Persistence of birefringence and specific collagen cross-links in dentine of teeth of the ‘Nuraghi’ population living in Sardinia BC 1500–1200

Well preserved bones of the Nuraghi population, discovered in 1980, are deposited in the Department of Biomedical Sciences, Division of Human Anatomy of the University of Sassari, in Sardinia, Italy. The Giant Tomb belongs to the megalithic monuments constructed in the period BC 1500–1200. The origin, history and the fate of Nuraghi population is not well known. Many cone-shaped fortresses are found in Sicily and megalithic monuments for burial purposes were built in their proximity. Due to the good macroscopic preservation of the mineralized parts of the Nuraghi skeletons we decided to compare the histological and biochemical structure of the recovered Nuraghi teeth with contemporary ones. Crown dentine of four Nuraghi molar and four premolar teeth were analysed and compared with contemporary molars and premolars removed for orthodontic reasons from 17–40-year-old patients in the Dental School, University of North Carolina at Chapel Hill USA. Evaluation of the collagen cross-links of dentine collagen was performed using a HPLC technique to analyze for hydrolysates. Nonreducible, stable cross-links pyridinoline and its lysyl analogue were quantified by fluorescence (excitation 330 nm, emission 390 nm). The concentration of reducible, labile cross-links referred to as DHLNL and HLNL (dehydro-dihydroxylisinorleucine) were identified and calculated by chromatography after the application of NaB³H₄ with integration of respective peaks and converting the numbers into a residue per mole of collagen basis. The ultrastructure of collagen was estimated by its persistent birefringence in polarized light after staining with Picrosirus Red. It was found that dentine collagen of Nuraghi teeth resisted environmental factors sufficiently well to preserve the molecular ultrastructure of collagen fibres, proved by their birefringence and by the preservation of some of the stable and reducible collagen cross-links, characteristic for skeletal tissues. © 1998 John Wiley & Sons, Ltd.     

Combined application of pansharpening and enhancement methods to improve archaeological cropmark visibility and identification in QuickBird imagery: two case studies from Apulia,Southern Italy

Launch of Very High Resolution (VHR) satellite sensors meant a significant evolution for Remote Sensing archaeological applications since data with high spatial resolution may be used to identify signals related to buried archaeological remains (e.g. cropmarks, soilmarks, dampmarks) in very extended territories. The aim of this work is to develop a suitable image processing workflow to improve the cropmark visibility in the VHR satellite images. The workflow implemented consists of two steps: first the panchromatic and multispectral images have been pansharpened in order to merge the two images, then some Enhancement methods have been applied on the pansharpened image. As a result of the several tested pansharpening techniques, the Principal Component Resolution Merge yields images of very good quality useful not only for visualization but also as input for further processing. Among the different enhancement methods taken into consideration, the Spatial Enhancement, carried out by applying the Wallis Adaptive filter further improve the visibility and recognition of the archaeological signals.     

Obsidian from Pulo di Molfetta (Bari,Southern Italy): provenance from Lipari and first recognition of a Neolithic sample from Monte Arci (Sardinia)

Twenty-four obsidian artefacts of the Neolithic Age were found at the large karstic doline of Molfetta (near Bari) along the Southern Italian Adriatic coast. Non-destructive chemical analyses of the glass, by SEM-EDS, allowed their source rock regions to be determined. Twenty-three of the studied artefacts had been traded from the island of Lipari. Only one sample had a glass composition and microphenocrysts (biotite, pyroxene and feldspar) that indicate provenance from the Monte Arci area of Sardinia, in particular from the Perdas Urias (SC) outcrops. The attribution of this artefact to Sardinia enlarges the geographical pattern of Monte Arci obsidian exploitation and distribution from island to Southern Italy during the Middle to Late Neolithic.     

Sclerosing Bone Dysplasia from 16th Century Sardinia (Italy): A Possible Case of Camurati–Engelmann Disease

The skeletal remains of a male aged 45–55 years displaying several bone anomalies were unearthed from the Alghero (Sardinia) plague cemetery ‘lo Quarter’, a burial site dating back to the 1582–1583 AD outbreak. The skeleton, whose stature is about 165 cm, presents a bilateral hyperostosis with increased diameter of the diaphyses of all the long bones of the upper and lower limbs; the metaphyses appear to be involved, while the epiphyses are spared. Marked thickening of the cranial vault is also evident. Radiological study showed irregular cortical thickening and massive endoperiosteal bone apposition; sclerotic changes are observed in the diaphysis of some metacarpals. Computed tomography (CT) cross sections of the long bones displayed a thickening of the cortical portion and endoperiosteal bone apposition. The individual was affected by a sclerosing bone dysplasia, a genetic disease characterized by increased bone density. In differential diagnosis, several sclerosing bone dysplasia, such as hyperostosis corticalis generalisata, craniodiaphyseal dysplasia, craniometadiaphyseal dysplasia, pachydermoperiostosis and Camurati–Engelmann disease, as well as other disorders characterized by sclerosing manifestations, such as Erdheim–Chester disease, mehloreostosis and skeletal fluorosis, need to be considered. The anomalies observed in skeleton 2179 fit with the features of Camurati–Engelmann disease, which is the most likely candidate for final diagnosis. It is highly challenging to evaluate how such a condition may have influenced the individual's lifestyle in terms of development, mobility and quality of life. This individual was probably symptomatic and must have experienced common clinical symptoms, such as pain in the limbs and fatigability. However, the strong development of the muscular insertions and the degenerative changes in the upper limbs suggest that the mobility problems should not have prevented him from reaching a mature age and from performing essential daily activities. The presented case is the unique paleopathological evidence of Camurati–Engelmann disease so far diagnosed. Copyright © 2015 John Wiley & Sons, Ltd.     

A study by thermal treatment and X-ray powder diffraction on burnt fragmented bones from tombs II,IV and IX belonging to the hypogeic necropolis of “Sa Figu” near Ittiri,Sassari (Sardinia,Italy)

In the course of an anthropological investigation of three tombs of the hypogeic necropolis of “Sa Figu” (near the village of Ittiri in North Sardinia, Italy), numerous burnt bone fragments were collected. In particular, from the whole necropolis we selected a tenth of long bones from tomb IV, a small fragment of femur from tomb II and four fragments belonging to tomb IX. The aim of this work is to determine a temperature range to which the bone fragments were heated, in order to assess a funerary cremation which was presumably used at that time to quickly and hygienically eliminate the flashy parts of deceased people rather than a purification rite with fires inside the sepulchres. We attempted to evaluate the range of temperature to which the bones were likely subjected, making a joint use of thermal treatment and powder X-ray diffraction investigations. From our X-ray line broadening results carried out with a modern approach (Rietveld method), it emerged that a group of five fragmented burnt bones (one specimen belonging to tomb IV, the other four from tomb IX) were subjected to a heat treatment in a temperature range from 600 and 750 °C. Conversely another group of bones (belonging to tombs II and IV) turned out to be subjected to temperatures not higher than 250 °C.     

Anthropological and physicochemical investigation of the burnt remains of Tomb IX in the ‘Sa Figu’ hypogeal necropolis (Sassari,Italy) – Early Bronze Age

Excavations carried out in Tomb IX of the hypogeic necropolis of ‘Sa Figu’, near the village of Ittiri (Sassari, Italy), supplied burnt human bone remains and pottery unambiguously referred to the Early Bronze Age (characterised by the local culture of ‘Bonnannaro’). Besides the anthropological study, we have investigated and evaluated the possibility of a funerary cremation practice in Sardinian pre‐history, a subject that has previously not been considered from a scientific point of view. Making use of a calibration procedure based on X‐ray diffraction (XRD) line‐broadening analysis, related to the microstructural properties, it was possible to estimate the combustion temperature to which the fragmented bones were subjected. It was found that the studied bones reached temperatures varying from 400°C up to a maximum of 850°C. This spread of values suggested inhomogeneous combustion of the bones, which seems compatible with funerary cremation practices. Copyright © 2007 John Wiley & Sons, Ltd.