Mitochondrial DNA from 3000-year old chickens at the Teouma site,Vanuatu

Chickens were part of the Lapita cultural complex, transported into and through the Pacific by prehistoric colonists; as such they can be used as a proxy for tracking prehistoric migration and interaction. The Lapita site of Teouma in Vanuatu is well known for the recovery of complete dentate stamped pots and a cemetery containing the largest collection of Lapita period skeletons ever found. Chicken bones recovered from these excavations provide the first ancient DNA sequences from any commensal organism directly associated with a Lapita context. The ancient mtDNA sequences obtained from two Teouma chicken bones are compared with previously published archaeologically derived ancient DNA sequences to extend our understanding of the spread of chickens in Pacific prehistory. The results also show that the haplogroup E signature was present in very early populations of chickens transported into Remote Oceania. This study also adds to the suite of available data relating to isotopic signatures for commensal animals during the early settlement of Vanuatu and may reveal a different diet for Teouma chickens than those from other early prehistoric assemblages in the Pacific.     

Foreign bodies; or, how did Darwin invent the symptom?

Beginning with a discussion of the sources in Darwin's writing for Freud's theory of the hysterical symptom, this essay proceeds to a symptomatic reading of Darwin himself. With reference to "The Origin of Species," "The Descent of Man," and "The Expression of the Emotions," this essay shows that Darwin's theories of involuntary expressive behavior and of aesthetic preference in sexual selection are linked by their role in his understanding of racial difference and also by their reliance on the idea that learned habits can be inherited as instincts, a view often identified with Lamarck. They are thus at once theories of the foreign body and theories that appear as foreigners within the body of Darwin's work.     

Lapita subsistence strategies and food consumption patterns in the community of Teouma (Efate,Vanuatu)

The subsistence strategies of the Lapita populations (3100–2800 BP), the first colonisers of the pristine environments of the islands of Eastern Melanesia and Western Polynesia, have been a matter of ongoing debate for decades. Opinions have ranged between the two extremes of Lapita colonisers being either characterised as highly mobile foragers to fully horticultural communities. To further address the question, this paper presents stable carbon and nitrogen isotopic data obtained from analyses of human and animal collagen samples from the site of Teouma (Efate, Vanuatu) dated to between c. 3000–2500 BP. The isotopic signatures obtained from 28 samples (23 human and 5 animal), interpreted in combination with isotopic information from several coastal and insular environments, suggest a diet primarily made up of terrestrially derived animal protein with lesser contributions from vegetable produce and inshore marine species. Comparisons linking the isotopic data gleaned from the Teouma individuals and Lapita subsistence patterns reconstructed through archaeozoological and archaeobotanical remains support the hypothesis of a mixed economy, that included terrestrial foraging, inshore marine exploitation and a low level of food production for at least some of the earliest Lapita colonists in Vanuatu.     

Do Not Say That You Have Forgotten King and Father: Yunqi Zhuhong’s Chan Realism

This essay examines the late Ming-dynasty Chan master Yunqi Zhuhong’s commentary on the Brahma Net Sutra (Fanwangjing), which it takes up in order to explore his discourse concerning both Chan realism and his ensuing rejection of mainstream Chan gongan rhetoric. The Brahma Net Sutra contains a list of major and minor precepts governing proper morality for monastic and lay Buddhists. Zhuhong’s interpretation of the Twenty-First Minor Precept, which prohibits revenge, offers insight into his sense of political realism regarding the relationship between gradual teachings, provisional truths, and ultimate truth. His interpretation of the Tenth Minor Precept, which prohibits storing weapons, demonstrates his moral realism in contrast to Chan’s traditional use of pedagogical violence. Zhuhong’s realist discourse, influenced by the teachings of the Buddhist Vinaya as well as by engagement with Confucian ethics, presents an overlooked counter-narrative shift that contrasts with the emphasis on sudden enlightenment and antinomianism in Chan gongan discourse typical of the Tang and Song dynasties.     

The Use of Gasoline: Value, Oil, and the "American way of life"

Abstract:  While the critical literature has focused on the geography of oil production, the politics of "outrageous" gasoline prices in the United States provide a fertile path toward understanding the wider geography of petro-capitalism. Despite the deepening contradictions of US oil consumption, "pain at the pump" discourse projects a political sense of entitlement to low priced gasoline. I use a value-theoretical perspective to examine this politics as not only about the quantitative spectrum of price, but also the historical sedimentation of qualitative use-values inscribed in the commodity gasoline. Gasoline is analyzed both as a use-value among many within the postwar value of labor power and as a singular use-value fueling broader imaginaries of a national "American way of life." While use-value still represents an open site of cultural and political struggle infused within value itself, the case of gasoline illustrates how use-values are not automatically mobilized toward politically savory ends.     

Recovery of DNA from archaeological insect remains: first results,problems and potential

We report the recovery of short fragments of PCR amplifiable ancient DNA from exoskeletal fragments of the grain weevil Sitophilus granarius (L.) extracted from Roman and medieval deposits in Northern England. If DNA preservation in archaeological insect remains is widespread then many applications in the spheres of evolutionary studies and archaeology can be conceived, some of which are outlined.     

Towards the application of desorption electrospray ionisation mass spectrometry (DESI-MS) to the analysis of ancient proteins from artefacts

The detection and identification of protein residues from archaeological artefacts has been the subject of considerable controversy over the past two decades, this controversy spurring the application of a range of different analytical methods. Part of the debate surrounds the ability of the methods to uniquely identify original proteins. Another is the apparent contradiction between the ease of extraction in the laboratory of residues that have managed to survive in sediment over millennia; some studies use simple solvents including water, whilst others claim that efficient extraction requires the destruction of the artefact.Desorption electrospray ionisation mass spectrometry (DESI-MS) offers the potential to directly analyse proteins bound to the surface of artefacts. The method permits desorption of analytes under ambient conditions directly from a wide range of surfaces with little or no sample preparation. This has already led to its application in a range of areas, including forensic analysis of trace levels of narcotic drugs. It does not require destruction of the artefact (e.g. by drilling or dissolution) and can work in concert with other methods (for instance it could be used as a screening tool prior to lipid extraction).Here we report the construction of a DESI-MS interface and consider the potential of the method to contribute to the debate surrounding the preservation of proteins on artefacts. The DESI-MS interface was fitted to a conventional ion trap mass spectrometer and tested using peptides and proteins artificially applied to the surface of flint flakes and potsherd samples. A range of intact proteins was analysed; the DESI interface identified test proteins up to a maximum molecular weight of approximately 20 kDa. Peptides generated from these and larger proteins could be detected using DESI-MS following tryptic digestion in situ. The collected data allowed identification of the originating proteins. The potential applications to archaeological science of this new analytical tool are discussed.