Rice archaeological remains and the possibility of DNA archaeology: examples from Yayoi and Heian periods of Northern Japan

Japanese rice cultivation in paddy fields has 2,400∼3,000 years of history. Most of modern Japanese rice varieties are classified as Temperate-japonica (Tm-J). Few landraces are recognized as Tropical-japonica (Tr-J) only in southwestern Japan. However, ancient DNA studies and phytolith analysis suggest that Tr-J strains were more popular in the past than now. Maekawa is a complex archaeological site composed of paddies dated from the Yayoi (2,100 years BP) to the Heian (1,100 years BP) periods. Phytolith analysis indicates that intensive rice cultivation was practiced in both periods, but there was no cultivation in the intervening period. Morphological features of bulliform phytoliths suggest that Tr-J was cultivated during both periods. Locally, rice cultivation during the Heian period was brought to a close by a flood event, in which immature rice plants were pulled down and buried in silt to be preserved in a quasi-carbonized/ waterlogged state. Ancient DNA (aDNA) analysis of the carbonized plant culm from Heian Maekawa recovered chloroplast DNA sequences of the 6C7A plastid subtype, which is common to both Tr-J and Tm-J, whereas two plastid subtypes, such as 6C7A and also 7C6A, were found in aDNA of carbonized grains from the Tareyanagi site of the Yayoi period. The latter plastid subtype was specific only to Tr-J. In order to better characterize the past rice populations, modern landraces collected in the local area were classified with morphophysiological traits. Some of the landraces were found to carry several traits of Tr-J, including bulliform phytolith types, but mixed with Tm-J traits. Based on the discontinuous distribution of rice phytoliths between the Yayoi and the Heian period, the early introduction of rice cultivation may have been discontinuous and locally reintroduced after a ∼1,000-year hiatus, but with a genetically different rice population. Such populations were composed from Tr-J like strains as shown by landraces but with reduced diversity in plastid types. Through such changes, since the Yayoi era, Tr-J was largely replaced by Tm-J, although ancient Tr-J continued to participate in the genetic makeup of later rice populations and may have aided the local adaptation of introduced Tm-J.     

Errors Caused by Rounded Data in Two Simple Facility Location Problems

As a rule, data to be used in locational analysis are either rounded up or rounded down. Therefore, error is incurred if such location data are used. The objective of this paper is to examine location error and cost error due to rounding in unweighted minisum and minimax problems in one-dimensional continuous space. Several conclusions on rounding effects are obtained by examining the respective mean-squared errors. First, rounding tends to exert more serious influence on the minisum problem than on the minimax problem. Second, in both location problems, the location error shows a pattern that is the inverse of that of the cost error.     

A strontium isotope analysis on the relationship between ritual tooth ablation and migration among the Jomon people in Japan

Distinct patterns can be discerned in the extensive ritual tooth ablation found among the human skeletal remains of the Late–Final Jomon period (ca. 3200–2800 cal BP) in Japan. Based on comparative observations of sex and grave patterns in the skeletal remains, two major patterns in ritual tooth ablation, termed type 4I and type 2C, have been assigned to locals and immigrants, respectively. In order to test this hypothesis, strontium (Sr) isotope (⁸⁷Sr/⁸⁶Sr) analyses were performed on human skeletal remains from the Yoshigo shell mound in Aichi Prefecture, central Japan. Plants in the surrounding area were also examined to illustrate the geographic ⁸⁷Sr/⁸⁶Sr distribution. The Sr isotopic variation in human tooth enamel (⁸⁷Sr/⁸⁶Sr = 0.70868–0.71028) was greater than that in human bones (⁸⁷Sr/⁸⁶Sr = 0.70871–0.70943). Individuals with higher Sr isotope ratios in their tooth enamel than seawater Sr values of 0.7092 can be identified as immigrants (36% of population). The presence of these isotopically identified immigrants between both type 2C and type 4I individuals does not support the previous hypothesis. The intra-population ⁸⁷Sr/⁸⁶Sr distribution of tooth enamel of type 2C individuals showed a significantly higher mean ratio than that of type 4I individuals, suggesting a higher proportion of immigrants among the former.     

Changes in shell gathering in an early agricultural society at the head of Ise Bay,Japan

We examined changes in shell gathering in an early agricultural society at the head of Ise Bay, Japan based on the analysis of almost 20,000 individual shell remains from four coastal settlements. We analyzed historical changes in the composition and size range of the shell species Meretrix lusoria. The most common species went from Crassostrea gigas in the early Middle Yayoi period (about 400–350BC) to M. lusoria in the mid-Middle Yayoi period (about 320–200BC). It is possible that the seacoast tideland, an environment suitable for C. gigas, was buried in sediment from the rivers and thereby transformed into muddy sand bottoms, an environment more suitable for M. lusoria. We also analyzed historical changes in the size range of M. lusoria. The mean size of M. lusoria increased significantly from the early Final Jomon (about 1250–1000BC) to the late Early Yayoi (about 500–400BC), from the late Early Yayoi to the early Middle Yayoi, and from the early to the mid-Middle Yayoi periods. Evidence suggests that during the Yayoi period, the demand for shells decreased and therefore, the pressure to collect M. lusoria also decreased. We concluded that the larger specimens of M. lusoria were collected selectively.     

Salt weathering of sandstone at the Angkor monuments,Cambodia: identification of the origins of salts using sulfur and strontium isotopes

The Angkor monuments in northwestern Cambodia, which are primarily made of sandstone, are suffering from deterioration due to salt weathering. In order to elucidate the sources of the salts and salt weathering process, this paper analyzed bulk chemical compositions and S (sulfur) and Sr (strontium) isotopic ratios for the salts and surrounding environmental materials. At places where bats inhabit the structures, various kinds of sulfate and phosphate salts are formed on the surface of the sandstone. The results of the analysis demonstrate that the S and P (phosphorus) components in the salts are primarily derived from bat guano. Moreover, Ca (calcium), which is the major element in the salts, is suggested to be derived from both bat guano and the sandstone. The isotopic data strongly suggests that bat guano is related to salt weathering. Removal of these animal excrements is essential for future conservation of these monuments. On the other hand, exfoliation of sandstone commonly occurs due to crystallization of calcite (CaCO₃) on platform surfaces of pyramid-type monuments. A large amount of Ca in calcite is suggested to be derived from calcite cement of the building sandstone.     

Early Maya Ritual Practices and Craft Production: Late Middle Preclassic Ritual Deposits Containing Obsidian Artifacts at Ceibal,Guatemala

This article examines Preclassic Maya ritual practices and craft production by means of a study of ritual deposits containing obsidian artifacts dated mostly to the late Middle Preclassic period (700–350 b.c.) at Ceibal, Guatemala. New ritual practices developed at Ceibal during this period, possibly through political interactions and negotiation involving emerging elites and other diverse community members. Common objects in ritual deposits in the public plaza shifted from greenstone celt caches to other artifacts, including those made of obsidian. The inhabitants of Ceibal engaged in various kinds of craft production, including the manufacture of obsidian prismatic blades. They also conducted public rituals in the Central Plaza, depositing exhausted polyhedral obsidian cores and other artifacts with symbolic significance in caches and as offerings in incipient elite burials and interments of sacrificed individuals. These cores clearly demonstrate the use of a sophisticated blade technology. Like greenstone objects, exhausted polyhedral obsidian cores deposited in cruciform arrangements along the east–west axis of the central E-Group plaza were used as symbols and markers of the center and four cardinal directions within the Maya cosmos. Public rituals were important for creating collective identities and for processes of political negotiation within the community. Emerging elites likely came to play an increasingly important role in public rituals as principal performers and organizers, setting the stage for later public events centered on rulers.