Extraction and sequencing of human and Neanderthal mature enamel proteins using MALDI-TOF/TOF MS

We report here the first results of a method to extract and sequence mature enamel proteins from modern human and Neanderthal tooth enamel. Using MALDI-TOF/TOF mass spectrometry and a combination of direct sequencing and peptide mass mapping we have sequenced a peptide from the tyrosine-rich amelogenin peptide (TRAP) of the X isoform of the amelogenin protein for modern and recent human samples. We also report our results from two Neanderthal enamel samples where we were also able to recover fragments of the TRAP protein, which had a similar sequence to the modern human samples.     

Response to Nowell and Horstwood (2009)

Here we respond to the comment by Nowell and Horstwood (2009) written in response to our 2008 paper where we presented the first results of the application of laser ablation strontium isotope analysis to a fossil hominin. Although we are pleased that the paper has attracted so much interest, including from researchers outside the field such as Nowell and Horstwood, we completely disagree with the two main arguments of their comment. Namely, we reject the notion that they can recalibrate our data using different methods and (non matrix-matched) standards determined in a different laboratory, and that is simply irresponsible to advocate the further development of this method for rare and important hominin samples through drilling rather than the almost non-destructive method of laser ablation that we presented in our paper.     

Origin of palaeofluids in a normal fault setting in the Aegean region

The province of Burdur (SW Turkey) is seismically an active region. A structural, geochronological, petrographical, geochemical and fluid inclusion study of extension veins and fault‐related calcite precipitates has been undertaken to reconstruct the palaeofluid flow pattern in this normal fault setting in the Aegean region. A palaeostress analysis and U/Th dating of the precipitates reveals the neotectonic significance of the sampled calcites. Fluid inclusion microthermometry of calcites‐filling extension veins shows final melting temperatures (T_m ice) of 0°C. This indicates pure water, most likely of meteoric origin. The oxygen isotope values (?9.8‰ to ?6.5‰ VPDB) and the carbon isotopic composition (?10.4‰ to ?2.9‰ VPDB) of these calcites also show a near‐surface meteoric origin of the fluid responsible for precipitation. The microstructural characteristics of fault‐related calcites indicate that calcite precipitation was linked with fault activity. Final melting temperature of fault‐related calcites ranges between 0 and ?1.9°C. The oxygen isotope values show a broad range between ?15.0‰ and ?2.2‰ VPDB. Several of these calcites have a δ¹⁸O composition that is higher or lower than the oxygen isotopic composition of meteoric calcites in the area (i.e. between ?10‰ and ?6‰ VPDB). The δ¹³C composition largely falls within the range of the host limestones and reflects a rock‐buffered system. Microthermometry and stable isotopic study indicate a meteoric origin of the fluids with some degree of water–rock interaction or mixing with another fluid. Temperatures deduced from microthermometry and stable isotope analyses indicate precipitation temperatures around 50°C. These higher temperatures and the evidence for water–rock interaction indicate a flow path long enough to equilibrate with the host–rock limestone and to increase the temperature. The combined study of extension vein‐ and fault‐related calcite precipitates enables determining the origin of the fluids responsible for precipitation in a normal fault setting. Meteoric water infiltrated in the limestones to a depth of at least 1 km and underwent water–rock interaction or mixing with a residual fluid. This fluid was, moreover, tapped during fault activity. The extension veins, on the contrary, were passively filled with calcites precipitating from the downwards‐migrating meteoric water.     

Measuring prehistoric mobility strategies based on obsidian geochemical and technological signatures in the Owens Valley,California

We compare the organization of obsidian flaked stone technologies in two different time periods at CA-INY-30, a village site in southern Owens Valley, eastern California. Previous archaeological studies suggest a reorganization in settlement patterns between the Newberry (ca. 3500–1500 BP) and Marana (ca. 650-contact) periods, from a highly mobile to a more residentially sedentary one. New geochemical data, based on laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) analyses of obsidian artifacts associated with discrete house floors, support this basic settlement model, but reveal new detail in how people moved across the landscape and accessed, extracted, reduced and used obsidian resources. In the earlier Newberry period, there is no relationship between flake size and distance-to-source, and the falloff curve relating frequency of obsidian against distance is more gradual, as expected, but contrary to our expectations, source diversity is not higher. These factors suggest extremely high mobility, but also selective extraction of particular sources. Newberry obsidian may have been acquired by groups of hunters who embedded quarrying within long-distance trips to distant hunting grounds, and subsequently transported bifacial cores to base camps. By contrast, Marana patterns show strong relationships between flake size and distance from source and steeper fall-off curves, suggesting groups acquired their obsidian primarily from closer sources, likely via exchange networks. At the same time, geochemical diversity, especially among smaller resharpening flakes, is higher in the Marana period, highlighting the wide-ranging conveyance systems through which obsidian moved.     

Linguistic boundaries as barriers to exchange

Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) data are used to explore the structure of obsidian acquisition for groups in the King Range National Conservation Area of northern California. The data indicate that, contrary to a distance-decay model, obsidian from more distant sources in the Medicine Lake Highlands, dominates the obsidian found at sites in the area. Furthermore, metric data indicate a positive relationship between size and distance to source with more distant sources represented by larger flakes. It is suggested that this pattern is the result of culturally mediated exchange relations under which Athabaskan speakers concentrated trade relations with one another to the exclusion of trade with linguistic groups to the south, with longer histories in the area, such as the Pomo and Yuki. Additionally, the use of redwood dugouts on rivers in northern California and the importance of northeastern Californian obsidian in ritual practices of California Athabaskan groups may have further encouraged the use of more distant, northeastern Californian, sources.     

Isotopic dietary reconstruction of humans from Middle Bronze Age Lerna,Argolid, Greece

This study presents the results of a carbon and nitrogen stable isotope analysis of 39 human bone and 8 animal samples from Middle Bronze Age (or Middle Helladic, MH, ca. 2100–1700 BC) Lerna, Greece. The isotopic data indicate that the humans had a C₃ terrestrial diet while certain individuals appear to have significant amounts of animal protein in their diet. With regard to weaning age, the isotopic values and the estimated age of early enamel disruptions suggest that solid foods were starting to be used as a substitute for breast milk at or before the ages of 2.5 and 3 years old.     

The European Merger Regime

Even though the European Union's merger regime has been warmly received, three distinct critiques of European policy in this field are identified. In acknowledging these critiques, this article reviews EU merger policy and suggests ways in which it might be reformed in the future. However, in order to understand the particular form that the policy now takes, emphasis must be placed on the context within which the European model of merger control has evolved. Three factors are considered: the rationale underpinning the policy; the origins of the policy as a hard-fought compromise among the (then) 12 members of the Union; and the role played by supranational actors in the implementation and enforcement of the policy. These three European characteristics of the policy help to explain the distinctiveness of the EU model.     

Introduction: Transnational retail and the global economy

The fastest retail-system transformation in history has beensweeping across the emerging markets of East Asia, Central andEastern Europe, and Latin America over the past decade withprofound impacts on economies and societies in those regions.Conceptualized by researchers in development studies and agriculturaleconomics as the ‘supermarket revolution in developingcountries’ (see, for example, Reardon et al., 2003; Reardon,2005; Reardon and Hopkins, 2006; Humphrey, 2007; Reardon etal., 2007), that transformation has been driven not only bydemand-side forces such as urbanization and income growth inthe emerging markets, but also by supply-side forces such