Towards the application of desorption electrospray ionisation mass spectrometry (DESI-MS) to the analysis of ancient proteins from artefacts

The detection and identification of protein residues from archaeological artefacts has been the subject of considerable controversy over the past two decades, this controversy spurring the application of a range of different analytical methods. Part of the debate surrounds the ability of the methods to uniquely identify original proteins. Another is the apparent contradiction between the ease of extraction in the laboratory of residues that have managed to survive in sediment over millennia; some studies use simple solvents including water, whilst others claim that efficient extraction requires the destruction of the artefact.Desorption electrospray ionisation mass spectrometry (DESI-MS) offers the potential to directly analyse proteins bound to the surface of artefacts. The method permits desorption of analytes under ambient conditions directly from a wide range of surfaces with little or no sample preparation. This has already led to its application in a range of areas, including forensic analysis of trace levels of narcotic drugs. It does not require destruction of the artefact (e.g. by drilling or dissolution) and can work in concert with other methods (for instance it could be used as a screening tool prior to lipid extraction).Here we report the construction of a DESI-MS interface and consider the potential of the method to contribute to the debate surrounding the preservation of proteins on artefacts. The DESI-MS interface was fitted to a conventional ion trap mass spectrometer and tested using peptides and proteins artificially applied to the surface of flint flakes and potsherd samples. A range of intact proteins was analysed; the DESI interface identified test proteins up to a maximum molecular weight of approximately 20 kDa. Peptides generated from these and larger proteins could be detected using DESI-MS following tryptic digestion in situ. The collected data allowed identification of the originating proteins. The potential applications to archaeological science of this new analytical tool are discussed.     

High modernism in the Arctic: planning Frobisher Bay and Inuvik

This paper considers Frobisher Bay and Inuvik, two Canadian Arctic towns, as examples of the high-modernist planning that swept the globe during the middle decades of the twentieth century, but also Cold War projects reflecting a sudden interest in the Arctic as a region of military significance. Building on the framework provided by James Scott in Seeing like a State (1998), the paper details the connections between modernization theory and Cold War militarism before turning to the strikingly parallel case studies. In each instance, federal officials proposed ambitious urban models designed to simultaneously overcome the hostility of a northern environment and catapult native northerners into conditions of modern living. While the limits and failings of such schemes varied by location, both sites were also laboratories for social scientists employed by the federal government to document and analyze the modernization of the Arctic. The work of these scholars represents a particularly rich and complex record of governmental interventions, tied variously to Cold War imperatives, in northern lives and landscapes at a time of great faith in the transformative power of modern engineering.     

Sourcing temper sands in ancient ceramics with U–Pb ages of detrital zircons: a southwest Pacific test case

Through use of methodology common in sedimentary geology, we apply U–Pb ages of detrital zircons to source nonlocal temper sand in an ancient ceramic assemblage recovered from Roviana Lagoon of the New Georgia Group in the Solomon Islands. Most potsherds from the Roviana Lagoon contain local volcanic sand as temper, but a small number of sherds contain anomalous granitic temper sand that does not appear to be local. To determine the origin of the anomalous temper, ages of zircons from the anomalous Roviana sherds are compared with ages of zircons in materials from Lizard Island off the Queensland coast and in sand from Muyuw Island in the Solomon Sea where generically similar granitic sands occur. U–Pb analyses of grains from the Roviana sherds yield Middle Miocene ages, while analyses of grains from Lizard Island granitic bedrock, sand, and local potsherds yield much older Permian-Triassic ages, disproving any possibility that the Roviana sherds were derived from Lizard Island, but suggesting local production of the Lizard Island sherds. Ages of grains in a sand sample from Muyuw Island are nearly identical to the ages of grains in the Roviana sherds. All grains in the Muyuw sand are Middle Miocene in age, overlapping closely with the Roviana age population. This strong similarity in detrital zircon signals indicates that the Roviana temper was likely derived from Muyuw Island sands. Our test case for the use of U–Pb ages of detrital zircons in sourcing temper sands is of only regional significance, and not of intrinsic global interest. The methodology, however, should find wide applicability for sourcing temper sands in many parts of the world, for it provides more specific data for the origins of tempers than either petrographic or chemical analysis.     

‘Rock-art’, ‘Animism’ and Two-way Thinking: Towards a Complementary Epistemology in the Understanding of Material Culture and ‘Rock-art’ of Hunting and Gathering People

In recent years, the concept of ‘animism’ has gained considerable popularity among archaeologists in exploring non-Western expressions of material culture. This development has also influenced recent academic approaches towards the study of ‘rock-art’ of people living as hunter and gatherers or in a hunting and gathering tradition. We argue here that attempts in this direction so far are generally compromised, because they fail to take Indigenous philosophies and intellectual contributions seriously. Any concern with Indigenous material expressions, including so-called rock-art, has to involve a critical re-assessment of academic discourse itself and a challenge to the primacy of Western scientific and literary, academic methodologies. With reference to the ‘rock-art’ and the world-view of the Ngarinyin (Kimberley, Northwest Australia), we present some preliminary thoughts for the development of an alternative interpretative framework, while offering a (much needed) legitimacy to another more balanced epistemology.     

Alexander Raven Thomson,Philosopher of the British Union of Fascists

This study surveys the career and political philosophy of Alexander Raven Thomson, one of Sir Oswald Mosley's lieutenants in the British Union of Fascists (BUF), the largest party on the extreme right in Britain in the interwar era. It explores key issues relating to the BUF, such as: What type of society did Thomson and the Blackshirts wish to establish in Britain? Who were some of the major domestic and international intellectual influences on him and the BUF? Was the BUF essentially a homegrown movement or was it, as critics argued, an imitation of Italian and German fascism? What does Thomson's career reveal about the inner politics of the movement? The aim of this article is to provide a better understanding of Thomson, one of the lesser-studied BUF officials, and of the movement to which he devoted his life.     

The Delicate Art of Aggression: Uzun Hasan's Fathnama to Qaytbay of 1469

Medieval Persian imperial correspondence (tarassul) remains relatively untapped as a source of historiographical information, due in no small part to its stylized and ornate character. This ornateness, far from being merely an obstructive husk to the useful pith of data within, constitutes a rich source of information in its own right; indeed, the formal aspects of a given letter may significantly alter the ostensible sense of the text. This study examines as a representative case a fathnama sent by Uzun Hasan to Qaytbay on the occasion of the former's victory over Sultan-Abu Sa‘id in 1469, here contextualized, translated and subjected to formal analysis with reference to contemporary insha’ manuals. For all its submissive rhetoric, the letter's aggressive intent is shown to be activated by its formal structure, which strategically deploys Uzun Hasanid messianic symbolism to challenge the ascendancy of the Mamluk state.