century Orkney, Scotland

Erosion in the 1960s resulted in exposure of human skeletal remains from a Norse Christian cemetery at Newark Bay, Orkney, Scotland. One set of remains showed osteological evidence of advanced lepromatous leprosy, but the absence of bones from the lower limbs precluded definitive diagnosis. The aim of the present study was to determine whether Mycobacterium leprae could be detected in bone extracts, as a means of confirming the diagnosis of leprosy. Bone samples were examined from the suspected leprosy case and from a second contemporary burial thought to be free of disease. DNA was amplified by polymerase chain reaction (PCR) using primers specific for a repetitive element (RLEP) characteristic of M. leprae. Additional PCR tests specific for Mycobacterium tuberculosis and for amelogenin (a human gene suitable for sex determination) were also applied to the samples. M. leprae DNA was detected only in the skull sample from the suspected leprosy case. The DNA sequence was identical to that found in present day isolates of M. leprae. Positive results were obtained only using a PCR reaction designed to amplify relatively short stretches of DNA (<175 bp), suggesting the microbial DNA had undergone extensive fragmentation. There was no evidence of M. tuberculosis DNA in bones from the leprosy suspect or control individual. The ability to recover ancient samples of DNA provides an opportunity to study long-term evolutionary changes that may affect the epidemiology of microbial pathogens.     

Citizen participation,community resilience and crisis-management policy

This article presents two arguments. The first relates to the relevance of citizen participation vis-à-vis the design and implementation of public policy. More specifically, the article empirically demonstrates how a model of community decentralisation can have a number of practical benefits for crisis-management policy. The second argument relates to a question that has come to characterise studies of citizen participation in public policy. Why is it that there is so much rhetoric in support of participation but so little action in terms of the day-to-day realities of policy implementation? We place this question in a crisis-management context so that we might ask: why is it that crisis-management systems built around the principles of community resilience continue to fail on these very grounds? We find our answer to this question in state-centric governance settings which devolve authority, but do not relinquish it.

本文提出两个观点。第一个观点涉及公民参与对于公共政策设计和实施的意义。具体地说,本文用实证的方法说明社区分权模式如何对危机管理政策产生一系列实际效益。第二个观点涉及公民参与公共政策的问题：为什么对公民参与口头上不吝支持,而日常政策的实施上却不见动静？这个问题可以放在危机管理的语境中重新提问：为什么围绕着社区弹性原则的危机管理体系仍以这样的理由失效？我们的答案是：以政府为中心的治理环境虽然转移但并未放弃权威。     

Single-Degree-of-Freedom Characterization of Multi-Phase Passive Control Systems

A Multi-phase Passive Control System (MPCS) combines two passive control devices in order to offset the individual weaknesses and enhance structural performance, allowing the structure to respond effectively to varying levels of loading. Previous work involving a nine-story frame demonstrated the effectiveness of MPCSs but the fundamental understanding of the system was lacking. In order to more clearly understand the behavior and identify important parameters and parameter interactions, a single-degree-of-freedom (SDOF) non-linear dynamic study was performed. The results offer significant insight towards developing structural systems adaptable to multi-objective performance-based design procedures that meet higher performance standards than for ductility-based design.     

Mapping Submerged Landscapes Using Multibeam Bathymetric Data: a case study from the north coast of Ireland

This paper outlines work currently being undertaken to reconstruct submerged archaeological landscapes off the north coast of Ireland. This research uses the recently‐completed Joint Irish Bathymetric Survey (JIBS), which has provided full‐coverage high‐resolution multibeam bathymetric data for the sea‐bed off the north coast. This data has been examined for evidence of past sea‐level change and been used to produce palaeo‐geographic reconstructions of the past landscape, which in turn have facilitated the identification of ten areas of high archaeological potential. The results presented here will form the basis of a future programme of archaeological survey and prospection. © 2010 The Authors     

New light on the personal identification of a skeleton of a member of Sir John Franklin’s last expedition to the Arctic, 1845

In 1845, an expedition, commanded by Sir John Franklin, set out to try and discover the north-west passage. All 129 men on this ill-fated voyage perished. Over the years, skeletal remains associated with the final throes of the expedition have been located on and near King William Island, Nunavut, in the Canadian arctic. In general, even a tentative personal identification for these remains has proved impossible. An exception is some skeletal remains that were recovered in 1869 and brought back to England and interred beneath the memorial to the Franklin expedition in Greenwich. In the 19th century, these were tentatively identified as of one of HMS Erebus’s lieutenants, Henry Le Vesconte, a conclusion that has been widely accepted in studies of the Franklin voyage. Renovations to the monument in 2009 provided an opportunity for scientific examination of the remains, and to re-evaluate the personal identification made nearly 140 years before. The current work, which is the first modern scientific analysis of a fairly complete skeleton associated with the Franklin voyage, describes the remains and the artefacts interred with them, discusses the pathological conditions present, and evaluates the personal identification using osteological techniques and isotope geochemistry. Results indicate that the remains are of an adult male of European ancestry. Although some writers have suggested that scurvy or tuberculosis may have been important causes of morbidity and mortality on the Franklin expedition, osteological analysis and, in the case of tuberculosis, DNA analysis, provided no evidence for their presence in these remains. Isotopic studies indicate that the personal identification as Le Vesconte is unlikely to be correct. From the isotopic results and forensic facial reconstruction, HDS Goodsir, an assistant surgeon on the expedition, appears a more likely identification, but the results do not allow a firm conclusion.